mBAND analysis of chromosomal aberrations in human epithelial cells exposed to low- and high-LET radiation

Radiation Research

Volumn 168, Issue 1, 2007, Pages 98-105

  Hada, Megumi ^a,b   Cucinotta, Francis A ^b   Gonda, Steve R ^b   Wu, Honglu ^b  

^a UNIVERSITIES SPACE RESEARCH ASSOCIATION   (United States)

^b NASA JOHNSON SPACE CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MARKER; ION; IRON;

ARTICLE; CHROMOSOME 3; CHROMOSOME ABERRATION; CHROMOSOME ANALYSIS; EPITHELIUM CELL; GAMMA RADIATION; HUMAN; HUMAN CELL; IN SITU HYBRIDIZATION; IN VITRO STUDY; PREMATURE CHROMOSOME CONDENSATION; PRIORITY JOURNAL; RADIATION EXPOSURE; RADIATION INJURY; CELL LINE; COLOR; LINEAR ENERGY TRANSFER; METABOLISM;

CELL LINE; CHROMOSOME ABERRATIONS; COLOR; EPITHELIAL CELLS; HUMANS; IN SITU HYBRIDIZATION; LINEAR ENERGY TRANSFER;

EID: 34248169114     PISSN: 00337587     EISSN: None     Source Type: Journal    
DOI: 10.1667/RR0759.1     Document Type: Article

References (42)

