Yeast two-hybrid screening

Methods in Molecular Biology

Volumn 362, Issue , 2007, Pages 207-223

  Maple, Jodi ^a   Møller, Simon G ^a  

^a NONE

Author keywords

Auxotrophy; Bait protein; cDNA library; Diploids; GAL4 activation domain; GAL4 DNA binding domain; LacZ; MATCHMAKER system; Prey protein; Protein expression; Reporter gene; Yeast mating; Yeast transformation; Yeast two hybrid

Indexed keywords

GAL4 PROTEIN, S CEREVISIAE; HYBRID PROTEIN; RECOMBINANT DNA; SACCHAROMYCES CEREVISIAE PROTEIN; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ARTICLE; CHEMISTRY; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; METABOLISM; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; REPORTER GENE; SACCHAROMYCES CEREVISIAE; TWO HYBRID SYSTEM;

BASE SEQUENCE; DNA, RECOMBINANT; GENES, REPORTER; GENETIC VECTORS; PROTEIN INTERACTION MAPPING; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSCRIPTION FACTORS; TRANSFORMATION, GENETIC; TWO-HYBRID SYSTEM TECHNIQUES;

EID: 34248547649     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1385/1-59745-257-2:207     Document Type: Article

References (6)

1. Fields, S., and Song O. K. (1989) A novel genetic system to detect protein-protein interactions. Nature 340, 245-246.
2. Chien, C. T., Bartel, P. L., Sternglanz, R., and Fields, S. (1991) The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proc. Natl. Acad. Sci. USA 88, 9578-9582.
3. Sambrook, J., and Russell, D. W. (2001) Molecular Cloning: A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
4. Birnboim, H. C., and Doly, J. (1979) A rapid alkaline procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1, 1513-1523.
5. Clontech Yeast Protocols Handbook, PT3024-1 (2001). BD BioSciences, Clontech, Palo Alto, CA.
6. Hengen, P. N. (1997) False positives from the yeast two-hybrid system. Trends Biochem. Sci. 11, 33-34.