A high-throughput fluorimetric assay for angiotensin I-converting enzyme

Nature Protocols

Volumn 1, Issue 4, 2006, Pages 1961-1964

  Schwager, Sylva L ^a   Carmona, Adriana K ^b   Sturrock, Edward D ^a  

^a UNIVERSITY OF CAPE TOWN   (South Africa)

^b FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; DIPEPTIDYL CARBOXYPEPTIDASE; HIPPURYLHISTIDYLLEUCINE; HISTIDYLLEUCINE; REAGENT; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CLINICAL EFFECTIVENESS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME KINETICS; ENZYME SUBSTRATE; FLUORESCENCE ANALYSIS; FLUOROMETRY; HIGH THROUGHPUT SCREENING; INTERMETHOD COMPARISON; PRIORITY JOURNAL;

PEPTIDYL-DIPEPTIDASE A; SPECTROMETRY, FLUORESCENCE;

EID: 34248656190     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2006.305     Document Type: Article

References (14)

1. Costabel, U. & Teschler, H. Biochemical changes in sarcoidosis. Clin. Chest Med. 18, 827-842 (1997).
2. Baudin, B. New aspects on angiotensin-converting enzyme: From gene to disease. Clin. Chem. Lab. Med. 40, 256-265 (2002).
3. Skeggs, S.L.T. Jr, Kahn, J.R. & Shumway, N.P. The preparation and function of the hypertensin-converting enzyme. J. Exp. Med. 103 295-299 (1956).
4. Helmer, O.M. Differentiation between two forms of angiotonin by means of spirally cut strips of rabbit aorta. Am. J. Physiol. 188, 571-577 (1957).
5. Mehanna, A.S. & Dowling, M. Liquid chromatographic determination of hippuric acid for the evaluation of ethacrynic acid as angiotensin converting enzyme inhibitor. J. Pharmaceut. Biomed. Anal. 19 967-973 (1999).
6. Wu, J., Aluko, R.E. & Muir, A.D. Improved method for direct high-performance liquid chromatography assay of angiotensin-converting enzyme-catalyzed reactions. J. Chromatogr. A 950, 125-130 (2002).
7. Xiao, X., Luo, X., Chen, B. & Yao, S. Determination of angiotensin converting enzyme inhibitory activity by high-performance liquid chromatography/ electrospray-mass spectrometry. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 834, 48-54 (2006).
8. Cushman, D.W. & Cheung, H.S. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem. Pharmacol. 20, 1637-1648 (1971).
9. Soffer, R.L. Angiotensin-converting enzyme and the regulation of vasoactive peptides. Annu. Rev. Biochem. 45, 73-94 (1976).
10. Piquilloud, Y., Reinharz, A. & Roth, M. Action of converting enzyme on synthetic substrates. Helv. Physiol. Pharmacol. Acta 26, CR-2 (1968).
11. Piquilloud, Y., Reinharz, A. & Roth, M. Studies on the angiotensin converting enzyme with different substrates. Biochim. Biophys. Acta 206, 136-142 (1970).
12. Friedland, J. & Silverstein, E. A sensitive fluorimetric assay for seruman giotensin-converting enzyme. Am. J. Clin. Pathol. 66 416-424 (1976).
13. Bunning, P. & Riordan, J.F. Activation of angiotensin converting enzyme by monovalent anions. Biochemistry 22, 110-116 (1983).
14. Wei, L., Clauser, E., Alhenc-Gelas, F. & Corvol, P. The two homologous domains of human angiotensin I-converting enzyme interact differently with competitive inhibitors. J. Biol. Chem. 267, 13398-13405 (1992).