A modified method for the detection of microbial proteases on agar plates using tannic acid

Journal of Biochemical and Biophysical Methods

Volumn 70, Issue 4, 2007, Pages 697-699

  Saran, Saurabh ^a   Isar, Jasmine ^a   Saxena, Rajendra Kumar ^a  

^a Department of Microbiology ^*  (India)

Author keywords

Detection; Microorganism; Protease; Tannic acid

Indexed keywords

AGAR; MICROBIAL ENZYME; PROTEINASE; TANNIN; TRICHLOROACETIC ACID;

ARTICLE; ASPERGILLUS NIGER; BACILLUS LICHENIFORMIS; BACTERIUM ISOLATION; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ASSAY; FUNGUS ISOLATION; NONHUMAN; PRIORITY JOURNAL;

ASPERGILLUS NIGER; BACILLUS; BACTERIAL PROTEINS; ELECTROPHORESIS, AGAR GEL; FUNGAL PROTEINS; PEPTIDE HYDROLASES; SOIL MICROBIOLOGY; TANNINS;

BACTERIA (MICROORGANISMS); FUNGI;

EID: 34247642734     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbbm.2007.03.005     Document Type: Article

References (11)

1. Kumar C.G., and Takagi H. Microbial alkaline protease: from a bioindustrial view point. Biotechnol Adv 17 (1998) 561-594
2. Joo H.S., Kumar C.G., Park G.C., Kim K.M., Paik S.R., and Chang C.S. Optimization of the production of an extracellular alkaline protease from Bacillus horikoshii. Process Biochem 38 (2002) 155-159
3. Prakasham R.S., Rao C.S., Rao R.S., and Sharma P.N. Alkaline protease production by an isolated Bacillus circulans under solid-state fermentation using agro-industrial waste: process parameters optimization. Biotechnol Prog 21 (2005) 1380-1388
4. Thys R.C.S., Guzzon S.O., Cladera-Olivera F., and Brandelli A. Optimization of protease production by Microbacterium sp. in feather meal using response surface methodology. Process Biochem 41 (2006) 67-73
5. Laxman R.S., Sonawane A.P., More S.V., Rao B.S., Rele M.V., Jogdand V.V., et al. Optimization and scale up of production of alkaline protease from Conidiobolus coronatus. Process Biochem 40 (2005) 3152-3158
6. Banerjee U.C., Sani R.K., Azmi W., and Soni R. Thermostable alkaline protease from Bacillus brevis and its characterization as a laundry determine additive. Process Biochem 35 (1999) 213-219
7. Tari C., Genckal H., and Tokatli F. Optimization of a growth medium using a statistical approach for the production of an alkaline protease from a newly isolated Bacillus sp. L 21. Process Biochem 41 (2006) 659-665
8. Godfrey T., and West S. Introduction to Industrial Enzymology (1996), Macmillan Press, UK 1-8
9. Lantz M.S., and Ciborowski P. Zymographic techniques for detection and characterization of microbial proteases. Methods Enzymol 235 (1994) 563-594
10. Hagerman A.E., and Butler L.G. Protein precipitation method for the quantification determination of tannins. J Agric Food Chem 24 (1978) 809-812
11. Carbonaro M., Grant G., and Pusztai A. Evaluation of polyphenol bioavailabilty in rat small intestine. Eur J Nutr 40 (2001) 84-90