Dual-reporter assay using two secreted luciferase genes

BioTechniques

Volumn 42, Issue 3, 2007, Pages 290-292

  Wu, Chun ^a   Suzuki Ogoh, Chie ^a   Ohmiya, Yoshihiro ^a,b  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b HOKKAIDO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

2 METHYL 6 PHENYL 3,7 DIHYDROIMIDAZO(1,2 A)PYRAZIN 3 ONE; 2-METHYL-6-PHENYL-3,7-DIHYDROIMIDAZO(1,2-A)PYRAZIN-3-ONE; LUCIFERASE; LUCIFERIN; PYRAZINE DERIVATIVE; UNCLASSIFIED DRUG;

ANIMAL; ARTICLE; BIOTECHNOLOGY; DOSE RESPONSE; FIREFLY; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; LUMINESCENCE; METABOLISM; METHODOLOGY; PLASMID; REPORTER GENE; TIME;

ANIMALS; BIOTECHNOLOGY; DOSE-RESPONSE RELATIONSHIP, DRUG; FIREFLIES; FIREFLY LUCIFERIN; GENES, REPORTER; GENETIC VECTORS; LUCIFERASES; LUMINESCENCE; LUMINESCENT MEASUREMENTS; PLASMIDS; PYRAZINES; TIME FACTORS; TRANSFECTION;

EID: 34248531946     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112428     Document Type: Article

References (8)

1. Ohmiya, Y. 2005. Basic and applied aspects of color tuning of bioluminescence systems. Jpn. J. Appl. Phys. 22:1543-1545.
2. Naylor, L.-H. 1999. Reporter gene technology: the future looks bright. Biochem. Pharmacol. 58:749-757.
3. Nakajima, Y., T. Kimura, K. Sugata, T. Enomoto, T. Asakawa, H. Kubota, M. Ikeda, and Y. Ohmiya. 2005. A multicolor luciferase assay system, one-step monitoring of multiple gene expressions with a single substrate. BioTechniques 38:891-894.
4. Nakajima, Y., K. Kobayashi, K. Yamagishi, T. Enomoto, and Y. Ohmiya. 2004. cDNA cloning and characterization of a secreted luciferase from the luminous Japan ostracod, Cypridina noctiluca. Biosci. Biotechnol. Biochem. 68:565-570.
5. Verhaegent, M. and T.K. Christopoulos. 2002. Recombinant Gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization. Anal. Chem. 74:4378-4385.
6. Inouye, S., Y. Ohmiya, Y. Toya, and F.I. Tsuji. 1992. Imaging of luciferase secretion from transformed Chinese hamster ovary cells. Proc. Natl. Acad. Sci. USA 89:9584-9587.
7. Wu, C. K. Kawasaki, S. Ohgiya, and Y. Ohmiya. 2006. Syntheses and evaluation of the bioluminescent activity of (S)-Cypridina luciferin and its analogs, Tetrahedron Lett. 47:753-756.
8. Yamagishi, K., T. Enomoto, and Y. Ohmiya. 2006. Perfusion-culture-based secreted bioluminescence reporter assay in living cells. Anal. Biochem. 354:15-21.