The epidermis of scales in gecko lizards contains multiple forms of β-keratins including basic glycine-proline-serine-rich proteins

Journal of Proteome Research

Volumn 6, Issue 5, 2007, Pages 1792-1805

  Toni, M ^a   Dalla Valle, L ^b   Alibardi, L ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b UNIVERSITY OF PADOVA   (Italy)

Author keywords

keratin sequences; Cornification; Epidermis; Gecko lizards; Glycine proline rich proteins; Setae

Indexed keywords

GLYCINE; KERATIN; PROLINE; SERINE;

AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BIRD; ELECTROPHORESIS; EPIDERMIS; IMMUNOBLOTTING; LIZARD; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN ISOLATION; PROTEIN STRUCTURE; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANIMALS; BETA-KERATINS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; EPIDERMIS; FOOT; GLYCINE; LIZARDS; MOLECULAR SEQUENCE DATA; PROLINE; PROTEIN CONFORMATION; PROTEIN ISOFORMS; SERINE;

AVES; GEKKONIDAE; REPTILIA; SQUAMATA;

EID: 34249295523     PISSN: 15353893     EISSN: None     Source Type: Journal    
DOI: 10.1021/pr060626+     Document Type: Article

References (42)

1. Maderson, P. F.; Rabinowitz, T.; Tandler, B.; Alibardi, L. Ultrastructural contributions to an understanding of the cellular mechanisms involved in lizard skin shedding with comments on the function and evolution of a unique lepidosaurian phenomenon. J. Morphol. 1998, 236, 1-24.
2. Maderson, P. F. Lizard glands and lizard hands: models for evolutionary study. Form. Function 1970, 3, 179-204.
3. Ernst, V.; Ruibal, R. The structure and development of the digital lamellae of lizards. J. Morphol. 1966, 120, 233-65.
4. Hiller, U. Licht-und elektronenmikroskopische untersuchungen zur haftborstenentwickllung bei Tarentola mauritanica (Reptilia, Gekkonidae). Z. Morphol. Tiere 1972, 73, 263-78.
5. Alibardi, L. Morphogenesis of the digital pad lamellae in the embryo of the lizard Anolis lineatopus. J. Zool. (London) 1998, 243, 47-55.
6. Alibardi, L. Ultrastructural and autoradiographic analysis of setae development in the embryonic pad lamellae of the lizard Anolis lineatopus. Ann. Sci. Nat. (Zool.) 1997, 18, 51-61.
7. Autumn, K.; Sitti, M.; Liang, Y. A.; Peattie, A. M.; Hansen, W. R.; Sponberg, S.; Kenny, T. W.; Fearing, R.; Israelachvili, J. N.; Full, R. J. Evidence for van der Waals adhesion in gecko setae. Proc. Natl. Acad. Sci. U.S.A. 2002, 99, 12252-6.
8. Autumn, K.; Liang, Y. A.; Hsieh, S. T.; Zesch, W.; Chan, W. P.; Kenny, T. W.; Fearing, R.; Full, R. J. Adhesive force of a single gecko foot-hair. Nature 2000, 405, 681-5.
9. Huber, G.; Mantz, H.; Spolenak, R.; Mecke, K.; Jacobs, K.; Gorb, S. N.; Arzt, E. Evidence for capillarity contributions to gecko adhesion from single spatula nanomechanical measurements. Proc. Natl. Acad. Sci. U.S.A. 2005, 102, 16293-6.
10. Huber, G.; Gorb S. N.; Spolenak, R.; Arzt, E. Resolving the nanoscale adhesion of individual gecko spatulae by atomic force microscopy. Biol. Lett. 2005, 1, 2-4.
11. Bonser, R. H.; Farrent, J. W. Influence of hydration on the mechanical performance of duck down feathers. Br. Poult. Sci. 2001, 42, 271-3.
12. Taylor, A.; Bonser, R. H.; Farrent, J. W. The influence of hydration on the tensile and compressive properties of avian keratinous tissues. J. Mater. Sci. 2004, 39, 939-42.
13. Alibardi, L. Ultrastructural autoradiographic and immunocytochemical analysis of setae formation and keratinization in the digital pads of the gecko Hemidactylus turcicus (Gekkonidae, Reptilia). Tissue Cell 2003, 35, 288-96.
14. Thorpe, R. S.; Giddings, M. R. A novel biochemical systematic technique for herpetology based on epidermal keratins. Experientia 1981, 37, 700-2.
15. Alibardi, L.; Toni, M. Distribution and characterization of proteins associated with cornification in the epidermis of gecko lizard. Tissue Cell 2005, 37, 423-33.
16. Rizzo, N. W.; Gardner, K. H.; Walls, D. J.; Keiper-Hrynko, N. M.; Ganzke, T. S.; Hallahan, D. L. Characterization of the structure and composition of gecko adhesive setae. J. R. Soc., Interface 2006, 3, 441-51.
17. Wyld, J. A.; Brush, A. H. The molecular heterogeneity and diversity of reptilian keratins. J. Mol. Evol. 1979, 12, 331-47.
18. Wyld, J. A.; Brush, A. H. Keratin diversity in the reptilian epidermis. J. Exp. Zool. 1983, 225, 387-96.
19. Gregg, K.; Rogers, G. Feather keratins: composition, structure and biogenesis. In Biology of the Integument, Vertebrates; Bereither-Hahn, J.. Matoltsy, G.. Sylvia-Richards, K.. Eds.; Springer-Verlag: Berlin-Heidelberg-New York, 1986; pp 666-94.
