Laboratory diagnosis of antibiotic-associated diarrhea: a Polish pilot study into the clinical relevance of Clostridium difficile and Clostridium perfringens toxins

Diagnostic Microbiology and Infectious Disease

Volumn 58, Issue 1, 2007, Pages 71-75

  Pituch, Hanna ^a   Obuch Woszczatyński, Piotr ^a   Wultańska, Dorota ^a   van Belkum, Alex ^b   Meisel Mikołajczyk, Felicja ^a   Łuczak, Mirosław ^a  

^a MEDICAL UNIVERSITY OF WARSAW   (Poland)

^b ERASMUS MEDICAL CENTER   (Netherlands)

Author keywords

AAD; Antibiotic associated diarrhea; Clostridium difficile; Clostridium perfringens; Metronidazole

Indexed keywords

ENTEROTOXIN; METRONIDAZOLE; TCDA TOXIN; TCDB TOXIN; TOXIN; UNCLASSIFIED DRUG;

ANTIBIOTIC ASSOCIATED DIARRHEA; ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM ISOLATE; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM PERFRINGENS; DIARRHEA; FECES; GENE; HOSPITAL PATIENT; HUMAN; IN VITRO STUDY; LABORATORY DIAGNOSIS; MAJOR CLINICAL STUDY; PILOT STUDY; PREVALENCE; PRIORITY JOURNAL; SAMPLING; SCREENING;

ANTI-INFECTIVE AGENTS; BACTERIAL PROTEINS; BACTERIAL TOXINS; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM PERFRINGENS; DIARRHEA; ENTEROCOLITIS, PSEUDOMEMBRANOUS; ENTEROTOXINS; FECES; HUMANS; METRONIDAZOLE; MICROBIAL SENSITIVITY TESTS; PILOT PROJECTS; POLAND; POLYMERASE CHAIN REACTION; PREVALENCE;

EID: 34247880453     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2006.12.007     Document Type: Article

References (28)

