Endothelial cell density in porcine corneas after exposure to hypotonic solutions

Graefe's Archive for Clinical and Experimental Ophthalmology

Volumn 245, Issue 1, 2007, Pages 143-147

  Meltendorf, Christian ^a   Ohrloff, Christian ^a   Rieck, Peter ^b   Schroeter, Jan ^b  

^a GOETHE UNIVERSITY   (Germany)

^b CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Corneal endothelium; Eye banking; Organ culture; Osmotic stress; Sucrose

Indexed keywords

ALIZARIN; BALANCED SALT SOLUTION; DEXTRAN; HYPOTONIC SOLUTION; SUCROSE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL DENSITY; CELL LOSS; CONTROLLED STUDY; CORNEA EPITHELIUM; CULTURE MEDIUM; EXPOSURE; NONHUMAN; OPTIC DISK; ORGAN CULTURE; PRIORITY JOURNAL; RETINA IMAGE; STAINING; STATISTICAL ANALYSIS; TISSUE CULTURE;

ACETATES; ANIMALS; ANTHRAQUINONES; CELL COUNT; DEXTRANS; DRUG COMBINATIONS; ENDOTHELIUM, CORNEAL; HYPOTONIC SOLUTIONS; MINERALS; ORGAN CULTURE TECHNIQUES; OSMOTIC PRESSURE; SODIUM CHLORIDE; SUCROSE; SWINE;

EID: 34248667598     PISSN: 0721832X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00417-006-0334-2     Document Type: Article

References (20)

1. Armitage WJ, Easty DL (1997) Factors influencing the suitability of organ-cultured corneas for transplantation. Invest Ophthalmol Vis Sci 38:16-24
2. Armitage WJ, Moss SJ, Easty DL (1988) Effects of osmotic stress on rabbit corneal endothelium. Cryobiology 25:425-439
3. Bahn CF, Glassman RM, MacCallum DK, Lillie JH, Meyer RF, Robinson BJ et al. (1986) Postnatal development of corneal endothelium. Invest Ophthalmol Vis Sci 27:44-51
4. Borderie VM, Baudrimont M, Lopez M, Carvajal S, Laroche L (1997) Evaluation of the deswelling period in dextran-containing medium after corneal organ culture. Cornea 16:215-223
5. Edelhauser HF, Hanneken AM, Pederson HJ, Van Horn DL (1981) Osmotic tolerance of rabbit and human corneal endothelium. Arch Ophthalmol 99:1281-1287
6. Edelhauser HF, Van Horn DL, Schultz RO, Hyndiuk RA (1976) Comparative toxicity of intraocular irrigating solutions on the corneal endothelium. Am J Ophthalmol 81:473-481
7. Gonnering R, Edelhauser HF, Van Horn DL, Durant W (1979) The pH tolerance of rabbit and human corneal endothelium. Invest Ophthalmol Vis Sci 18:373-390
8. Hagenah M, Bohnke M (1993) Latent endothelial cell damage after experimental corneal cryopreservation. Graefe's Arch Clin Exp Ophthalmol 231:529-532
9. Maas-Reijs J, Pels E, Tullo AB (1997) Eye banking in Europe 1991-1995. Acta Ophthalmol Scand 75:541-543
10. Pegg DE, Hunt CJ, Fong LP (1987) Osmotic properties of the rabbit corneal endothelium and their relevance to cryopreservation. Cell Biophys 10:169-189
11. Pels E, Schuchard Y (1984) The effects of high molecular weight dextran on the preservation of human corneas. Cornea 3:219-227
12. Pels E, Schuchard Y (1983) Organ-culture preservation of human corneas. Doc Ophthalmol 56:147-153
13. Redbrake C, Salla S, Nilius R, Becker J, Reim M (1997) A histochemical study of the distribution of dextran 500 in human corneas during organ culture. Curr Eye Res 16:405-411
14. Singh G, Bohnke M, Draeger J (1984) Endothelial wound healing in organ-cultured pig corneae after mechanical trauma. Fortschr Ophthalmol 81:293-295
15. Singh G, Bohnke M, von-Domarus D, Draeger J, Lindstrom RL, Doughman DJ (1985) Vital staining of corneal endothelium. Cornea 4:80-91
16. Spence DJ, Peyman GA (1976) A new technique for the vital staining of the corneal endothelium. Invest Ophthalmol 15:1000-1002
17. Sperling S (1985) Assessment of endothelial cell density in bovine corneas after osmotically induced dilation of intercellular spaces. Cornea 4:71-79
18. Sperling S (1986) Evaluation of the endothelium of human donor corneas by induced dilation of intercellular spaces and trypan blue. Graefe's Arch Clin Exp Ophthalmol 224:428-434
19. Sperling S, Gundersen HJ (1978) The precision of unbiased estimates of numerical density of endothelial cells in donor cornea. Acta Ophthalmol (Copenh) 56:793-802
20. Taylor MJ, Hunt CJ (1981) Dual staining of corneal endothelium with trypan blue and alizarin red S: Importance of pH for the dye-lake reaction. Br J Ophthalmol 65:815-819