Expression of furin-linked Fab fragments against anthrax toxin in a single mammalian expression vector

Protein Expression and Purification

Volumn 54, Issue 2, 2007, Pages 261-266

  Reed, Christopher D ^a   Rast, Heidi ^a   Hu, Wei gang ^b   Mah, David ^c   Nagata, Les ^b   Masri, Saad A ^a  

^a Canadian Food Inspection Agency   (Canada)

^b DEFENCE RESEARCH AND DEVELOPMENT CANADA   (Canada)

^c Canada West Biosciences Inc   (Canada)

Author keywords

Antibodies; Antibody expression Fab; Bacillus anthracis; Furin; Protective antigen

Indexed keywords

ANTHRAX TOXIN; BACTERIAL ANTIGEN; BACTERIAL TOXIN; FURIN; IMMUNOGLOBULIN F(AB) FRAGMENT;

ANIMAL; ARTICLE; BIOSYNTHESIS; CHO CELL; CRICETULUS; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; HAMSTER; HUMAN; IMMUNOLOGY; WESTERN BLOTTING;

ANIMALS; ANTIGENS, BACTERIAL; BACTERIAL TOXINS; BLOTTING, WESTERN; CHO CELLS; CRICETINAE; CRICETULUS; FURIN; GENETIC VECTORS; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; TRANSFECTION;

BACILLUS ANTHRACIS; MAMMALIA;

EID: 34249314784     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.03.007     Document Type: Article

References (24)

1. Christopher G.W., Cieslak T.J., Pavlin J.A., and Eitzen E.M. Biological warfare: a historical perspective. JAMA 278 (1997) 412-417
2. Mourez M. Anthrax toxins. Rev. Physiol. Biochem. Pharmacol. 152 (2004) 135-164
3. Gilligan P.H. Therapeutic challenges posed by biological bioterrorism threats. Curr. Opin. Microbiol. 5 (2002) 489-495
4. Albrink W.S. Pathogenesis of inhalation anthrax. Micro. Miol. Bio. Rev. 25 (1961) 262-267
5. Kimpel K.R., Molloy S.S., Thomas G., and Leppla S.H. Anthrax toxin protective antigen is activated by cell surface protease with the sequence specificity and catalytic properties of furin. Proc. Natl. Acad. Sci. USA 89 (1992) 10277-10281
6. Scobie H.M., and Young J.T. Interactions between anthrax toxin receptors and protective antigen. Curr. Opin. Mirco. 8 (2005) 106-112
7. Rainey G., and Young J. Antitoxins: Novel strategies to target agents of bioterrorism. Nature Rev. Mircobiol. 2 (2004) 721-726
8. Giannasca P.J., and Warny M. Active and passive immunization against Clostridium difficile diarrhea and colitis. Vaccine 22 (2004) 848-856
9. Subbarao K., McAuliffe J., Vogel L., Fahle G., Fischer S., Tatti K., Packard M., Shieh W.J., Zaki S., and Murphy B. Prior infection and passive transfer of neutralizing antibody prevent replication of severe acute respiratory syndrome coronavirus in the respiratory tract of mice. J. Virology 78 (2004) 3572-3577
10. Vollmers H.P., and Brandlein S. Nature's best weapons to fight cancer: revival of human monoclonal IgM antibodies. Hum. Antibodies 11 (2002) 131-142
11. Wild M.A., Xin H., Maruyama T., Nolan M.J., Calveley P.M., Malone J.D., Wallace M.R., and Bowdish K.S. Human antibodies from immunized donors are protective against anthrax toxin in vivo. Nat. Biotech. 21 (2003) 1305-1306
12. Jakobovits A. Production of fully human antibodies by transgenic mice. Curr. Opin. Biotech. 6 (1995) 561-566
13. Longberg N., and Huszar D. Human antibodies from trans-genic mice. Int. Rev. Immunol. 13 (1995) 65-93
14. Masri S.A., Rast H., Wei-gang H., Mah D., and Nagata L. Cloning and expression in E. coli. of a functional Fab fragment obtained from single human lymphocyte against Bacillus anthracis. Mol. Immuno. 44 (2007) 2111-2116
15. Mallender W.D., and Voss Jr. E.W. Construction, expression, and activity of a bivalent bispecific single-chain antibody. J. Biol. Chem. 269 (1994) 199-206
16. Willems A., Leoen J., Schoonooghe S., Grooten J., and Mertens N. Optimizing expression and purification from cell culture medium of trispecific recombinant antibody derivatives. J. Chromatogr B. 786 (2003) 161-176
17. Simmons L.C., Reilly D., Klimowski L., Raju T.S., Meng G., Sims P., Hong K., Shields R.L., Damico L.A., Rancatore P., and Yansura D.G. Expression of full-length immunoglobulins in Escherichia coli: rapid and efficient production of aglycosylated antibodies. J. Immunol. Methods 263 (2002) 133-147
18. Laird M.W., Cope K., Atkinson R., Donahoe M., Johnson K., and Melick M. Keratinocyte growth factor-2 Production in an ompT-deficient Escherichia coli K-12 mutant. Biotechnol. Prog. 20 (2004) 44-50
19. Thomas G. Furin at the cuuting edge: from protein traffic to embryogenesis and disease. Nat. Rev. Mol. Cell Biol. 3 (2002) 753-766
20. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
21. Rotz L.D., Khan A.S., Lillibridge S.R., Ostroff S.M., and Hughes J.M. Public health assessment of potential biological terrorism agents. Emerg. Infect. Dis. 8 (2002) 225-230
22. Holliger P., and Hudson P.J. Engineered antibody fragments and the rise of the single domains. Nat. Biotech. 23 (2005) 1126-1136
23. Wu A.M., Chen W., Raubitschek A., Williams L.E., Neumaier M., Fischer R., Hu S., Odom-Maryon T., Wong J.Y.C., and Shively J.E. Tumor localization of anti-CEA single chain FVs: improved targeting by non covalent dimmers. Immunotech. 2 (1996) 21-36
24. Lagerkvist A.C., Furebring C., and Borrebaeck C.A.K. Single antigen-specific B cells used to generate Fab fragments using CD-40-mediated amplification or direct PCR cloning. Bio Technique 18 (1995) 862-866