Differentiation of Bacillus anthracis, B. cereus, and B. thuringiensis by using pulsed-field gel electrophoresis

Applied and Environmental Microbiology

Volumn 73, Issue 10, 2007, Pages 3446-3449

  Zhong, Wenwan ^a,b   Shou, Yulin ^a   Yoshida, Thomas M ^a   Marrone, Babetta L ^a  

^a LOS ALAMOS NATIONAL LABORATORY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CLUSTER ANALYSIS; DIGESTIVE SYSTEM; ELECTROPHORESIS; ENZYMES; GENES;

BACILLUS ANTHRACIS; ENZYME DIGESTION PATTERNS; HOMOLOGY; PULSED FIELD GEL ELECTROPHORESIS (PFGE);

BACTERIA;

BACTERIUM; CLUSTER ANALYSIS; DIFFERENTIATION; ELECTROKINESIS; GENOME; HOMOLOGY;

ARTICLE; BACILLUS ANTHRACIS; BACILLUS ATROPHAEUS; BACILLUS CEREUS; BACILLUS THURINGIENSIS; BACTERIAL GENOME; BACTERIAL STRAIN; BACTERIUM ISOLATE; GENOME ANALYSIS; NONHUMAN; PULSED FIELD GEL ELECTROPHORESIS; SPECIES DIFFERENTIATION;

BACILLUS ANTHRACIS; BACILLUS CEREUS; BACILLUS THURINGIENSIS; BACTERIAL TYPING TECHNIQUES; CLUSTER ANALYSIS; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA, BACTERIAL; ELECTROPHORESIS, GEL, PULSED-FIELD; GENOTYPE; PHYLOGENY;

BACILLUS ANTHRACIS; BACILLUS CEREUS; BACILLUS THURINGIENSIS;

EID: 34249691947     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02478-06     Document Type: Article

References (16)

