Acid-base catalysis in Leuconostoc mesenteroides sucrose phosphorylase probed by site-directed mutagenesis and detailed kinetic comparison of wild-type and Glu237 → Gln mutant enzymes

Biochemical Journal

Volumn 403, Issue 3, 2007, Pages 441-449

  Schwarz, Alexandra ^a   Brecker, Lothar ^b   Nidetzky, Bernd ^a  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b UNIVERSITY OF VIENNA   (Austria)

Author keywords

Br nsted catalysis; Chemical rescue; Family GH 13; Phosphorylase; pKa modulation; Retaining mechanism

Indexed keywords

ACID BASE CATALYSIS; ANIONIC NUCLEOPHILES; DEGLUCOSYLATION; GLUTAMATE; LEUCONOSTOC MESENTEROIDES; SUCROSE PHOSPHORYLASE;

ACIDITY; ALKALINITY; AMINO ACIDS; MUTAGENESIS; NUCLEAR MAGNETIC RESONANCE; NUCLEOPHILES; SUGAR (SUCROSE);

ENZYME KINETICS;

ACETIC ACID; AZIDE; BACTERIAL ENZYME; FORMIC ACID; HALIDE; PHOSPHORYLASE; SUCROSE PHOSPHORYLASE; UNCLASSIFIED DRUG; GLUCOSYLTRANSFERASE; GLUTAMIC ACID;

ACID BASE BALANCE; ARTICLE; CATALYSIS; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME ASSAY; GLYCOSYLATION; KINETICS; LEUCONOSTOC MESENTEROIDES; NUCLEAR MAGNETIC RESONANCE; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; STEADY STATE; AMINO ACID SEQUENCE; CHEMISTRY; ENZYMOLOGY; GENETICS; LEUCONOSTOC; METABOLISM; POINT MUTATION;

LEUCONOSTOC MESENTEROIDES;

AMINO ACID SEQUENCE; CATALYSIS; GLUCOSYLTRANSFERASES; GLUTAMIC ACID; HYDROGEN-ION CONCENTRATION; KINETICS; LEUCONOSTOC; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION;

EID: 34247867966     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20070042     Document Type: Article

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