RNAi depleted Drosophila cell extracts to dissect signaling pathways leading to actin polymerization

Journal of Biochemical and Biophysical Methods

Volumn 70, Issue 4, 2007, Pages 663-669

  Bouslama Oueghlani, Lamia ^a   Echard, Arnaud ^a   Louvard, Daniel ^a   Gautreau, Alexis ^a  

^a INSTITUT CURIE   (France)

Author keywords

Actin polymerization; Arp2 3 complex; Coated beads; Rho GTPase; Wasp

Indexed keywords

ACTIN; ACTIN RELATED PROTEIN 2; ACTIN RELATED PROTEIN 3; CELL EXTRACT; DOUBLE STRANDED RNA;

ACTIN POLYMERIZATION; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL CULTURE; CONTROLLED STUDY; DROSOPHILA; GENOME; NONHUMAN; PRIORITY JOURNAL; RNA INTERFERENCE; SIGNAL TRANSDUCTION;

ACTINS; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; DNA, COMPLEMENTARY; DROSOPHILA; DROSOPHILA PROTEINS; OPEN READING FRAMES; RECOMBINANT PROTEINS; RNA INTERFERENCE; SEQUENCE DELETION; SIGNAL TRANSDUCTION;

VERTEBRATA;

EID: 34247593750     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbbm.2007.03.003     Document Type: Article

References (29)

1. Pollard T.D., and Borisy G.G. Cellular motility driven by assembly and disassembly of actin filaments. Cell 112 (2003) 453-465
2. Burridge K., and Wennerberg K. Rho and Rac take center stage. Cell 116 (2004) 167-179
3. Welch M.D., Iwamatsu A., and Mitchison T.J. Actin polymerization is induced by Arp2/3 protein complex at the surface of Listeria monocytogenes. Nature 385 (1997) 265-269
4. Ma L., Cantley L.C., Janmey P.A., and Kirschner M.W. Corequirement of specific phosphoinositides and small GTP-binding protein Cdc42 in inducing actin assembly in Xenopus egg extracts. J Cell Biol 140 (1998) 1125-1136
5. Ma L., Rohatgi R., and Kirschner M.W. The Arp2/3 complex mediates actin polymerization induced by the small GTP-binding protein Cdc42. Proc Natl Acad Sci U S A 95 (1998) 15362-15367
6. Rohatgi R., Ma L., Miki H., Lopez M., Kirchhausen T., Takenawa T., et al. The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly. Cell 97 (1999) 221-231
7. Ho H.Y., Rohatgi R., Lebensohn A.M., Le Ma, Li J., Gygi S.P., et al. Toca-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Cell 118 (2004) 203-216
8. Clemens J.C., Worby C.A., Simonson-Leff N., Muda M., Maehama T., Hemmings B.A., et al. Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways. Proc Natl Acad Sci U S A 97 (2000) 6499-6503
9. Saleh M.C., van Rij R.P., Hekele A., Gillis A., Foley E., O'Farrell P.H., et al. The endocytic pathway mediates cell entry of dsRNA to induce RNAi silencing. Nat Cell Biol 8 (2006) 793-802
10. Gautreau A., Ho H.Y., Li J., Steen H., Gygi S.P., and Kirschner M.W. Purification and architecture of the ubiquitous Wave complex. Proc Natl Acad Sci U S A 101 (2004) 4379-4383
11. Self A.J., and Hall A. Purification of recombinant Rho/Rac/G25K from Escherichia coli. Methods Enzymol 256 (1995) 3-10
12. Foley E., and O'Farrell P.H. Functional dissection of an innate immune response by a genome-wide RNAi screen. PLoS Biol 2 (2004) E203
13. He B., Rong M., Lyakhov D., Gartenstein H., Diaz G., Castagna R., et al. Rapid mutagenesis and purification of phage RNA polymerases. Protein Expr Purif 9 (1997) 142-151
14. Machesky L.M., Mullins R.D., Higgs H.N., Kaiser D.A., Blanchoin L., May R.C., et al. Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complex. Proc Natl Acad Sci U S A 96 (1999) 3739-3744
15. Bernheim-Groswasser A., Wiesner S., Golsteyn R.M., Carlier M.F., and Sykes C. The dynamics of actin-based motility depend on surface parameters. Nature 417 (2002) 308-311
16. van der Gucht J., Paluch E., Plastino J., and Sykes C. Stress release drives symmetry breaking for actin-based movement. Proc Natl Acad Sci U S A 102 (2005) 7847-7852
17. Robinson R.C., Turbedsky K., Kaiser D.A., Marchand J.B., Higgs H.N., Choe S., et al. Crystal structure of Arp2/3 complex. Science 294 (2001) 1679-1684
18. Gournier H., Goley E.D., Niederstrasser H., Trinh T., and Welch M.D. Reconstitution of human Arp2/3 complex reveals critical roles of individual subunits in complex structure and activity. Mol Cell 8 (2001) 1041-1052
19. Kunda P., Craig G., Dominguez V., and Baum B. Abi, Sra1, and Kette control the stability and localization of SCAR/WAVE to regulate the formation of actin-based protrusions. Curr Biol 13 (2003) 1867-1875
20. Rogers S.L., Wiedemann U., Stuurman N., and Vale R.D. Molecular requirements for actin-based lamella formation in Drosophila S2 cells. J Cell Biol 162 (2003) 1079-1088
21. Aspenstrom P., Fransson A., and Saras J. Rho GTPases have diverse effects on the organization of the actin filament system. Biochem J 377 (2004) 327-337
22. Rivero F., Dislich H., Glockner G., and Noegel A.A. The Dictyostelium discoideum family of Rho-related proteins. Nucleic Acids Res 29 (2001) 1068-1079
23. Wiesner S., Helfer E., Didry D., Ducouret G., Lafuma F., Carlier M.F., et al. A biomimetic motility assay provides insight into the mechanism of actin-based motility. J Cell Biol 160 (2003) 387-398
24. Lebensohn A.M., Ma L., Ho H.Y., and Kirschner M.W. Cdc42 and PI(4,5)P2-induced actin assembly in Xenopus egg extracts. Methods Enzymol 406 (2006) 156-173
25. Echeverri C.J., and Perrimon N. High-throughput RNAi screening in cultured cells: a user's guide. Nat Rev Genet 7 (2006) 373-384
26. Arziman Z., Horn T., and Boutros M. E-RNAi: a web application to design optimized RNAi constructs. Nucleic Acids Res 33 (2005) W582-W588
27. Moreau V., and Way M. Cdc42 is required for membrane dependent actin polymerization in vitro. FEBS Lett 427 (1998) 353-356
28. Zigmond S.H., Joyce M., Borleis J., Bokoch G.M., and Devreotes P.N. Regulation of actin polymerization in cell-free systems by GTPgamma S and Cdc42. J Cell Biol 138 (1997) 363-374
29. Oikawa T., Yamaguchi H., Itoh T., Kato M., Ijuin T., Yamazaki D., et al. PtdIns(3,4,5)P3 binding is necessary for WAVE2-induced formation of lamellipodia. Nat Cell Biol 6 (2004) 420-426