Localization of EPS components secreted by freshwater diatoms using differential staining with fluorophore-conjugated lectins and other fluorochromes

European Journal of Phycology

Volumn 42, Issue 2, 2007, Pages 199-208

  Bahulikar, Rahul A ^a   Kroth, Peter G ^a  

^a UNIVERSITY OF KONSTANZ   (Germany)

Author keywords

DAPI (diamidino phenylindole); DTAF (dichlorotriazinyl aminofluorescein); Epilithic biofilm; Lake Constance; Lectins

Indexed keywords

BIOCHEMICAL COMPOSITION; BIOFILM; CARBOHYDRATE; CHEMICAL BINDING; DEVELOPMENTAL STAGE; DIATOM; DYE TRACER; EXOPOLYMER; FRESHWATER ECOSYSTEM; INTERTIDAL ENVIRONMENT; PROTEIN; SECRETION; ULTRASTRUCTURE;

CENTRAL EUROPE; EURASIA; EUROPE; LAKE CONSTANCE;

ACHNANTHES; ASTERIONELLA; BACILLARIOPHYTA; CALONEIS ALPESTRIS; CYMBELLA; DIATOMA; FRAGILARIA; GOMPHONEMA; MELOSIRA; STAUROSIRA; SYNEDRA;

EID: 34249017933     PISSN: 09670262     EISSN: 14694433     Source Type: Journal    
DOI: 10.1080/09670260701289779     Document Type: Article

References (35)