1. T. C. Yang, K. George, A. S. Johnson, M. Durante and B. S. Fedorenko, Biodosimetry results from space flight Mir-18. Radiat. Res. 148 (Suppl.), S17-S23 (1997).
2. G. Obe, I. Johannes, C. Johannes, K. Hallman, G. Reitz and R. Facius, Chromosomal aberrations in blood lymphocytes of astronauts after long-term space flights. Int. J. Radiat. Biol. 72, 727-734 (1997).
3. K. George, M. Durante, H. Wu, V. Willingham, G. Badhwar and F. A. Cucinotta, Chromosome aberrations in the blood lymphocytes of astronauts after space flight. Radiat. Res. 156, 731-738 (2001).
4. M. Durante, S. Bonassi, K. George and F. A. Cucinotta, Risk estimation based on chromosomal aberrations induced by radiation. Radiat. Res. 156, 662-667 (2001).
5. NCRP, Guidance on Radiation Received in Space Activities. Report No. 98, National Council on Radiation Protection and Measurements, Bethesda, MD, 1989.
6. D. J. Brenner and R. K. Sachs, Chromosomal "fingerprints" of prior exposure to densely ionizing radiation. Radiat. Res. 140, 134-142 (1994).
7. T. Cremer and C. Cremer, Chromosome territories, nuclear architecture and gene regulation in mammalian cells. Nat. Rev. Genet. 2, 292-301 (2001).
8. M. P. Hande, T. V. Azizova, C. R. Geard, L. E. Burak, C. R. Mitchell, V. F. Khokhryakov, E. K. Vasilenko and D. J. Brenner, Past exposure to densely ionizing radiation leaves a unique permanent signature in the genome. Am. J. Hum. Genet. 72, 1162-1170 (2003).
9. F. Hutchinson, Analysis of deletions induced in the genome of mammalian cells by ionizing radiation. J. Mol. Biol. 254, 372-380 (1995).
10. H. Wu, K. George, V. Willingham, T. Kawata and F. A. Cucinotta, Comparison of F ratios generated from interphase and metaphase chromosome damage induced by high doses of low- and high-LET radiation. Radiat. Res. 155, 57-62 (2001).
11. M. Bauchinger and E. Schmid, Is there reliable experimental evidence for a chromosomal "fingerprint" of exposure to densely ionizing radiation? Radiat. Res. 147, 506-510 (1997).
12. N. Nakamura, J. D. Tucker, M. Bauchinger, L. G. Littlefield, D. C. Lloyd, R. J. Preston, M. S. Sasaki, A. A. Awa and S. Wolff, F values as cytogenetic fingerprints of prior exposure to different radiation qualities: Prediction, reality and future. Radiat. Res. 150, 492-494 (1998).
13. C. Johannes, M. Horstmann, M. Durante, I. Chudoba and G. Obe, Chromosome intrachanges and interchanges detected by multicolor banding in lymphocytes: Searching for clastogen signatures in the human genome. Radiat. Res. 161, 540-548 (2004).
14. E. J. Tawn and C. A. Whitehouse, Chromosome intra- and interchanges determined by G-banding in radiation workers with in vivo exposure to plutonium. J. Radiol. Prot. 25, 83-88 (2005).
15. H. Wu, M. Durante, Y. Furusawa, K. George, T. Kawata and F. A. Cucinotta, Truly incomplete and complex exchanges in prematurely condensed chromosomes of human fibroblasts exposed in vitro to energetic heavy ions. Radiat. Res. 160, 418-424 (2003).
16. J. N. Lucas, W. Deng, S. W. Oram, F. S. Hill, M. Durante, K. George, H. Wu, C. L. Owens and T. Yang, Theoretical and experimental tests of a chromosomal fingerprint for densely ionizing radiation based on F ratios calculated from stable and unstable chromosome aberrations. Radiat. Res. 151, 85-91 (1999).
17. R. Karhu, M. Ahlstedt-Soini, M. Bittner, P. Meltzer, J. M. Trent and J. J. Isola, Chromosome arm-specific multicolor FISH. Genes Chromosomes Cancer 30, 105-109 (2001).
18. I. Chudoba, A. Plesch, T. Lorch, J. Lemke, U. Claussen and G. Senger, High resolution multicolor-banding: a new technique for refined FISH analysis of human chromosomes. Cytogenet. Cell Genet. 84, 156-160 (1999).
19. J. Y. Ostashevsky, Higher-order structure of interphase chromosomes and radiation-induced chromosomal exchange aberrations. Int. J. Radiat. Biol. 76, 1179-1187 (2000).
20. M. R. Stampfer and J. C. Bartley, Induction of transformation and continuous cell lines from normal human mammary epithelial cells after exposure to benzo(a)pyrene. Proc. Natl. Acad. Sci. USA 82, 2394-2398 (1985).
21. T. C. Yang and L. M. Craise, Development of human epithelial cell systems for radiation risk assessment. Adv. Space Res. 14, 115-120 (1994).
