Employing the fluorescence anisotropy and quenching kinetics of tryptophan to hunt for residual structures in denatured proteins

Journal of Chemical Sciences

Volumn 119, Issue 2, 2007, Pages 141-145

  Kumar, Satish ^a   Swaminathan, Rajaram ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY GUWAHATI   (India)

Author keywords

Guanidine hydrochloride; Hydrophobia cluster; Indole; Iodide; Polarization; Protein folding

Indexed keywords

FLUORESCENCE QUENCHING; HUMAN SERUM ALBUMIN; INDOLE; IODIDES; MELITTIN; RIBONUCLEASE; TRYPTOPHAN;

CONFORMATIONS; DENATURATION; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; IODINE COMPOUNDS; LIGHT POLARIZATION; NUCLEAR MAGNETIC RESONANCE; OPTICAL ANISOTROPY; PROTEIN FOLDING;

AMINO ACIDS;

EID: 34248139272     PISSN: 02534134     EISSN: 09737103     Source Type: Journal    
DOI: 10.1007/s12039-007-0021-9     Document Type: Article

References (25)

1. Hammarstrom P and Carlsson U 2000 Biochem. Biophys. Res. Commun. 276 393
2. Dill K A and Shortle D 1991 Annu. Rev. Biochem. 60 795
3. Neri D, Billeter M, Wider G and Wuthrich K 1992 Science 257 1559
4. Tafer H, Hiller S, Hilty C, Fernandez C and Wuthrich K 2004 Biochemistry 43 860
5. Pearce M C, Cabrita L D, Rubin H, Gore M G and Bottomley S P 2004 Biochem. Biophys. Res. Commun. 324 729
6. Crowhurst K A, Tollinger M and Forman-Kay J D 2002 J. Mol. Biol. 322 163
7. Klein-Seetharaman J, Oikawa M, Grimshaw S B, Wirmer J, Duchardt E, Ueda T, Imoto T, Smith L J, Dobson C M and Schwalbe H 2002 Science 295 1719
8. Evans P A, Topping K D, Woolfson D N and Dobson C M 1991 Proteins: Struct. Funct. Genet. 9 248
9. Ropson I J and Freiden C 1992 Proc. Natl. Acad. Sci. USA 89 7222
10. Lakowicz J R (ed.) 2000 Topics in fluorescence spectroscopy (London: Kluwer Academic/Plenum) vol. 6
11. Beechem J M and Brand L 1985 Annu. Rev. Biochem. 54 43
12. Eftink M R 1991 Fluorescence quenching: Theory and applications. In Topics in fluorescence spectroscopy (ed.) J R Lakowicz (New York: Plenum) vol. 2, pp 53-126
13. Lakshmikanth G S and Krishnamoorthy G 1999 Biophys. J. 77 1100
14. Garcia P, Merola F, Receveur V, Blandin P, Minard P and Desmadril M 1998 Biochemistry 37 7444
15. Steiner R F 1991 Fluorescence anisotropy: Theory and application. In Topics of fluorescence spectroscopy (ed.) J R Lakowicz (New York: Plenum) vol. 2, pp 1-52
16. Swaminathan R, Nath U, Udgaonkar J B, Periasamy N and Krishnamoorthy G 1996 Biochemistry 35 9150
17. Swaminathan R, Periasamy N, Udgaonkar J B and Krishnamoorthy G 1994 J. Phys. Chem. 98 9270
18. Swaminathan R, Krishnamoorthy G and Periasamy N 1994 Biophys. J. 672013
19. Lakowicz J R 1996 Principles of fluorescence spectroscopy (New York: Plenum) pp. 297-298
20. Maity H and Jarori G K 2002 Physiol. Chem. Phys. & Med. NMR 34 43
21. Swaminathan R 1995 Time-resolved fluorescence studies in biological macromolecules, Ph D thesis, University of Bombay, Mumbai
22. Matsuura H, Shimotakahara S, Sakuma C, Tashiro M, Shindo H, Mochizuki K, Yamagishi A, Kojima M and Takahashi K 2004 Biol. Chem. 385 1157
23. Bhavesh N S, Juneja J, Udgaonkar J B and Hosur R V 2004 Protein Sci. 13 3085
24. Wong K B, Freund S M V and Fersht A R 1996 J. Mol. Biol. 259 805
25. Saxena A M, Udgaonkar J B and Krishnamoorthy G 2006 J. Mol. Biol. 359 174