Enhance the efficiency of single-strand conformation polymorphism analysis by short polyacrylamide gel and modified silver staining

Analytical Biochemistry

Volumn 365, Issue 2, 2007, Pages 286-287

  Zhang, ChunLei ^a,b   Wang, Yanhong ^a   Chen, Hong ^a,c   Lan, XianYong ^a,d   Lei, ChuZhao ^a  

^a NORTHWEST A F UNIVERSITY   (China)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^c JIANGSU NORMAL UNIVERSITY   (China)

^d ZHEJIANG SCI TECH UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

SILVER;

POLYACRYLAMIDE GELS; SILVER STAINING; SINGLE-STRAND CONFORMATION POLYMORPHISM;

POLYMORPHISM;

POLYACRYLAMIDE GEL; SINGLE STRANDED DNA;

ARTICLE; EXON; GENE AMPLIFICATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SILVER STAINING; SINGLE STRAND CONFORMATION POLYMORPHISM;

EID: 34248163596     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.03.023     Document Type: Article

References (5)

1. Humphries S.E., Gudnason V., Whittall R., and Day I.N.M. Single-strand conformation polymorphism analysis with high throughput modifications, and its use in mutation detection in familial hypercholesterolemia. Clin. Chem. 43 (1997) 427-435
2. Pierluigi S., Silvia S., Renato E., Gian P.B., Giovanni B., and Guido B. Cold single-strand conformation polymorphism analysis: Optimization for detection of APC gene mutations in patients with familial adenomatous polyposis. Intl. J. Mol. Med. 8 (2001) 567-572
3. Kajiwara H., and Kaneko T. Utility of single-strand conformation polymorphism analysis was improved by low-cross-linking polyacrylamide gel and quick low-background silver staining. Anal. Biochem. 300 (2002) 273-274
4. Bassam B.J., Caetano-Anolles G., and Gresshoff P.M. Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal. Biochem. 195 (1991) 80-83
5. In: Sambrook J., and Russell D.W. (Eds). Molecular Cloning: A Laboratory Manual vol. 13 (2000), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 49-56