Synthesis, characterization, and fluorescence and cytotoxicity studies of a tetrarhenium molecular rectangle

Inorganic Chemistry Communications

Volumn 10, Issue 7, 2007, Pages 821-824

  Orsa, Dejene K ^a   Haynes, Gregory K ^a   Pramanik, Saroj K ^a   Iwunze, Maurice O ^a   Greco, George E ^b   Krause, Jeanette A ^c   Ho, Douglas M ^d   Williams, Arthur L ^a   Hill, Dwayne A ^a   Mandal, Santosh K ^a  

^a MORGAN STATE UNIVERSITY   (United States)

^b GOUCHER COLLEGE   (United States)

^c UNIVERSITY OF CINCINNATI   (United States)

^d PRINCETON UNIVERSITY   (United States)

Author keywords

Cancer cell lines; Crystal structure; Fluorescence; Molecular rectangle; Rhenium

Indexed keywords

EID: 34249894172     PISSN: 13877003     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.inoche.2007.04.006     Document Type: Article

References (24)

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22. w = 0.0633, and goodness-of-fit = 1.154.
23. A steady-state fluorescence experiments were conducted on I using the Perkin-Elmer Luminescence Spectrophotometer, model LS 50B in a quartz cuvet of 1.0 cm in radiation pathlength. The excitation and emission slit widths were set at 6.0 nm, respectively. The solvents used in the experiments were dichloromethane, dioxane, dimethylformamide, dimethylsulfoxide, tetrahydrofuran, and acetonitrile.
24. 2 environment for various time periods. The dead cells were counted with either Trypan Blue Staining or WST-1/ECS solution. Trypan Blue Staining - Cell Viability: The medium was removed from each well and the cells were washed with PBS (phosphate buffer saline) twice. To each well 200 μL Trypan Blue Stain (0.4%) was added and incubated at room temperature for 5 min before recording the picture under microscope. The reactivity of Trypan Blue is based on negatively charged chromophore group, which reacts on damaged membrane i.e., the dead cells and viable cells will exclude the dye. WST-1/ECS solution: The trypsinized cells were counted on a haemocytometer. Approximately 50,000 cells were grown in 24-well plates and after confluent, the medium was replaced by serum starved medium (0.1% FBS) over night to bring the culture into a synchronized form. Next day, the medium was replaced with freshly prepared medium along with various doses of the molecular rectangle (I) which was previously dissolved in DMSO. After 72 h of exposure, the medium was aspirated and 100 μL/well WST-1/ECS solution was added to each well and incubated for 0.5-4 h in standard culture conditions. Before, checking the absorbancy, the plates were shaken for 1 min (detailed information is available from Roche Biochemicals, NJ).