Extraction of plant RNA

Methods in Molecular Biology

Volumn 362, Issue , 2007, Pages 309-314

  Salter, Michael G ^a   Conlon, Helen E ^a  

^a NONE

Author keywords

Acid phenol method; Arabidopsis thaliana; Efficient; Limited tissue; Total RNA extraction

Indexed keywords

ARABIDOPSIS; ARABIDOPSIS THALIANA; EMBRYOPHYTA;

ACETIC ACID; ALCOHOL; PHENOL; PLANT RNA;

ARABIDOPSIS; ARTICLE; CHEMISTRY; DNA MICROARRAY; GENETICS; ISOLATION AND PURIFICATION; NORTHERN BLOTTING; PRECIPITATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ARABIDOPSIS; BLOTTING, NORTHERN; ETHANOL; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PHENOL; PRECIPITATION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, PLANT; SODIUM ACETATE;

EID: 34248547645     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1385/1-59745-257-2:309     Document Type: Article

References (8)

1. Verwoerd,T. C., Dekker,B. M. M.,and Hoekema, A. (1989) A small scale procedure for rapid isolation of plant RNA's. Nucleic Acids Res. 17, 6, 2362.
2. Salter, M. G., Franklin, K. A., and Whitelam, G. C. (2003) Gating of the rapid shade avoidance response by the circadian clock in plants. Nature 426, 680-683.
3. Groppe, J. C., and Morse, D. E. (1993). Isolation of full length RNA templates for reverse transcription from tissues rich in RNAse and proteoglycans. Ann. Biochem. 210, 337-343.
4. Perry, R. P., Torre, J. L., Kelly, D. E., and Greenberg, J. R. (1972) On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes. Biochim. Biophys. Acta 14, 262 (2):220-226.
5. Brawerman, G., Mendecki, J., and Lee, S. Y. (1972) A procedure for the isolation of mammalian messenger ribonucleic acid. Biochemistry 11(4):637-641.
6. Ausübel, F. A., Brent, R., Kingston, R. E., et al. (1987) Current Protocols in Molecular Biology. John Wiley and Sons.
7. Wallace, D. M. (1987) Large- and small-scale phenol extractions. Methods Enzymol. 152, 33-41.
8. Smabrook, J. and Russell, D. W. (2001) Molecular Cloning: A. Laboratory Manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.