1. Carlson, C. R., D. A. Caugant, and A.-B. Kolstø. 1994. Genotypic diversity among Bacillus cereus and Bacillus thuringiensis strains. Appl. Environ. Microbiol. 60:1719-1725.
2. Daffonchio, D., A. Cherif, and S. Borin. 2000. Homoduplex and heteroduplex polymorphisms of the amplified ribosomal 16S-23S internal transcribed spacers describe genetic relationships in the "Bacillus cereus group." Appl. Environ. Microbiol. 66:5460-5468.
3. Ferris, M. M., X. Yan, R. C. Habbersett, Y. Shou, C. L. Lemanski, J. H. Jett, T. M. Yoshida, and B. L. Marrone. 2004. Performance assessment of DNA fragment sizing by high-sensitivity flow cytometry and pulsed-field gel electrophoresis. J. Clin. Microbiol. 42:1965-1976.
4. Han, C. S., G. Xie, J. F. Challacombe, M. R. Altherr, S. S. Bhotika, D. Bruce, C. S. Campbell, M. L. Campbell, J. Chen, O. Chertkov, C. Cleland, M. Dimitrijevic, N. A. Doggett, J. J. Fawcett, T. Glavina, L. A. Goodwin, K. K. Hill, P. Hitchcock, P. J. Jackson, P. Keim, A. R. Kewalramani, J. Longmire, S. Lucas, S. Malfatti, K. McMurry, L. J. Meincke, M. Misra, B. L. Moseman, M. Mundt, A. C. Muck, R. T. Okinaka, B. Parson-Quintana, L. P. Reilly, P. Richardson, D. L. Robinson, E. Rubin, E. Saunders, R. Tapia, J. G. Tesmer, N. Thayer, L. S. Thompson, H. Tice, L. O. Ticknor, P. L. Wills, T. S. Brettin, and P. Gilna. 2006. Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis. J. Bacteriol. 188:3382-3390.
5. Helgason, E., O. A. Okstad, D. A. Caugant, H. A. Johansen, A. Fouet, M. Mock, I. Hegna, and A.-B. Kolstø. 2000. Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis - one species on the basis of genetic evidence. Appl. Environ. Microbiol. 66:2627-2630.
6. Hill, K. K., L. O. Ticknor, R. T. Okinaka, M. Asay, H. Blair, K. A. Bliss, M. Laker, P. E. Pardington, A. P. Richardson, M. Tonks, D. J. Beecher, J. D. Kemp, A. B. Kolstø, A. C. L. Wong, P. Keim, and P. J. Jackson. 2004. Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Appl. Environ. Microbiol. 70:1068-1080.
7. Hoffmaster, A. R., J. Ravel, D. A. Rasko, G. D. Chapman, M. D. Chute, C. K. Marston, B. K. De, C. T. Sacchi, C. Fitzgerald, L. W. Mayer, M. C. J. Maiden, F. G. Priest, M. Barker, L. Jiang, R. Z. Cer, J. Rilsone, S. N. Peterson, R. S. Weyant, D. R. Galloway, T. D. Read, T. Popovic, and C. M. Fraser. 2004. Identification of anthrax toxin genes in a Bacillus cereus associated with an illness resembling inhalation anthrax. Proc. Natl. Acad. Sci. USA 101:8449-8454.
8. Jernigan, J. A., D. S. Stephens, D. A. Ashford, C. Omenaca, M. S. Topiel, M. Galbraith, M. Tapper, T. L. Fisk, S. Zaki, T. Popovic, R. F. Meyer, C. P. Quinn, S. A. Harper, S. K. Fridkin, J. J. Sejvar, C. W. Shepard, M. McConnell, J. Guarner, W. J. Shieh, J. M. Malecki, J. L. Gerberding, J. M. Hughes, and B. A. Perkins. 2001. Bioterrorism-related inhalational anthrax: the first 10 cases reported in the United States. Emerg. Infect. Dis. 7:933-944.
9. Keim, P., A. Kalif, J. M. Schupp, K. K. Hill, S. E. Travis, K. Richmond, D. M. Adair, M. E. Hugh-Jones, C. R. Kuske, and P. Jackson. 1997. Molecular evolution and diversity in Bacillus anthracis as detected by amplified fragment length polymorphism markers. J. Bacteriol. 179:818-824.
10. Keim, P., L. B. Price, A. M. Klevytska, K. L. Smith, J. M. Schupp, R. Okinaka, P. J. Jackson, and M. E. Hugh-Jones. 2000. Multiple-locus variable-number tandem repeat analysis reveals genetic relationships within Bacillus anthracis. J. Bacteriol. 182:2928-2936.
11. Klaassen, C. H. W., H. A. van Hanneke, and A. M. Horrevorts. 2002. Molecular fingerprinting of Clostridium difficile isolates: pulsed-field gel electrophoresis versus amplified fragment length polymorphism. J. Clin. Microbiol. 40:101-104.
12. Radnedge, L., P. G. Agron, K. K. Hill, P. J. Jackson, L. O. Ticknor, P. Keim, and G. L. Andersen. 2003. Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Appl. Environ. Microbiol. 69:2755-2764.
13. Rasko, D. A., M. R. Altherr, C. S. Han, and J. Ravel. 2005. Genomics of the Bacillus cereus group of organisms. FEMS Microbiol. Rev. 29:303-329.
14. Read, T. D., S. L. Salzberg, M. Pop, M. Shumway, L. Umayam, L. Jiang, E. Holtzapple, J. D. Busch, K. L. Smith, J. M. Schupp, D. Solomon, P. Keim, and C. M. Fraser. 2002. Comparative genome sequencing for discovery of novel polymorphisms in Bacillus anthracis. Science 296:2028-2032.
15. Swaminathan, B., T. J. Barrett, S. B. Hunter, and R. V. Tauxe. 2001. PulseNet: the molecular subtyping network for foodborne bacterial disease surveillance, United States. Emerg. Infect. Dis. 7:382-389.
16. Vilas-Boas, G., V. Sanchis, D. Lereclus, and M. V. Lemos. 2002. Genetic differentiation between sympatric populations of Bacillus cereus and Bacillus thuringiensis. Appl. Environ. Microbiol. 68:1414-1424.