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In general, only qualitative trends can be made, as the collision energy is not well defined in CID MS/MS when using quadrupole ion trap instruments (ref 22a). Although it is possible to obtain quantitative gas phase dissociation energies using mass spectrometry e.g., Hammad, L. A.; Gerdes, G.; Chen, P. Organometallics 2005, 24, 1907;
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In general, only qualitative trends can be made, as the collision energy is not well defined in CID MS/MS when using quadrupole ion trap instruments (ref 22a). Although it is possible to obtain quantitative gas phase dissociation energies using mass spectrometry (e.g., Hammad, L. A.; Gerdes, G.; Chen, P. Organometallics 2005, 24, 1907;
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36
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85153551393
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The normalized collision energy (E) is a standardized collision energy scale based on the amplitude of the applied resonance excitation if voltage (used for inducing fragmentation) and the mass of the parent ion. The amplitude of the rf voltage is given by (E/0.3)(me + a), where m (u) is the parent mass and a (V) and b (V/u) are instrument-dependent parameters (tick amp intercept and slope). This is a useful quantity, as it normalizes out the differences between instruments and the parent mass effect. More details can be found in: Lopez, L. L.; Tiller, P. R.; Senko, M. W.; Schwartz, J. C. Rapid Commun. Mass Spectrom. 1999, 13, 663.
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The normalized collision energy (E) is a standardized collision energy scale based on the amplitude of the applied resonance excitation if voltage (used for inducing fragmentation) and the mass of the parent ion. The amplitude of the rf voltage is given by (E/0.3)(me + a), where m (u) is the parent mass and a (V) and b (V/u) are instrument-dependent parameters (tick amp intercept and slope). This is a useful quantity, as it normalizes out the differences between instruments and the parent mass effect. More details can be found in: Lopez, L. L.; Tiller, P. R.; Senko, M. W.; Schwartz, J. C. Rapid Commun. Mass Spectrom. 1999, 13, 663.
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