Serum-free culturing of mammalian cells - Adaption to and cryopreservation in fully defined media

Altex

Volumn 24, Issue 2, 2007, Pages 110-116

  Hernández, Yanela González ^a   Fischer, René W ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

Animal component free; Chemically fully defined; Cryopreservation; Cryoprotectant; Serum and protein free

Indexed keywords

DIMETHYL SULFOXIDE; POLOXAMER;

ANIMAL CELL; ARTICLE; BIOREACTOR; CELL COUNT; CELL CULTURE; CELL GROWTH; CELL LINE; CRYOPRESERVATION; CRYOPROTECTION; CULTURE MEDIUM; CYTOLOGY; HUMAN; HUMAN CELL; MAMMAL CELL; NONHUMAN; SURVIVAL RATE;

ANIMALS; CELL CULTURE TECHNIQUES; CERCOPITHECUS AETHIOPS; COS CELLS; CRYOPRESERVATION; CULTURE MEDIA, SERUM-FREE; HAPLORHINI; INDICATORS AND REAGENTS; KIDNEY; MAMMALS; MICE; MICE, INBRED BALB C;

EID: 34249873005     PISSN: 09467785     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (9)

1. Han, B. and Bischof, J. C. (2004). Direct cell injury associated with eutectic crystallization during freezing. Cryobiology 48, 8-21
2. Handa-Corrigan, A., Emery, A. N. and Spear, R. E. (1989). Effect of gas-liquid interfaces on the growth of suspended animal cells: Mechanisms of cell damage by bubbles. Enzyme Microb. Technol. 11, 230-235.
3. Lakey, J. R., Rajotte, R. V., Fedorow, C. A. and Taylor, M. J. (2001). Islet cryopreservation using intracellular preservation solutions. Cell Transplantation 10, 583-589.
4. Mazur, P. (1970). Cryobiology: The freezing of biological systems. Science 168, 939-949.
5. Merten, O.-W., Petres, S. and Couvé, E. (1995). A simple serum-free freezing medium for serum-free cultured cells. Biologicals 23, 185-189.
6. Papoutsakis, E. T. (1991). Media additives for protecting freely suspended animal cells against agitation and aeration damage. Tibtech 9, 316-324.
7. Seeman, P. and Weinstein, J. (1966). I. Erythrocyte membrane stabilization by tranquilizers and antihistamines. Biochem. Pharmacol. 15, 1737-1752.
8. Van der Valk, J., Mellor D., Brands R. et al. (2004). The humane collection of fetal bovine serum and possibilities for serum-free cell and tissue culture. Toxicology in Vitro 18, 1-12.
9. Wu, J., Ruan, Q. and Lam H. Y. P. (1997). Effects of surface-active medium additives on insect cell surface hydrophobicity relating to cell protection against bubble damage. Enzyme Microb. Technol. 21, 341-348.