20. Fraser, R. D.; Parry, D. A. The molecular structure of reptilian keratin. Int. J. Biol. Macromol. 1996, 19, 207-11.
21. Sawyer, R. H.; Glenn, T. C.; French, J. O.; Mays, B.; Shames, R. B.; Barnes, G. L.; Rhodes, W.; Ishikawa, Y. The expression of beta (b) keratins in the epidermal appendages of reptiles and birds. Amer. Zool. 2000, 40, 530-39.
22. Dalla Valle, L.; Toffolo, V.; Belvedere, P.; Alibardi, L. Isolation of a mRNA encoding a glycine-proline-rich beta-keratin expressed in the regenerating epidermis of lizard. Dev. Dyn. 2005, 234, 934-47.
23. Alibardi, L.; Dalla Valle, L.; Toffolo, V.; Toni, M. Scale keratin in lizard epidermis reveals amino acid regions homologous with avian and mammalian epidermal proteins. Anat. Rec., Part A 2006, 288, 734-52.
24. Dalla Valle, L.; Nardi, A.; Toffolo, V.; Niero, C.; Toni, M.; Alibardi, L. Cloning and characterization of scale β-keratins in the differentiating epidermis of geckos show they are glycine-proline-serine-rich proteins with a central motif homologous to avian beta-keratins. Dev. Dyn. 2007, 236, 374-88.
25. Scala, C.; Cenacchi, G.; Ferrari, C.; Pasquinelli, G.; Preda, P.; Manara, G. C. A new acrylic resin formulation: a useful tool for histological, ultrastructural, and immunocytochemical investigations. J. Histochem. Cytochem. 1992, 40, 1799-804.
26. Alibardi, L.; Toni, M. Immunological characterization and fine localization of a lizard beta-keratin. J. Exp. Zool., Part B 2006, 306, 528-38.
27. Sawyer, R. H.; Glenn, T. C.; French, J. O.; Knapp, L. W. Developing antibodies to synthetic peptides based on comparative DNA sequencing of multigene families. Methods Enzymol. 2005, 395, 636-52.
28. Sybert, V. P.; Dale, B. A.; Holbrook, K. A. Ichthyosis vulgaris: identification of a defect in synthesis of filaggrin correlated with an absence of keratohyaline granules. J. Invest. Dermatol. 1985, 84, 191-4.
29. Laemmli, U. K. Cleavage of structural proteins during the assemblage of the head of the bacteriophage T4. Nature 1970, 227, 680-85.
30. O'Guin, W. M.; Sawyer, R. H. Avian scale development. VII. Relationships between morphogenetic and biosynthetic differentiation. Dev. Biol. 1982, 89, 485-92.
31. McGuffin, L. The PSIPRED protein structure prediction server. Bioinformatics 2000, 16, 404-5.
32. Alibardi, L.; Toni, M. Cytochemical, biochemical and molecular aspects of the process of keratinization in the epidermis of reptilian scales. Prog. Histochem. Cytochem. 2006, 40, 73-134.
33. Brush, A. H. The origin of feather: a novel approach. In Avian Biology; Farner, D., Kling, J., Parker, K., Eds.; Academic Press: New York, 1993; pp 121-62.
34. Knapp, L. W.; Shames, R. B.; Barnes, G. L.; Sawyer, R. H. Region-specific patterns of beta keratin expression during avian skin development. Dev. Dyn. 1993, 196, 283-90.
35. Inglis, A.; Gillespie, J. M.; Roxburgh, C.; Whittaker, L.; Casagranda, F. Sequence of a glycine-rich protein from lizard claw: unusual dilute acid and heptafluorobutyric acid cleavage. In Protein, Structure and Function; L'Italien, Ed.; Plenum Press: New York-London, 1987; pp 757-64.
36. Baden, H. P.; Maderson, P. F. Morphological and biophysical identification of fibrous proteins in the amniote epidermis. J. Exp. Zool. 1970, 174, 225-32.
37. Baden, H.; Sviokla, S.; Roth, I. The structural protein of reptilian scales. J. Exp. Zool. 1974, 187, 287-94.
38. Gillespie, J. M. The structural proteins of hair: isolation, characterization and regulation of biosynthesis. In Physiology, Biochemistry and Molecular Biology of the Skin; Goldsmith, L., Ed.; Oxford University Press: Oxford, U.K., 1991; pp 625-59.
39. Powell, B.; Rogers, G. Differentiation in hard keratin tissues: hair and related structure. In The Keratinocyte Handbook; Leigh, I., Lane, B., Watt, F., Eds.; Cambridge University Press, Cambridge, U.K., 1994; pp 401-36.
40. Blom, N.; Gammeltoft, S.; Brunak, S. Sequence and structure-based prediction of eukaryotic protein phosphorylation sites. J. Mol. Biol. 1999, 294, 1351-62.
41. Frenkel, M. J.; Gillespie, J. M. The proteins of the keratin component of bird's beaks. Aust. J. Biol. Sci. 1976, 29, 467-79.
42. Gregg, K.; Wilton, S.; Parry, D. A.; Rogers, G. A comparison of genomic coding sequences for feather and scale keratins: structural and evolutionary implications. EMBO J. 1984, 3, 175-78.