The patch clamp technique: Principles and technical considerations

Journal of Veterinary Cardiology

Volumn 9, Issue 1, 2007, Pages 25-37

  Kornreich, Bruce G ^a  

^a Department of Obstetrics Gynecology   (United States)

Author keywords

Electrophysiology; Ion channels; Patch clamp

Indexed keywords

ION CHANNEL;

ARTICLE; BLOOD VESSEL CAPACITANCE; CARDIOVASCULAR DISEASE; CELL ISOLATION; CELL MEMBRANE; CHANNEL GATING; DIAGNOSTIC PROCEDURE; ELECTROCARDIOGRAPHY; ELECTRODE; HEART ELECTROPHYSIOLOGY; HEART MUSCLE CELL; INTRACELLULAR RECORDING; ION CONDUCTANCE; ION CURRENT; MATHEMATICAL COMPUTING; MEASUREMENT; NONHUMAN; PATCH CLAMP; PATHOPHYSIOLOGY; PATIENT CARE; PRIORITY JOURNAL; PROGNOSIS; VOLTAGE CLAMP;

ANIMALS; ELECTROPHYSIOLOGY; HEART; ION CHANNELS; MEMBRANE POTENTIALS; PATCH-CLAMP TECHNIQUES;

EID: 34249093627     PISSN: 17602734     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jvc.2007.02.001     Document Type: Article

References (10)

1. Hodgkin A.L., and Huxley A.F. A quantitative description of ion channels and its application to conduction and excitation in nerve membranes. J Phys 117 (1952) 500-544
2. Neher E., and Sakmann B. Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature 260 5554 (1976) 799-802
3. Hamill O.P., Marty A., Neher E., et al. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch 391 2 (1981) 85-100
4. Sachs F., and Auerbach A. Single-channel electrophysiology: use of the patch clamp. Meth Enzymol 103 (1983) 147-176
5. Wellis D.P., DeFelice L.J., and Mazzanti M. Outward sodium current in beating heart cells. Biophys J 57 1 (1990) 41-48
6. Mazzanti M., and DeFelice L.J. Ca channel gating during cardiac action potentials. Biophys J 58 4 (1990) 1059-1065
7. Antzelevitch C., Pollevick G.D., Cordeiro J.M., et al. Loss-of-function mutations in the cardiac calcium channel underlie a new clinical entity characterized by ST-segment elevation, short QT intervals, and sudden cardiac death. Circulation 115 4 (2007) 442-449
8. Antzelevitch C., Sicouri S., Litovsky S.H., et al. Heterogeneity within the ventricular wall. Electrophysiology and pharmacology of epicardial, endocardial, and M cells. Circ Res 69 6 (1991) 1427-1449
9. Hille B. Ionic selectivity, saturation, and block in sodium channels. A four-barrier model. J Gen Physiol 66 5 (1975) 535-560
10. Zheng J., Vankataramanan L., and Sigworth F.J. Hidden Markov model analysis of intermediate gating steps associated with the pore gate of shaker potassium channels. J Gen Physiol 118 5 (2001) 547-564