In situ hybridization technique for mRNA detection in whole mount Arabidopsis samples

Nature Protocols

Volumn 1, Issue 4, 2006, Pages 1939-1946

  Hejátko, Jan ^a   Blilou, Ikram ^b   Brewer, Philip B ^c   Friml, Jiří ^a,c   Scheres, Ben ^b   Benková, Eva ^c  

^a MASARYK UNIVERSITY   (Czech Republic)

^b UTRECHT UNIVERSITY   (Netherlands)

^c UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA;

ARABIDOPSIS; ARTICLE; AUTOMATION; GENE EXPRESSION; HIGH THROUGHPUT SCREENING; IN SITU HYBRIDIZATION; MICROARRAY ANALYSIS; NONHUMAN; PLANT GENETICS; PLANT GROWTH; PLANT LEAF; PLANT SEED; PRIORITY JOURNAL; PROTEIN LOCALIZATION; ROBOTICS; SIGNAL DETECTION; TISSUE DISTRIBUTION;

ARABIDOPSIS; GENE EXPRESSION REGULATION, PLANT; IN SITU HYBRIDIZATION; RNA, ANTISENSE; RNA, MESSENGER; TISSUE FIXATION;

ARABIDOPSIS;

EID: 34248636482     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2006.333     Document Type: Article

References (14)

1. Tautz, D. & Pfeifle, C. A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback. Chromosoma 98, 81-85 (1989).
2. de Almeid Engler, J., Van Montagu, M. & Engler, G. Whole-mount in situ hybridization in plants. Methods Mol. Biol. 82, 373-384 (1998).
3. Bennett, M.J. et al. Arabidopsis AUX1 gene: A permease-like regulator of root gravitropism. Science 273, 948-950 (1996).
4. Ludevid, D., Hofte, H., Himelblau, E. & Chrispeels, M.J. The expression pattern of the tonoplast intrinsic protein gamma-TIP in Arabidopsis thaliana is correlated with cell enlargement. Plant Physiol. 100, 1633-1639 (1992).
5. Plickert, G., Gajewski, M., Gehrke, G., Gausepohl, H., Schlossherr, J. & Ibrahim, H. Automated in situ detection (AISD) of biomolecules. Dev. Genes Evol. 207, 362-367 (1997).
6. Nonradioactive In Situ Hybridisation Application Manual, 2nd edition, 51-56 (Boehringer Manheim 1996).
7. Friml, J., Benková, E., Mayer, U., Palme, K. & Muster, G. Automated whole-mount localization techniques for plant seedlings. Plant J. 34, 115-124 (2003).
8. García-Aguilar, M., Dorantes-Acosta, A., Pérez-España, V. & Vielle-Calzada, J.P. Whole-mount in situ mRNA localization in developing ovules and seeds of Arabidopsis. Plant Mol. Biol. Reporter 23, 279-289 (2005).
9. Zachgo, S., Perbal, M.C., Saedler, H. & Schwarz-Sommer, Z. In situ analysis of RNA and protein expression in whole mounts facilitates detection of floral gene expression dynamics. Plant J. 23, 697-702 (2000).
10. Fisher, H.W. & Williams, R.C. Electron microscopic visualization of nucleic acids and of their complexes with proteins. Annu. Rev. Biochem. 48, 649-679 (1979).
11. Malamy, J.E. & Benfey, P.N. Organization and cell differentiation in lateral roots of Arabidopsis thaliana. Development, 124, 33-44 (1997).
12. Hamann, T., Benková, E., Bäurle, I., Kientz, M. & Jürgens, G. The Arabidopsis BODENLOS gene encodes an auxin response protein inhibiting MONOPTEROS- mediated embryo patterning. Genes & Dev. 16, 1610-1615 (2002).
13. Friml, J. et al. AtPIN4 mediates sink driven auxin gradients and patterning in Arabidopsis roots. Cell, 108, 661-673 (2002).
14. Blilou, I. et al. The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots. Nature, 433, 39-44 (2005).