1. Abrahao C., Carman R.J., Hahn H., and Liesenfeld O. Similar frequency of detection of Clostridium perfringens enterotoxin and Clostridium difficile toxins in patients with antibiotic-associated diarrhea. Eur. J. Clin. Microbiol. Infect. Dis. 20 (2001) 676-677
2. Ackermann G., Thomalla S., Ackerman F., Schaumann R., Rodloff AC., and Ruf B.R. Prevalence and characteristics of bacteria and host factors in an outbreak situation of antibiotic-associated diarrhoea. J. Med. Microbiol. 54 (2005) 149-153
3. Asha N.J., and Wilcox M.H. Laboratory diagnosis of Clostridium perfringens antibiotic-associated diarrhoea. J. Med. Microbiol. 51 (2002) 891-894
4. Barbut F., Corthier G., Charpak Y., et al. Prevalence and pathogenicity of Clostridium difficile in hospitalized patients. A French multi-center study. Arch. Intern. Med. 156 (1996) 1449-1454
5. Berry P.R., Rodhouse J.C., Hughes S., Bartholomew B.A., and Gilbert R.J. Laboratory techniques. Evaluation of ELISA, RPLA and Vero cell assays for detecting of Clostridium perfringens enterotoxin in faecal specimens. J. Clin. Pathol. 41 (1988) 458-461
6. Boone J.H., and Carman R.J. Clostridium perfringens: food poisoning and antibiotic associated diarrhea. Clin. Microbiol. Newsl. 19 (1997) 65-67
7. Borriello S.P. Pathogenesis of Clostridium difficile infection. J Antimicrob Chemother 41 Suppl C (1998) 13-19
8. Borriello S.P., and Williams R.KT. Treatment of Clostridium perfringens enterotoxin-associated diarrhoea with metronidazole. J. Infect. 10 (1985) 65-67
9. Borriello S.P., Larson H.E., Welch A.R., Barclay F., Stringer M.F., and Bartholomew B.A. Enterotoxigenic Clostridium perfringens: a possible cause of antibiotic-associated diarrhoea. Lancet 1 (1984) 305-307
10. Carman R.J. Clostridium perfringens in spontaneous and antibiotic-associated diarrhoea of man and other animals. Rev. Med. Microbiol. 8 Suppl (1997) 43-45
11. Daube G., Simon P., Limbourg C., Manteca C., Mainil J., and Kaeckenbeeck A. Hybridization of 2,659 Clostridium perfringens isolates with gene probes for seven toxins (α, β, ε, ι, θ, μ and enterotoxin) and for sialidase. Am. J. Vet. Res. 57 (1996) 496-501
12. Ellner P.D. A medium promoting rapid quantitative sporulation in C. perfringens. J. Bacteriol. 71 (1956) 495-496
13. Fach P., and Popoff M.R. Detection of enterotoxigenic Clostridium perfringens in food and fecal samples with a duplex PCR and the slide latex agglutination test. Appl. Environ. Microbiol. 63 (1997) 4232-4236
14. Gal M., and Brazier J.S. Metronidazole resistance in Bacteroides spp. carrying nim genes and the selection of slow-growing metronidazole-resistant mutants. J. Antimicrob. Chemother. 54 (2004) 109-116
15. Hancock P. Antibiotic-associated diarrhoea: Clostridium difficile or Clostridium perfringens?. Rev. Med. Microbiol. 8 Suppl 1 (1997) S66-S67
16. Hatheway C.L. Toxigenic Clostridia. Clin. Microbiol. Rev. 3 (1990) 66-98
17. Heimesaat M.M., Granzow K., Leidinger H., and Liesenfeld O. Prevalence of Clostridium difficile toxins A and B and Clostridium perfringens enterotoxin A in stool samples of patients with antibiotic-associated diarrhea. Infection 33 (2005) 340-344
18. Joshy L., Dhawan B., and Chaudhry R. Antibiotic associated diarrhoea-possible role of Clostridium species. Abstract book of XXVII National Conference of Indian Association of Medical Microbiologists (2003) 148 Mumbai
19. Modi N., and Wilcox M.H. Evidence for antibiotic induced Clostridium perfringens diarrhoea. J. Clin. Pathol. 54 (2001) 748-751
20. Perelle S., Gibert M., Bourlioux P., Corthier G., and Popoff M.R. Production of a complete binary toxin (actin-specific ADP-ribosyltransferase) by Clostridium difficile CD196. Infect. Immun. 65 (1997) 1402-1407
21. Pituch H., van den Braak N., van Belkum A., et al. Characterization of Clostridium perfringens strains isolated from Polish patients with suspected antibiotic-associated diarrhea. Med. Sci. Monit. 8 (2002) BR85-BR88
22. Pituch H., van Belkum A., van den Braak N., Obuch-Woszczatyñski P., Verbrugh H., Meisel-Mikołajczyk F., and Łuczak M. Recent emergence of an epidemic clindamycin-resistant clone of Clostridium difficile among Polish patients with C. difficile-associated diarrhea. J. Clin. Microbiol. 41 (2003) 4184-4187
23. Pituch H., Rupnik M., Obuch-Woszczatyñski P., Grubesic A., Meisel-Mikołajczyk F., and Łuczak M. Detection of binary-toxin genes (cdtA and cdtB) among Clostridium difficile strains isolated from patients with C. difficile-associated diarrhoea (CDAD) in Poland. J. Med. Microbiol. 54 (2005) 143-147
24. B) type resistance. J. Med. Microbiol. 55 (2006) 207-213
25. Stubbs S.L., Rupnik M., Gibert M., Brazier J.S., Duerden B.I., and Popoff MR. Production of actin-specific ADP-ribosyltransferase (binary toxin) by strains of Clostridium difficile. FEMS Microbiol. Lett. 186 (2000) 307-312
26. Vaishnavi C.H., Kaur S., and Singh K. Clostridium perfringens type A & antibiotic associated diarrhoea. Indian. J. Med. Res. 122 (2005) 52-56
27. Van Damme-Jongsten M., Wernars K., and Notermans S. Cloning and sequencing of the Clostridium perfringens enterotoxin gene. Antonie van Leeuwenhoek 56 (1989) 181-190
28. Wilcox M.H., and Smyth E.T. Incidence and impact of C. difficile infection in the UK, 1993-1996. J. Hosp. Infect. 34 (1998) 23-30