SEM and 3D synchrotron radiation micro-tomography in the study of bioceramic scaffolds for tissue-engineering applications

Biotechnology and Bioengineering

Volumn 97, Issue 3, 2007, Pages 638-648

  Peyrin, Françoise ^a,b,c   Mastrogiacomo, Maddalena ^d   Cancedda, Ranieri ^d   Martinetti, Roberta ^e  

^a UNIVERSITÉ DE LYON   (France)

^b CNRS   (France)

^c EUROPEAN SYNCHROTRON RADIATION FACILITY   (France)

^d UNIVERSITY OF GENOA   (Italy)

^e Fin Ceramica Faenza   (Italy)

Author keywords

Bioceramics; Hydroxyapatite; SEM; Synchrotron micro CT; Tissue engineering

Indexed keywords

BONE; CELL CULTURE; CHEMICAL ANALYSIS; HYDROXYAPATITE; SCANNING ELECTRON MICROSCOPY; SYNCHROTRON RADIATION; TISSUE ENGINEERING;

BIOLOGICAL MOLECULES; BONE DEPOSITION; SYNCHROTRON MICRO-CT;

BIOCERAMICS;

BIOCERAMICS; HYDROXYAPATITE;

ARTICLE; MICRO-COMPUTED TOMOGRAPHY; SCANNING ELECTRON MICROSCOPE; SYNCHROTRON RADIATION; THREE DIMENSIONAL IMAGING; TISSUE ENGINEERING;

BONE SUBSTITUTES; CERAMICS; DURAPATITE; IMAGING, THREE-DIMENSIONAL; MATERIALS TESTING; MICROSCOPY, ELECTRON, SCANNING; TISSUE ENGINEERING; TOMOGRAPHY, X-RAY COMPUTED;

EID: 34249814884     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21249     Document Type: Article

References (35)

