Experiments linking nitrogenase gene expression to nitrogen fixation in the North Pacific subtropical gyre

Limnology and Oceanography

Volumn 52, Issue 1, 2007, Pages 169-183

  Zehr, Jonathan P ^a   Montoya, Joseph P ^b   Jenkins, Bethany D ^a   Hewson, Ian ^a   Mondragon, Elizabeth ^a   Short, Cindy M ^a   Church, Matthew J ^c   Hansen, Andrew ^c   Karl, David M ^c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

^c UNIVERSITY OF HAWAII   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ABUNDANCE; BACTERIUM; GENE EXPRESSION; GROWTH; GYRE; MICROBIAL ACTIVITY; NITROGEN FIXATION; POLYMERASE CHAIN REACTION; SUBTROPICAL REGION;

HAWAII [UNITED STATES]; HAWAIIAN ISLANDS; KANEOHE BAY; NORTH AMERICA; OAHU; PACIFIC ISLANDS; PACIFIC OCEAN; UNITED STATES;

JOINVILLEA ASCENDENS;

EID: 34248225462     PISSN: 00243590     EISSN: None     Source Type: Journal    
DOI: 10.4319/lo.2007.52.1.0169     Document Type: Article

References (38)

1. BIRD, C., J. MARTINEZ MARTINEZ, A. G. O'DONNELL, AND M. WYMAN. 2005. Spatial distribution and transcriptional activity of an uncultured clade of planktonic diazotrophic γ-Proteobacteria in the Arabian Sea. Appl. Environ. Microbiol. 71: 2079-2085.
2. CHEN, Y.-B., B. DOMINIC, M. T. MELLON, AND J. P. ZEHR. 1998. Circadian rhythm of nitrogenase gene expression in the diazotrophic filamentous nonheterocystous cyanobacterium Trichodesmium sp. Strain IMS 101. J. Bacteriol. 180: 3598-3605.
3. CHURCH, M. J., B. D. JENKINS, D. M. KARL, AND J. P. ZEHR. 2005a. Vertical distributions of nitrogen-fixing phylotypes at Station ALOHA in the oligotrophic North Pacific Ocean. Aquat. Microb. Ecol. 38: 3-14.
4. _, C. SHORT, D. M. KARL, AND J. P. ZEHR. 2005b. Temporal patterns of nitrogenase (nifH) gene expression in the oligotrophic North Pacific Ocean. Appl. Environ. Microbiol. 71: 5362-5370.
5. COLÓN-LÓPEZ, M., D. M. SHERMAN, AND L. A. SHERMAN. 1997. Transcriptional and translational regulation of nitrogenase in light-dark-and-continuous-light crown cultures of the unicellular cyanobacterium Cyanothece sp. Strain ATCC 51142. J. Bacterid. 179: 4319-4327.
6. DORE, J. E., J. R. BRUM, L. M. TUPAS, AND D. M. KARL. 2002. Seasonal and interannual variability in sources of nitrogen supporting export in the oligotrophic subtropical North Pacific Ocean. Limnol. Oceanogr. 47: 1595-1607.
7. DYHRMAN, S. T., P. D. CHAPPELL, S. T. HALEY, J. W. MOFFETT, E. D. ORCHARD, J. B. WATERBURY, AND E. A. WEBB. 2006. Phosphonate utilization by the globally important marine diazotroph Trichodesmium. Nature 439: 68-71.
8. _, AND S. T. HALEY. 2006. Phosphorus scavenging in the unicellular marine diazotroph Crocosphaera watsonii. Appl. Environ. Microbiol. 72: 1452-1458.
9. 2 fixation by unicellular bacterioplankton from the Atlantic and Pacific Oceans: Phylogeny and in situ rates. Appl. Environ. Microbiol. 70: 765-770.
10. 2 fixing cyanobacteria from the tropical North Atlantic and subtropical North Pacific Oceans. J. Phycol. 40: 1074-1078.
11. GRUBER, N. 2005. Oceanography - a bigger nitrogen fix. Nature 436: 786-787.
12. HESS, W. R., G. ROCAP, C. S. TING, F. LARIMER, S. STILWAGEN, J. LAMERDIN, AND S. W. CHISHOLM. 2001. The photosynthetic apparatus of Prochlorococcus: Insights through comparative genomics. Photosynthesis Res. 70: 53-71.
13. KARL, D. M. 2002. Nutrient dynamics in the deep blue sea. Trends Microbiol. 10: 410-418.
14. _, AND OTHERS. 2001. Ecological nitrogen-to-phosphorus stoichiometry at Station ALOHA. Deep-Sea Res. Part II-Topical Studies in Oceanography 48: 1529-1566.
15. KLAUSMEIER, C. A., E. LITCHMAN, T. DAUFRESNE, AND S. A. LEVIN. 2004. Optimal nitrogen-to-phosphorus stoichiometry of phytoplankton. Nature 429: 171-174.
