A continuous-exchange cell-free protein synthesis system fabricated on a chip

Analytical Biochemistry

Volumn 365, Issue 2, 2007, Pages 280-282

  Hahn, Geun Hee ^a   Asthana, Amit ^a   Kim, Dong Myung ^a   Kim, Dong Pyo ^a  

^a Chungnam National University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

CELL FREE PROTEIN SYNTHESIS;

BIOSYNTHESIS;

CHLORAMPHENICOL ACETYLTRANSFERASE; DIMETICONE; GREEN FLUORESCENT PROTEIN;

ARTICLE; CELL FREE SYSTEM; DIALYSIS MEMBRANE; MICROREACTOR; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; REACTOR DESIGN;

EID: 34247887375     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.03.025     Document Type: Article

References (12)

1. Sawasaki T., Ogasawara T., Morishita R., and Endo Y. A cell-free protein synthesis system for high-throughput proteomics. Proc. Natl. Acad. Sci. USA 99 (2002) 14652-14657
2. Kang T.J., Song H.K., Ahn J.H., Choi C.Y., and Joo H. Optimization of programmed suppression in a cell-free protein synthesis system with unnatural amino acid S-(2-nitrobenzyl)cysteine. J. Microbiol. Biotechnol. 13 (2003) 344-347
3. Ahn J.H., Son J.M., Hwang M.Y., Kim T.W., Park C.K., Choi C.Y., and Kim D.M. Enhanced in vitro protein synthesis through optimal design of PCR primers. J. Microbiol. Biotechnol. 16 (2006) 355-359
4. Kim D.M., and Swartz J.R. Prolonging cell-free protein synthesis with a novel ATP regeneration system. Biotechnol. Bioeng. 66 (1999) 180-188
5. Michel-Reydellet N., Calhoun K., and Swartz J. Amino acid stabilization for cell-free protein synthesis by modification of the Escherichia coli genome. Metab. Eng. 6 (2004) 197-203
6. Kim D.M., and Choi C.Y. A semicontinuous prokaryotic coupled transcription/translation system using a dialysis membrane. Biotechnol. Prog. 12 (1996) 645-649
7. Spirin A.S., Baranov V.I., Ryabova L.A., Ovodov S.Y., and Alakhov Y.B. A continuous cell-free translation system capable of producing polypeptides in high yield. Science 242 (1988) 1162-1164
8. Katzen F., Chang G., and Kudlicki W. The past, present, and future of cell-free protein synthesis. Trends Biotechnol. 23 (2005) 150-156
9. Kinpara T., Mizuno R., Murakami Y., Kobayashi M., Yamaura S., Hasan Q., Morita Y., Nakano H., Yamane T., and Tamiya E. A picoliter chamber array for cell-free protein synthesis. J. Biochem. 136 (2004) 149-154
10. McDonald J.C., Duffy D.C., Anderson J.R., Chiu D.T., Wu H., Schueller O.J.A., and Whitesides G.M. Fabrication of microfluidic systems in poly(dimethylsiloxane). Electrophoresis 21 (2000) 27-40
11. Schagger H., and von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166 (1987) 368-379
12. Shaw W.V. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria. Methods Enzymol. 43 (1975) 737-755