The effect of neem (Azadirachta indica) leaves extract in alpha-fetoprotein serum concentration, glutathione S-transferase and glutathione peroxidase activity in hepatocarcinogenesis induced rats

International Journal of Cancer Research

Volumn 3, Issue 2, 2007, Pages 111-118

  Manal, Mohamed Elhassan Taha ^a   Hanachi, P ^b   Patimah, I ^a   Siddig, I A ^a   Fauziah, O ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

^b Department of Biomedical Sciences   (Iran)

Author keywords

Azadarichta indica; GPx and AFP; GST; Liver cancer

Indexed keywords

ALPHA FETOPROTEIN; ANTINEOPLASTIC AGENT; AZADIRACHTA INDICA EXTRACT; CORN OIL; DIETHYLNITROSAMINE; GLUTATHIONE PEROXIDASE; GLUTATHIONE TRANSFERASE; N (2 FLUORENYL)ACETAMIDE; PLANT EXTRACT; TUMOR MARKER;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTINEOPLASTIC ACTIVITY; ARTICLE; CONTROLLED STUDY; DRUG EFFICACY; ENZYME ACTIVITY; LIVER CANCER; LIVER CARCINOGENESIS; LIVER CYTOSOL; MALE; NONHUMAN; PLANT LEAF; PROTEIN BLOOD LEVEL; RAT;

EID: 34249292992     PISSN: 18119727     EISSN: 18119735     Source Type: Journal    
DOI: 10.3923/ijcr.2007.111.118     Document Type: Article

References (33)