16. LUDWIG, W., AND OTHERS. 2004. ARB: a software environment for sequence data. Nucleic Acids Research 32: 1363-1371.
17. MAZARD, S. L., N. J. FULLER, K. M. ORCUTT, O. BRIDLE, AND D. J. SCANLAN. 2004. PCR analysis of the distribution of unicellular cyanobacterial diazotrophs in the Arabian Sea. Appl. Environ. Microbiol. 70: 7355-7364.
18. MILLS, M. M., C. RIDAME, M. DAVEY, J. LA ROCHE, AND R. J. GEIDER. 2004. Iron and phosphorus co-limit nitrogen fixation in the eastern tropical North Atlantic. Nature 429: 292-294.
19. MITSUI, A., S. CAO, A. TAKAHASHI, AND T. ARAL 1987. Growth synchrony and cellular-parameters of the unicellular nitrogen-fixing marine cyanobacterium, synechococcus sp strain miami Bg 043511 under continuous illumination. Physiol. Plant. 69: 1-8.
20. 2 fixation by unicellular diazotrophs in the oligotrophic Pacific Ocean. Nature 430: 1027-1032.
21. 2 fixation. Appl. Environ. Microbiol. 62: 986-993.
22. ORCHARD, E., E. WEBB, AND S. DYHRMAN. 2003. Characterization of phosphorus-regulated genes in Trichodesmium spp. Biol Bull 205: 230-231.
23. ORTEGACALVO, J. J., AND L. J. STAL. 1991. Diazotrophic growth of the unicellular cyanobacterium Gloeothece sp PCC 6909 in continuous culture. J Gen Microbiol 137: 1789-1797.
24. PARTENSKY, F., W. R. HESS, AND D. VAULOT. 1999. Prochlorococcus, a marine photosynthetic prokaryote of global significance. Microbiol Mol Biol Rev 63: 106-127.
25. REDFIELD, A. C. 1958. The biological control of chemical factors in the environment. Am Sci 46: 205-222.
26. ROCAP, G., AND OTHERS. 2003. Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation. Nature 424: 1042-1047.
27. SAÑUDO-WILHELMY, S. A., AND OTHERS. 2001. Phosphorus limitation of nitrogen fixation by Trichodesmium in the central Atlantic Ocean. Nature 411: 66-69.
28. SCHNEEGURT, M. A., D. M. SHERMAN, S. NAYAR, AND L. A. SHERMAN. 1994. Oscillating behavior of carbohydrate granule formation and dinitrogen fixation in the cyanobacterium Cyanothece sp. strain ATCC 51142. J. Bacteriol. 176: 1586-1597.
29. SHORT, S. M., B. D. JENKINS, AND J. P. ZEHR. 2004. Spatial and temporal distribution of two diazotrophic bacteria in the Chesapeake Bay. Appl. Environ. Microbiol. 70: 2186-2192.
30. STAL, L. J. 2003. Smart modelling of unusual cyanobacteria - an enigma solved? New Phytol 160: 455-457.
31. THOMPSON, J. R., AND OTHERS. 2005. Genotypic diversity within a natural coastal bacterioplankton population. Science 307: 1311-1313.
32. TILLETT, D., AND B. A. NEILAN. 2000. Xanthogenate nucleic acid isolation from cultured and environmental cyanobacteria. J. Phycol. 36: 251-258.
33. TYRRELL, T. 1999. The relative influences of nitrogen and phosphorus on oceanic primary production. Nature 400: 525-531.
34. ZEHR, J. P., L. L. CRUMBLISS, M. J. CHURCH, E. O. OMOREGIE, AND B. D. JENKINS. 2003a. Nitrogenase genes in PCR and RT-PCR reagents: implications for studies of diversity of functional genes. Biotechniques 35: 996-1005.
35. _, B. D. JENKINS, S. M. SHORT, AND G. F. STEWARD. 2003b. Nitrogenase gene diversity and microbial community structure: a cross-system comparison. Environ. Microbiol. 5: 539-554.
36. _, AND L. A. MCREYNOLDS. 1989. Use of degenerate oligonucleotides for amplification of the nifH gene from the marine cyanobacterium Trichodesmium spp. Appl. Environ. Microbiol. 55: 2522-2526.
37. _, M. T. MELLON, AND S. ZANI. 1998. New nitrogen fixing microorganisms detected in oligotrophic oceans by the amplification of nitrogenase (nifH) genes. Appl. Environ. Microbiol. 64: 3444-3450.
38. 2 in the subtropical North Pacific Ocean. Nature 412: 635-638.