Expression and purification of F-plasmid RepE and preliminary X-ray crystallographic study of its complex with operator DNA

Acta Crystallographica Section F: Structural Biology and Crystallization Communications

Volumn 63, Issue 4, 2007, Pages 346-349

  Nakamura, Akira ^a   Wada, Chieko ^a   Miki, Kunio ^a,b  

^a KYOTO UNIVERSITY   (Japan)

^b Harima Institute ^*  (Japan)

Author keywords

High salt purification; RepE dimer; RepE operator complex; Transcriptional repressor

Indexed keywords

ESCHERICHIA COLI;

BACTERIAL DNA; DNA BINDING PROTEIN; ESCHERICHIA COLI PROTEIN; REPE PROTEIN, E COLI; REPRESSOR PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CHEMISTRY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CONFORMATION; X RAY CRYSTALLOGRAPHY;

BASE SEQUENCE; CRYSTALLOGRAPHY, X-RAY; DNA, BACTERIAL; DNA-BINDING PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; PLASMIDS; PROTEIN CONFORMATION; REPRESSOR PROTEINS;

EID: 34249734768     PISSN: 17443091     EISSN: 17443091     Source Type: Journal    
DOI: 10.1107/S1744309107012894     Document Type: Article

References (22)

1. Collaborative Computational Project, Number 4 (1994). Acta Cryst. D50, 760-763.
2. Harp, J. M., Timm, D. E. & Bunick, G. J. (1998). Acta Cryst. D54, 622-628.
3. Heras, B. & Martin, J. L. (2005). Acta Cryst. D61, 1173-1180.
4. Ishiai, M., Wada, C., Kawasaki, Y. & Yura, T. (1994). Proc. Natl Acad. Sci. USA, 91, 3839-3843.
5. Joachimiak, A. & Sigler, P. B. (1991). Methods Enzymol. 208, 82-99.
6. Kawasaki, Y., Matsunaga, F., Kano, Y., Yura, T. & Wada, C. (1996). Mol. Gen. Genet. 253, 42-49.
7. Kawasaki, Y., Wada, C. & Yura, T. (1990). Mol. Gen. Genet. 220, 277-282.
8. Kawasaki, Y., Wada, C. & Yura, T. (1991). J. Bacteriol. 173, 1064-1072.
9. Kawasaki, Y., Wada, C. & Yura, T. (1992). J. Biol. Chem. 267, 11520-11524.
10. Kline, B. C. (1985). Plasmid, 14, 1-16.
11. Kline, B. C., Sandhu, G. S., Eckloff, B. W. & Aleff, R. A. (1992). J. Bacteriol. 174, 3004-3010.
12. Komori, H., Matsunaga, F., Higuchi, Y., Ishiai, M., Wada, C. & Miki, K. (1999). EMBO J. 18, 4597-4607.
13. Komori, H., Sasai, N., Matsunaga, F., Wada, C. & Miki, K. (1999). J. Biochem. (Tokyo), 125, 24-26.
14. Masson, L. & Ray, D. S. (1986). Nucleic Acids Res. 14, 5693-5711.
15. Masson, L. & Ray, D. S. (1988). Nucleic Acids Res. 16, 413-424.
16. Matsunaga, F., Ishiai, M., Kobayashi, G., Uga, H., Yura, T. & Wada, C. (1997). J. Mol. Biol. 274, 27-38.
17. Matsunaga, F., Kawasaki, Y., Ishiai, M., Nishikawa, K., Yura, T. & Wada, C. (1995). J. Bacteriol. 177, 1994-2001.
18. Matthews, B. W. (1968). J. Mol. Biol. 33, 491-497.
19. Otwinowski, Z. & Minor, W. (1997). Methods Enzymol. 276, 307-326.
20. Rokeach, L. A., Sogaard-Andersen, L. & Molin, S. (1985). J. Bacteriol. 164, 1262-1270.
21. Schick, B. & Jurnak, F. (1994). Acta Cryst. D50, 563-568.
22. Tokino, T., Murotsu, T. & Matsubara, K. (1986). Proc. Natl Acad. Sci. USA, 83, 4109-4113.