22. T. C. Yang, L. M. Craise, J. C. Prioleau, M. R. Stampfer and J. S. Rhim, Chromosomal changes in cultured human epithelial cells transformed by low- and high-LET radiation. Adv. Space Res. 12, 127-136 (1992).
23. T. C. Yang, K. A. Georgy, A. Tavakoli, L. M. Craise and M. Durante, Radiogenic transformation of human mammary epithelial cells in vitro. Radiat. Oncol. Investig. 3, 412-419 (1996).
24. T. C. Yang, K. A. George, M. Mei, M. Durante and L. M. Craise, Radiogenic cell transformation and carcinogenesis. ASGSB Bull. 2, 106-112 (1995).
25. T. C. Yang, K. A. George, L. M. Craise and M. Durante, Initiation of oncogenic transformation in human mammary epithelial cells by charged particles. Radiat. Oncol. Investig. 5, 134-138 (1997).
26. S. R. Gonda, H. Wu, P. L. Pingerelli and B. W. Glickman, Three-dimensional transgenic cell model to quantify genotoxic effects of space environment. Adv. Space Res. 27, 421-430 (2001).
27. T. C. Yang, M. R. Stampfer and C. A. Tobias, Radiation studies on sensitivity and repair of human mammary epithelial cells. Int. J. Radiat. Biol. 56, 605-609 (1989).
28. M. Durante, G. F. Grossi, G. Gialanella, M. Pugliese, M. Nappo and T. C. Yang, Effects of α-particles on survival and chromosomal aberrations in human mammary epithelial cells. Radiat. Environ. Biophys. 34, 195-205 (1995).
29. A. Lehnert, E. Lessmann, J. Pawelke and W. Dörr, RBE of 25 kV X-rays for the survival and induction of micronuclei in the human mammary epithelial cell line MCF-12A. Radiat. Environ. Biophys. 45, 253-260 (2006).
30. M. Durante, G. F. Grossi and T. C. Yang, Radiation-induced chromosomal instability in human mammary epithelial cells. Adv. Space Res. 18, 99-108 (1996).
31. 2-phase block and repair of radiation-induced chromosome fragments in human lymphocytes. Radiat. Res. 151, 670-676 (1999).
32. R. M. Anderson, S. J. Marsden, E. G. Wright, M. A. Kadhim, D. T. Goodhead and C. S. Griffin, Complex chromosome aberrations in peripheral blood lymphocytes as a potential biomarker of exposure to high-LET alpha-particles. Int. J. Radiat. Biol. 76, 31-42 (2000).
33. R. M. Anderson, S. J. Marsden, S. J. Paice, A. E. Bristow, M. A. Kadhim, C. S. Griffin and D. T. Goodhead, Transmissible and non-transmissible complex chromosome aberrations characterized by three-color and mFISH define a biomarker of exposure to high-LET α particles. Radiat. Res. 159, 40-48 (2003); Erratum, Radiat. Res. 159, 437 (2003).
34. S. Ritter, E. Nasonova, E. Gudowska-Nowak, M. Scholz and G. Kraft, High-LET-induced chromosome aberrations in V79 cells analysed in first and second post-irradiation metaphases. Int. J. Radiat. Biol. 76, 149-161 (2000).
35. K. George, M. Durante, V. Willingham, H. Wu, T. C. Yang and F. A. Cucinotta, Biological effectiveness of accelerated particles for the induction of chromosome damage measured in metaphase and interphase human lymphocytes. Radiat. Res. 160, 425-435 (2003).
36. C. R. Mitchell, T. V. Azizova, M. P. Hande, L. E. Burak, J. M. Tsakok, V. F. Khokhryakov, C. R. Geard and D. J. Brenner, Stable intrachromosomal biomarkers of past exposure to densely ionizing radiation in several chromosomes of exposed individuals. Radiat. Res. 162, 257-263 (2004).
37. R. K. Sachs, A. M. Chen and D. J. Brenner, Proximity effects in the production of chromosome aberrations by ionizing radiation. Int. J. Radiat. Biol. 71, 1-19 (1997).
38. M. Durante, K. George, H. Wu and F. A. Cucinotta, Karyotypes of human lymphocytes exposed to high-energy iron ions. Radiat. Res. 158, 581-590 (2002).
39. R. M. Anderson, D. L. Stevens and D. T. Goodhead, M-FISH analysis shows that complex chromosome aberrations induced by alpha-particle tracks are cumulative products of localized rearrangements. Proc. Natl. Acad. Sci. USA 99, 12167-12172 (2002).
40. M. P. Hande, T. V. Azizova, L. E. Burak, V. F. Khokhryakov, C. R. Geard and D. J. Brenner, Complex chromosome aberrations persist in individuals many years after occupational exposure to densely ionizing radiation: An mFISH study. Genes Chromosomes Cancer 44, 1-9 (2005).
41. E. J. Tawn, C. A. Whitehouse and A. E. Riddell, FISH chromosome analysis of plutonium workers from the Sellafield Nuclear Facility. Radiat. Res. 165, 592-597 (2006).
42. M. Hortsmann, M. Durante, C. Johannes and G. Obe, Chromosomal intrachanges induced by swift iron ions. Adv. Space Res. 35, 276-279 (2005).