An improved synthesis of NHS-MAG3 for conjugation and radiolabeling of biomolecules with 99mTc at room temperature

Nature Protocols

Volumn 2, Issue 4, 2007, Pages 972-978

  Wang, Yi ^a   Liu, Xinrong ^a   Hnatowich, Donald J ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BOVINE SERUM ALBUMIN; MERTIATIDE TC 99M; N HYDROXYSUCCINIMIDYL S ACETYLMERCAPTOACETYLTRIGLYCINIC ACID; N SUCCINIMIDYL S ACETYLMERCAPTOACETIC ACID; RADIOPHARMACEUTICAL AGENT; TECHNETIUM 99M; THIOGLYCOLIC ACID; THIOGLYCOLIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; COMPLEX FORMATION; CONJUGATION; DRUG PURIFICATION; DRUG QUALITY; DRUG STRUCTURE; DRUG SYNTHESIS; GEL PERMEATION CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH TEMPERATURE; ISOTOPE LABELING; PRIORITY JOURNAL; QUALITY CONTROL; REACTION ANALYSIS; ROOM TEMPERATURE; TEMPERATURE SENSITIVITY;

CHELATING AGENTS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ISOTOPE LABELING; OLIGOPEPTIDES; ORGANOTECHNETIUM COMPOUNDS; SERUM ALBUMIN; SERUM ALBUMIN, BOVINE; SUCCINIMIDES; SULFIDES; TECHNETIUM; TEMPERATURE;

BOVINAE;

EID: 34248582937     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.144     Document Type: Article

References (14)

1. Hnatowich, D.J. et al. Labeling peptides with technetium-99m using a bifunctional chelator of a N-hydroxysuccinimide ester of mercaptoacetyltriglycine. J. Nucl. Med. 39, 56-64 (1998).
2. 188Re-radiolabeled phosphorodiamidate morpholino oligomer, a synthetic DNA analogue. Clin. Cancer Res. 12, 4958-4964 (2006).
3. 3 and SHNH: An in vitro and animal in vivo comparison. Nucl. Med. Biol. 23, 917-922 (1996).
4. 3 and DTPA) on tumor cell accumulation and mouse biodistribution of technetium-99m labeled to antisense DNA. Eur. J. Nucl. Med. 27, 1700-1707 (2000).
5. 3-annexin V: influence of the chelate on in vitro and in vivo properties in mice. Nucl. Med. Biol. 33, 135-144 (2006).
6. 99mTc-labeling and biodistribution of a CAPL antisense oligodeoxynucleotide. Nucl. Med. Biol. 25, 651-657 (1998).
7. Fritzberg, A.R., Kasina, S., Eshima, D. & Johnson, D.L. Synthesis and biological evaluation oftechnetium-99m MAG3 as a hippuran replacement. J. Nucl. Med. 27, 111-116 (1986).
8. 99Tc-MAG3-Biotin conjugates for antibody pretargeting strategies. Nucl. Med. Biol. 25, 611-619 (1998).
9. Okarvi, S.M. & Jammaz, I.A. Preparation and in vitro and in vivo evaluation of technetium-99m-labeled folate and methotrexate conjugates as tumor imaging agents. Cancer Biother. Radiopharm. 21, 49-60 (2006).
10. Rusckowski, M., Gupta, S., Liu, G., Dou, S. & Hnatowich, D.J. Investigations of a Tc-99m-labeled bacteriophage as a potential infection-specific imaging agent. J. Nucl. Med. 45, 1201-1208 (2004).
11. Duncan, R.J.S., Weston, P.D. & Wrigglesworth, R. A new reagent which may be used to introduce sulfhydryl-groups into proteins, and its use in the preparation of conjugates for immunoassay. Anal. Biochem. 132 68-73 (1983).
12. 3]octreotide. Bioconjugate Chem. 10, 431-438 (1999).
13. 99mTc radiolabeling of biomolecules. Nat. Protocols 1, 1477-1480 (2006).
14. 3 for technetium-99m labeling of DNA. Nucl. Med. Biol. 24, 425-432 (1997).