Evaluation of a new pooling strategy based on leukocyte count for rapid quantification of allele frequencies

Clinical Chemistry

Volumn 53, Issue 5, 2007, Pages 980-982

  Rossmann, Heidi ^a   Büchler, Elena ^a   Wenzel, Jürgen J ^a   Neukirch, Carolin ^a   Du Prel, Jean Baptist ^a   Lackner, Karl J ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BLOOD SAMPLING; DNA EXTRACTION; DNA SEQUENCE; GENE FREQUENCY; GENOTYPE; HUMAN; LEUKOCYTE COUNT; QUANTITATIVE ANALYSIS; RELIABILITY; THAWING;

AUTOANALYSIS; DNA; GENE FREQUENCY; HUMANS; LEUKOCYTE COUNT; LEUKOCYTES; POLYMORPHISM, SINGLE NUCLEOTIDE;

EID: 34248388004     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2006.083691     Document Type: Article

References (5)

1. Sham P, Bader JS, Craig I, O'Donovan M, Owen M. DNA pooling: a tool for large-scale association studies. [Review] Nat Rev Genet 2002;3:862-71.
2. Alderborn A, Kristofferson A, Hammerling U. Determination of single-nucleotide polymorphisms by real-time pyrophosphate DNA sequencing. Genome Res 2000;10:1249-58.
3. Neve B, Froguel P, Corset L, Vaillant E, Vatin V, Boutin P. Rapid SNP allele frequency determination in genomic DNA pools by pyrosequencing. Biotechniques 2002;2:1138-42.
4. Shifman S, Pisante-Shalom A, Yakir B, Darvasi A. Quantitative technologies for allele frequency estimation of SNPs in DNA pools. Mol Cell Probes 2002;16:429-34.
5. Lavebratt C, Sengul S, Jansson M, Schalling M. Pyrosequencing-based SNP allele frequency estimation in DNA pools. Hum Mutat 2004;23:92-7.