RNA in situ hybridizations on Drosophila whole mounts

Methods in Molecular Biology

Volumn 362, Issue , 2007, Pages 495-511

  Wülbeck, Corinna ^a   Helfrich Förster, Charlotte ^a  

^a NONE

Author keywords

Alkaline phosphatase; Dig POD; DIG UTP; Drosophila; Fixation; Fluorescence; Hapten; Horseradish peroxidase; Primary antibody; Riboprobe; RNA in situ hybridization; Secondary antibody; Signal amplification; Transcript; Whole mounts

Indexed keywords

ARMORACIA RUSTICANA;

ANTIBODY; DNA; FLUORESCENT DYE; HAPTEN; PROTEINASE K; RNA;

ANIMAL; ARTICLE; BRAIN; CHRONOBIOLOGY; CONFOCAL MICROSCOPY; DROSOPHILA MELANOGASTER; FLUORESCENCE IN SITU HYBRIDIZATION; GENE; GENE VECTOR; GENETIC TRANSCRIPTION; GENETICS; IN SITU HYBRIDIZATION; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; RNA PROBE;

ANIMALS; ANTIBODIES; BASE SEQUENCE; BRAIN; CHRONOBIOLOGY; DNA; DROSOPHILA MELANOGASTER; ENDOPEPTIDASE K; FLUORESCENT DYES; GENES, INSECT; GENETIC VECTORS; HAPTENS; IN SITU HYBRIDIZATION; IN SITU HYBRIDIZATION, FLUORESCENCE; MICROSCOPY, CONFOCAL; MOLECULAR SEQUENCE DATA; RNA; RNA PROBES; TRANSCRIPTION, GENETIC;

EID: 34248545580     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1385/1-59745-257-2:495     Document Type: Article

References (12)

1. Tautz, D., and Pfeifle, C. (1989) A non radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals a translational control of the segmentation gene hunchback. Chromosoma 98, 81-85.
2. Lehmann, R., and Tautz, D. (1994) In situ hybridization to RNA. In: Methods in Cell Biology (Goldstein, L. S. B. and Fyrberg, E. A., eds.). San Diego, CA, Academic Press, pp. 575-598.
3. Hauptmann, G., and Gerster, T. (1994) Two color whole mount in situ hybridization to vertebrates and Drosophila embryos. Trends Genet. 10, 266.
4. Cohen, B., and. Cohen, S. M. (1992) Double labelling of mRNA and proteins in Drosophila embryos. In: Nonradioactive Labelling and Detection of Biomolecules (Kessler, C., ed.), Springer-Verlag, Berlin/Heidelberg, pp. 382-392.
5. Pattatucci, A., and Kaufman, T. (1992) Antibody staining of imaginal discs. DIS 71, 147.
6. Wülbeck, C., and Simpson, P. (2000) Expression of achaete-scute homologues in discrete proneural clusters of the developing notum of the medfly Ceratitis capitata suggests a common origin for the stereotyped bristle pattern of higher Diptera. Development 127, 1411-1420.
7. Wülbeck, C., and Simpson, P. (2002) The expression of pannier and achaetescute homologues in a mosquito suggests an ancient role of pannier as a selector gene in the regulation of the dorsal body pattern. Development 169, 3861-3871.
8. Hughes, S. C., and Krause, H. M. (1998) Single and double FISH protocols for Drosophila. In: Confocal Microscopy. Methods and Protocols (Paddock, S. W., ed.). Humana, Totowa, NJ, pp. 93-101.
9. Nagaso, H., Murara, T., Day, N., and Yokoyama, K. K. (2001) Simultaneous detection of RNA and protein by in situ hybridization and immunological staining. J. Histochem. Cytochem. 49, 1177-1182.
10. O'Neill, J. W., and Bier, E. (1994) Double-label in situ hybridization using biotin and digoxigenin tagged RNA probes. Biotechniques 17, 870-875.
11. Wilkie, G. S., and Davies, I. (1998) Visualizing mRNA by in situ hybridization using high resolution and sensitive tyramide signal amplification. Technical Tips Online, T01458.
12. Denkers, N., Garcia-Villalba, P., Rodesch, C. K., Nielson, K. R., and Mauch, T.J. (2004) FISHing for chick genes: triple-label whole-mount fluorescence in situ hybridization detects simultaneous and overlapping gene expression in avian embryos. Dev. Dyn. 229, 651-657.