1. Ahluwalia, M. and E. Farber, 1984. Alteration in glutathione status in early hyperplastic nodules. Proc. Am. Assoc. Cancer Res., 25: 15-17.
2. Balasenthil, S., S. Arivazhagan, C.R. Ramachandran, V. Ramachadran and S. Nagini, 1999. Chemopreventive potential of NEEM (Azadirachta indica) on 7,12-dimethylbenz(a) anthrance (DMBA) induced hamster buccal pouch carcinogenesis. J. Ethnopharmacol., 67: 189-195.
3. Bhanwara, S., J. Singh and P. Khosla, 2000. Effect of Azadirachta indica (Neem) leaf aqueous extract on paracetamol induced liver damage in rats. Indian J. Physiol. Pharmacol., 44: 64-68.
4. Bradford, M.M., 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem., 50: 249-253.
5. Brahmachari, G., 2004. A Neem - An omnipotent plant: A retrospection Chembiochem., 2: 408-421.
6. Chasseaud, L. F., 1979. The role of glutathione and glutathione S-transferases in the metabolism of chemical carcinogens and other electrophilic agents. Adv. Cancer Res., 29: 175-274.
7. Conney, A.H., Z.Y. Wang, M.T. Huang, C.T. Ho and C.S. Yang, 1992. Inhibitory Effect of Oral Administration of Green Tea on Tumorigenesis by Utraviolet Light, 12-0-tetradecanoylphorbol-13-acetate and N-nitrosodiethylamine in Mice. In: Cancer Chemoprevention- Lee, W., L. Martin, B.W., Charles and K. Gary. (Eds.), CRC Press Inc., New York, pp: 362.
8. Das, B.K., S.C. Mukherjee, B.B. Sahu and G. Murjani, 1999. Neem ( Azadirachta indica) extracts as an antibacterial agent against fish pathogenic bacteria. Indian J. Exp. Biol., 37: 1097-1100.
9. Erdélyi, M., M. Mézes and G. Virág, 1999. Selenium dependent glutathione peroxidases in animals. I. Structure, function, regulation (in Hungarian). Biokémia, 23: 82-88.
10. Et-shazly, M.M. and E.D. Et-sharnoub, 2000. Toxicity of a Neem ( Azadirachta indica) insecticide to certain aquatic organisms. J. Egyptian Soc. Parasitol., 30: 221-231.
11. Habig, W.H., M.J. Pabst and W.B. Jacob, 1974. Glutathione S-transferase. The first enzymatic step in mercapturic acid formation. J. Biol. Chem., 249: 7130-7139.
12. Habig, W,H. and W.B. Jakoby, 1981. Assays for differentiation of glutathione S-transferases. Methods Enzymol., 77: 398-405.
13. Hall, G.D., P.M. Patel and A.S. Protheroe, 1998. Key Topics in Oncology, Selby, P.J. (Edn.), United Kingdom: Bios Scientific Publishers.
14. Hesham, M.S., H.M. Mostafa, P.J. O'Connor, J.A. Rafferty and J.D. Michael, 2002. Evidence for the presence of active cytochrome P-450 systems in Schistosoma mansoni and Schistosoma haematobium adult worms. FEBS Left., 519: 205-209.
15. Hubert, E.B., 2003. Molecular therapy and prevention of HCC. http://www.hccl.htm/ Accessed on 14 May 2003.
16. Gordon, M.C. and J.N. David, 2005. Plants as a source of anti-cancer agents. J. Ethnoharmacol., 100: 72-79.
17. Kew, M.C., 2000. Hepatocellular carcinoma. A century of progress. Clin. Liver Dis., 4: 257-268.
18. Lam, L.K.T., J. Zhang, S. Hasegawa and H.A.J. Schut, 1994. Enzymes Changes in Cancer Treated in vivo Model. In: Food Phytochemicals for Cancer Prevention 1: Fruits and Vegetables, Edition Ho, C.T., T. Osawa, M.T. and R.T. Rosen (Eds.). Washington: American Chemical Society.
19. Lawrence, R.A. and C. Burk, 1976. Glutathione peroxidase activity in selenium deficient rat liver. Biochem. Biophys. Res. Commun., 71: 952-958.
20. Lysandro, P.B., C.B. Vanessa, V. Angelica, N. Cristina, B. Joao and G.Z. Rocha, 2005. Protective effect of diphenyl diselenide on acute liver damage induced by 2-nitropropane in rats, Toxicology, 210-1, 1-8.
21. Majno, P., G. Emiliano, M. Philippe, H. Antoine and M. Gilles, 2005, Management of hepatocellular carcinoma in the waiting list before liver transplantation. J. Hepatol., 42: S134-S143.
22. Man-Fung, Y. and L. Ching-Lung, 2005. Serological markers of liver cancer. Best Practice Res. Clin. Gastroenterol., 19: 91-99.
23. Marrs, K.A., 1996. Function and regulation of glutathione S-transferase in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol., 47: 127.
24. Masi, S.D., M.E. Tosti and A. Mele, 2005. Screening for hepatocellular carcinoma. Digestive and Liver Dis., 37: 260-268.
25. Parida, M.M., C. Upadhyay, G. Pandya and A.M. Jana, 2002. Inhibitory potential of neem (Azadirachta indica Juss) leaves on Dengue virus type-2 replication. J. Ethnopharmacol., 79: 273-278.
26. Pongtip, S., S. Roongtawan and G. Wandee, 2005. Antioxidant activity of Siamese neem tree (VP1209). J. Ethnopharmacol., 99: 109-112.
27. 1-mediated hepatocellular carcinoma, Fitoterapia, 70-3-1, 279-283.
28. Sacande, M., J. Buitink and F.A. Hoekstra, 2000. A study of water relatiom in neem (Azadiracheta indica) seed that is characterized by complex storage behaviour. J. Exp. Bot., 51: 635-643.
29. Sai Ram, M., G. Ilavazhagan, S.K. Sharma, S.A. Dhanraj, B. Suresh, M.M. Parida, A.M. Jana, K. Devendra and W. Selvamurthy, 2000. Antimicrobial activity of a new vaginal contraceptive NIM-76 from neem oil ( Azadirachta indica). J. Ethnophamacol., 71: 377-382.
30. Sala, M., J. Fuster, J.M. Llovet, M. Navasa, M. Sole and M. Varela, 2004. Barcelona Clinic Liver Cancer (BCLC) Group High pathological risk of recurrence after surgical resection for hepatocellular carcinoma: An indication for salvage liver transplantation. Liver Transplat., 10: 1294-1300.
31. Subapriya, R. and S. Nagini, 2005. Medicinal properties of neem leaves: A review. Cu. Med. Chem. Anticancer Agents, 5: 149-146.
32. Tartarinov, Y. S., 1964. Detection of embryo-specific a-globulin in the blood serum of a patient with primary cancer of the liver. Med. Khim. SSSR, 10: 90-91.
33. Yu, F., S. Lu, F. Yu, S. Feng, P.M. McGuire, R. Li and R. Wang, 2006. Protective effects of polysaccharide from Euphorbia kansui (Euphorbiaceae) on the swimming exercise-induced oxidative stress in mice. Can. J. Physiol. Pharmacol., 84: 1071-1079.