1. Bernhardt R, Scharnweber D, Müller B, Thurner P, Schliephake H, Wyss P, Beckmann F, Goebbels J, Worch H. 2004. Comparison of microfocus-and synchrotron X-ray tomography for the analysis of osteointegration around Ti6Al4V implants. Eur Cell Mater 7:42-51.
2. Cartmell S, Huynh K, Lin A, Nagaraja S, Guldberg R. 2004. Quantitative microcomputed tomography analysis of mineralization within three-dimensional scaffolds in vitro. J Biomed Mater Res A 69A(1):97-104.
3. Chang BS, Lee CK, Hong KS, Youn HJ, Ryu HS, Chung SS, Park KW. 2000. Osteoconduction at porous hydroxyapatite with various pore configurations. Biomaterials 21(12):1291-1298.
4. Daculsi G, Laboux O, Malard O, Weiss P. 2003. Current state of the art of biphasic calcium phosphate bioceramics. J Mater Sci Mater Med 14(3): 195-200.
5. Friedman RJ, Bauer TW, Garg K, Jiang M, An YH, Draughn RA. 1995. Histological and mechanical comparison of hydroxyapatite-coated cobalt-chrome and titanium implants in the rabbit femur. J Appl Biomater 6(4):231-235.
6. Gronthos S. 2004. Reconstruction of human mandible by tissue engineering. Lancet 364(9436):735-736.
7. Heise U, Osborn JF, Duwe F. 1990. Hydroxyapatite ceramic as a bone substitute. Int Orthop 14(3):329-338.
8. Hildebrand T, Ruegsegger P. 1997. Quantification of bone microarchitecture with the structure model index. Comput Methods Biomech Biomed Engin 1(1):15-23.
9. Ho ST, Hutmacher DW. 2006. A comparison of micro CT with other techniques used in the characterization of scaffolds. Biomaterials 27(8): 1362-1376.
10. Hollister SJ. 2005. Porous scaffold design for tissue engineering. Nat Mater 4(7):518-524.
11. Jones AC, Milthorpe B, Averdunk H, Limaye A, Senden TJ, Sakellariou A, Sheppard AP, Sok RM, Knackstedt MA, Brandwood A. 2004. Analysis of 3D bone ingrowth into polymer scaffolds via micro-computed tomography imaging. Biomaterials 25(20):4947-4954.
12. Kon E, Muraglia A, Corsi A, Bianco P, Marcacci M, Martin I, Boyde A, Ruspantini I, Chistolini P, Rocca M, et al. 2000. Autologous bone marrow stromal cells loaded onto porous hydroxyapatite ceramic accelerate bone repair in critical-size defects of sheep long bones. J Biomed Mater Res 49(3):328-337.
13. Li S, De Wijn JR, Li J, Layrolle P, De Groot K. 2003. Macroporous biphasic calcium phosphate scaffold with high permeability/porosity ratio. Tissue Eng 9(3):535-548.
14. Lin ASP, Barrows TH, Cartmell SH, Guldberg RE. 2003. Microarchitectural and mechanical characterization of oriented porous polymer scaffolds. Biomaterials 24(3):481-489.
15. Martin I, Mastrogiacomo M, De Leo G, Muraglia A, Beltrame F, Cancedda R, Quarto R. 2002. Fluorescence microscopy imaging of bone for automated histomorphometry. Tissue Eng 8(5):847-852.
16. Martin-Badosa E, Elmoutaouakkil A, Nuzzo S, Amblard D, Vico L, Peyrin F. 2003. A method for the automatic characterization of bone architecture in 3D mice microtomographic images.
17. Martinetti R, Belpassi A, Nataloni A, Piconi C. 2001. Porous hydroxyapatite cell carrier for tissue engineering. Key Eng Mater 192-195:507-510.
18. Martinetti R, Belpassi A, Quarto R, Mastrogiacomo M, Cancedda R, Labanti M. 2004. Inspired porosity for cells and tissues. Key Eng Mater 254-256.
19. Martinetti R, Dolcini L, Mangano C. 2005. Physical and chemical aspects of a new porous hydroxyapatite. Anal Bioanal Chem 381(3):634-638.
20. Mastrogiacomo M, Komlev V, Hausard M, Peyrin F, Turquier F, Casari S, Cedola A, Rustichelli F, Cancedda R. 2004. Synchrotron radiation microtomography of bone engineered from bone marrow stromal cells. Tissue Eng 10(11-12):1767-1774.
21. Mastrogiacomo M, Muraglia A, Komlev V, Peyrin F, Rustichelli F, Crovace A, Cancedda R. 2005. Tissue engineering of bone: Search for a better scaffold. Orthod Craniofac Res 8(4):277-284.
22. Nuzzo S, Peyrin F, Cloetens P, Baruchel J, Boivin G. 2002. Quantification of the degree of mineralization of bone in three dimension using synchrotron radiation microtomography. Med Phys 19(11):2672-2681.
23. Odgaard A, Gundersen HJ. 1993. Quantification of connectivity in cancellous bone with special emphasis on 3-D reconstructions. Bone 14(2): 173-182.
24. Ozawa S, Kasugai S. 1996. Evaluation of implant materials (hydroxyapatite, glass-ceramics, titanium) in rat bone marrow stromal cell culture. Biomaterials 17(1):23-29.
25. Parfitt AM, Mathews CH, Villanueva AR, Kleerekoper M, Frame B, Rao DS. 1993. Relationships between surface, volume, and thickness of iliac trabecular bone in aging and in osteoporosis. Implications for the microanatomic and cellular mechanisms of bone loss. J Clin Invest 72(4):1396-1409.
26. Petite H, Viateau V, Bensaid W, Meunier A, de Pollak C, Bourguignon M, Oudina K, Sedel L, Guillemín G. 2000. Tissue-engineered bone regeneration. Nat Biotechnol 18(9):929-930.
27. Quarto R, Mastrogiacomo M, Cancedda R, Kutepov SM, Mukhachev V, Lavroukov A, Kon E, Marcacci M. 2001. Repair of large bone defects with the use of autologous bone marrow stromal cells. N Engl J Med 344(5):385-386.
28. Ruegsegger P, Koller B, Muller R. 1996. A microtomographic system for the nondestructive evaluation of bone architecture. Calcif Tissue Int 58(1):24-29.
29. Salome M, Peyrin F, Cloetens P, Odet C, Laval-Jeantet AM, Baruchel J, Spanne P. 1999. A synchrotron radiation microtomography system for the analysis of trabecular bone samples. Med Phys 26(10):2194-2204.
30. Serra J. 1982. Image analysis and mathematical morphology. London: Academic Press. 600p
31. Simmons CA, Hipp JA. 1997. Method-based differences in the automated analysis of the three-dimensional morphology of trabecular bone. J Bone Miner Res 12(6):942-947.
32. Tampieri A, Celotti G, Sprio S, Delcogliano A, Franzese S. 2001. Porosity-graded hydroxyapatite ceramics to replace natural bone. Biomaterials 22(11):1365-1370.
33. Thurner P, Müller R, Raeber G, Sennhauser U, Hubbell JA. 2005. 3D morphology of cell cultures: A quantitative approach using micrometer synchrotron light tomography. Microsc Res Tech 66(6):289-298.
34. 2O-CaO-MgO glass-ceramic bioactive scaffolds for bone substitutes. J Mater Sci Mater Med 16(9):909-917.
35. Weiss P, Obadia L, Magne D, Bourges X, Rau C, Weitkamp T, Khairoun I, Bouler JM, Chappard D, Gauthier O, Dalcusi G. 2003. Synchrotron X-ray microtomography (on a micron scale) provides three-dimensional imaging representation of bone ingrowth in calcium phosphate biomaterials. Biomaterials 24(25):4591-4601.