Comparison of an enrichment broth-enhanced commercial PCR procedure versus bacteriological culture for separating non-colonized from suspected or colonized MRSA individuals

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 26, Issue 3, 2007, Pages 155-160

  Wagenvoort, J H T ^a   Van De Cruijs, M F H A ^a   Meuwissen, C T M ^a   Gronenschild, J M H ^a   De Brauwer, E I G B ^a  

^a Sittard Geleen Heerlen   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

PENICILLIN BINDING PROTEIN 2A;

ARTICLE; BACTERIAL INFECTION; BACTERIUM CULTURE; COAGULASE NEGATIVE STAPHYLOCOCCUS; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; EPIDEMIC; GENE; HOSPITAL LABORATORY; HUMAN; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NETHERLANDS; POLYMERASE CHAIN REACTION; PREVALENCE; PRIORITY JOURNAL; SAMPLE; STAPHYLOCOCCUS AUREUS;

EID: 34248233073     PISSN: 09349723     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10096-007-0269-5     Document Type: Article

References (14)

1. Wagenvoort JHT (2000) Dutch measures to control MRSA and the expanding European Union. Eurosurveillance 5:26-28
2. Dutch Working Party on Infection Prevention (2005) Policy for methicillin-resistant Staphylococcus aureus (in Dutch). http://www.wip.nl
3. Wagenvoort JHT, De Brauwer EIGB, Sijstermans MLH, Toenbreker HMJ (2005) Risk of re-introduction of methicillin-resistant Staphylococcus aureus into the hospital by intrafamilial spread from and to healthcare workers. J Hosp Infect 59:67-76
4. Wagenvoort JHT, De Brauwer E, Toenbreker H, Heck M, Wannet W (2002) Endemic MRSA shakes the Dutch "search and destroy" strategy. Brit Med J 324:240b, http://bmj.com/cgi/eletters/324/7331/240/b
5. Hagen RM, Seegmuller I, Navai J, Kappstein I, Lehn N, Miethke T (2005) Development of real-time PCR assay for rapid identification of methicillin-resistant Staphylococcus aureus from clinical samples. Int J Med Microbiol 295:77-86
6. Nilsson P, Alexandersson H, Ripa T (2005) Use of broth enrichment and real-time PCR to exclude the presence of methicillin-resistant Staphylococcus aureus in clinical samples: a sensitive screening approach. Clin Microbiol Infect 11:1027-1034
7. Warren DK, Liao RS, Merz LR, Eveland M, Dunne WM Jr (2004) Detection of methicillin-resistant Staphylococcus aureus directly from nasal swab specimens by a real-time PCR assay. J Clin Microbiol 42:5578-5581
8. Huletsky A, Lebel P, Picard FJ, Bernier M, Gagnon M, Bocher N, Bergeron MG (2005) Identification of methicillin-resistant Staphylococcus aureus carriage in less than 1 hour during a hospital surveillance program. Clin Infect Dis 40:976-981
9. Kaiser P, Reischl U, Miethke T, Lehn N, Schneider-Brachert W (2005) Comparison of two different real-time PCR-assays for rapid identification of MRSA directly from clinical swabs. In: Program and abstracts of the 45th ICCAC™ Washington. Abstract no. D-1714
10. Brown DF, Edwards DI, Hawkey PM, Morrison D, Ridgway GL, Towner KJ, Wren MW (2005) Guidelines for the laboratory diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus (MRSA). On behalf of the joint working party of the British Society for Antimicrobial Chemotherapy, Hospital Infection Society and Infection Control Nurses Association. J Antimicrob Chemother 56:1000-1018
11. Wagenvoort JHT, Werink TJ, Gronenschild JMH, Davies BI (1996) Optimization of detection of methicillin-resistant Staphylococcus aureus phage type III-29. Infect Control Hosp Epidemiol 17:208-209
12. Robert Koch Institute (2004) Zur MRSA-Situationen in Deutschland im Jahr 2004. Epidemiol Bull 4:376-380
13. Becker K, Pagnier I, Schuhen B, Wenzelburger F, Friedrich AW, Kipp F, Peters G, von Eiff C (2005) Does nasal colonization by methicillin-resistant coaugulase-negative staphylococci and methicillin-susceptible Staphylococcus aureus strains occur frequently enough to represent a risk of false-positive methicillin-resistant S. aureus determinations by molecular methods? J Clin Microbiol 44:229-231
14. Daeschlein G, Assadian O, Daxboeck F, Kramer A (2006) Multiplex PCR-Elisa for direct detection of MRSA in nasal swabs advantageous for rapid identification of non-MRSA carriers. Eur J Clin Microbiol Infect Dis 25:328-330