1. CHIOVITTI, A., BACIC, A., BURKE, J. & WETHERBEE, R. (2003a). Heterogeneous xylose-rich glycans are associated with extracellular glycoproteins from the biofouling diatom Craspedosauros australis (Bacillariophyceae). Eur. J. Phycol., 38: 351-360.
2. CHIOVITTI, A., HIGGINS, M.J., HARPER, R.E., WETHERBEE, R. & BACIC, A. (2003b). The complex polysaccharides of the raphid diatom Pinnularia viridis (Bacillariophyceae). J. Phycol., 39: 543-554.
3. COX, E.J. (1981). Mucilage tube morphology of three tube-dwelling diatoms and its diagnostic value. J. Phycol., 17: 72-80.
4. DANIEL, G.F., CHAMBERLAIN, A.H.L. & JONES, E.B.G. (1987). Cytochemical and electron microscopical observations on the adhesive materials of marine fouling diatoms. Br. Phycol. J., 22: 101-118.
5. DAWSON, P.A. (1973). Observations on some species of the diatom genus Gomphonema C. A. Agardh. Br. Phycol. J., 8: 413-423.
6. DE BROUWER, J.F.C. & STAL, L.J. (2001). Short-term dynamics in microphytobenthos distribution and associated extracellular carbohydrates in surface sediments of an intertidal mudflat. Mar. Ecol. Prog. Ser., 218: 33-44.
7. DE BROUWER, J.F.C. & STAL, L.J. (2002). Daily fluctuations of exopolymers in cultures of the benthic diatoms Cylindrotheca closterium and Nitzschia sp. (Bacillariophyceae). J. Phycol., 38: 464-472.
8. DE BROUWER, J.F.C., WOLFSTEIN, K. & STAL, L.J. (2002). Physical characterization and diel dynamics of different fractions of extracellular polysaccharides in an axenic culture of a benthic diatom. Eur. J. Phycol., 37: 37-44.
9. HECKY, R.E., MOPPER, K., KILHAM, P. & DEGENS, E.T. (1973). The amino acid and sugar composition of diatom cell-walls. Mar. Biol., 19: 323-331.
10. HIGGINS, M.J., CRAWFORD, R.M., MULVANEY, P. & WETHERBEE, R. (2000). The topology of soft, adhesive diatom 'trails' as observed by atomic force microscopy. Biofouling, 16: 133-139.
11. HIGGINS, M.J., CRAWFORD, S.A., MULVANEY, P. & WETHERBEE, R. (2002). Characterization of the adhesive mucilages secreted by live diatom cells using atomic force microscopy. Protist, 153: 25-38.
12. HOAGLAND, K.D., ROSOWSKI, J.R., GRETZ, M.R. & ROEMER, S.C. (1993). Diatom extracellular polymeric substances: function, fine structure, chemistry, and physiology. J. Phycol., 29: 537-566.
13. HUNTSMAN, S.A. & SLONEKER, J.H. (1971). An exocellular polysaccharide from the diatom Gomphonema olivaceum. J. Phycol., 7: 261-264.
14. KAWAHARASAKI, M., TANAKA, H., KANAGAWA, T. & NAKAMURA, K. (1999). In situ identification of polyphosphate-accumulating bacteria in activated sludge by dual staining with rRNA-targeted oligonucleotide probes and 4′,6-diamidino-2-phenylindol (DAPI) at a polyphosphate-probing concentration. Water Res., 33: 257-265.
15. LIND, J.L., HEIMANN, K., MILLER, E.A., VAN VLIET, C., HOOGENRAAD, N.J. & WETHERBEE, R. (1997). Substratum adhesion and gliding in a diatom are mediated by extracellular proteoglycans. Planta, 203: 213-221.
16. PERKINS, R.G., UNDERWOOD, G.J.C., BROTAS, V., SNOW, G.C., JESUS, B. & RIBEIRO, L. (2001). Responses of microphytobenthos to light: primary production and carbohydrate allocation over an emersion period. Mar. Ecol. Prog. Ser., 223: 101-112.
17. RHODES, L.L. (1998). Identification of potentially toxic Pseudo-Nitzschia (Bacillariophyceae) in New Zealand coastal waters, using lectins. New Zeal. J. Mar. Fresh., 32: 537-544.
18. ROSOWSKI, J.R., ROEMER, S.C., PALMER, J. & HOAGLAND, K.D. (1986). Extracellular association and adaptive significance of the bas-relief pad of Achnanthes lanceolata (Bacillariophyceae). Diatom Res., 1: 113-129.
19. SCHUMANN, R. & RENTSCH, D. (1998). Staining particulate organic matter with DTAI- a fluorescence dye for carbohydrates and protein: a new approach and application of a 2D image analysis system. Mar. Ecol. Prog. Ser., 163: 77-88.
20. SMESTAD-PAULSEN, B., HAUG, A. & LARSEN, B. (1978). Structural studies of a carbohydrate-containing polymer present in the mucilage tubes of the diatom Berkeleya rutilans (Trent.) Grun. Carbohydr. Res., 66: 103-111.
21. SONG, S.-C., CHEN, Y.-Y., HERP, A., TSAI, M.-S. & WU, A.M. (1999). Lectinochemical characterization of a GalNAc and multi-Gal β1→4GlcNAc reactive lectin from Wistaria sinensis seeds. Eur. J. Biochem., 266: 778-788.
22. STAATS, N., DE WINDER, B., STAL, L.J. & MUR, L.R. (1999). Isolation and characterization of extracellular polysaccharides from epipelic diatoms Cylindrotheca closterium and Navicula salinarum. Eur. J. Phycol., 34: 161-169.
23. STAATS, N., STAL, L.J. & MUR, L.R. (2000). Exopolysaccharide production by the epipelic diatom Cylindrotheca closterium: effects of nutrient conditions. J. Exp. Mar. Biol. Ecol., 249: 13-27.
24. STAL, L.J. & DÉFARGE, C. (2005). Structure and dynamics of exopolymers in an intertidal diatoms biofilm. Geomicrobiol. J., 22: 341-352.
25. STAL, L.J. & DE BROUWER, J.F.C. (2003). Biofilm formation by benthic diatoms and their influence on the stabilization of intertidal mudflats. Berichte-Forschungszentrum TERRAMARE, 12: 109-111.
26. UNDERWOOD, G.J.C. & SMITH, D.J. (1998). Predicting epipelic diatom exopolymer concentration in intertidal sediments from sediment chlorophyll a. Microb. Ecol., 35: 116-125.
27. WANG, Y., CHEN, Y., LAVIN, C. & GRETZ, M.R. (2000). Extracellular matrix assembly in diatoms (Bacillariophyceae). IV. Ultrastructure of Achnanthes longipes and Cymbella cistula as revealed by high-pressure freezing/freeze substitution and cryo-field emission scanning electron microscopy. J. Phycol., 36: 367-378.
28. WATANABE, M.M. (2005). Freshwater culture media. In Algal Culturing Techniques (Anderson, R.A., editor), 13-20. Elsevier Academic Press, London.
29. WATT, W.D. (1969). Extracellular release of organic matter from two freshwater diatoms. Ann. Bot., 33: 427-437.
30. WETHERBEE, R., LIND, J.L., BURKE, J. & QUATRANO, R.S. (1998). The first kiss: establishment and control of initial adhesion by raphid diatoms. J. Phycol., 34: 9-15.
31. WHITE, R.J. & CHAMBERLAIN, A.H.L. (1982). Attachment of epiphytic freshwater diatoms. Microsc., 34: 470-476.
32. WIGGLESWORTH-COOKSEY, B. & COOKSEY, K.E. (2005). Use of flurophore-conjugated lectins to study cell-cell interactions in model marine biofilms. Appl. Environ. Microbiol., 71: 428-435.
33. WOLFSTEIN, K. & STAL, L.J. (2002). Production of extracellular polymeric substances (EPS) by benthic diatoms: effect of irradiance and temperature. Mar. Ecol. Prog. Ser., 236: 13-22.
34. WUSTMAN, B.A., CRETZ, M.R. & HOAGLAND, K.D. (1997). Extracellular matrix assembly in diatoms (Bacillariophyceae). Plant Physiol., 113: 1059-1069.
35. YALLOP, M.L., PATERSON, D.M. & WELLBURY, P. (2000). Inter-relationships between rates of microbial production, exopolymer production, microbial biomass, and sediment stability in biofilms of intertidal sediments. Microb. Ecol., 39: 116-127.