The key enzyme in galactose metabolism, UDP-galactose-4-epimerase, affects cell-wall integrity and morphology in Candida albicans even in the absence of galactose

Fungal Genetics and Biology

Volumn 44, Issue 6, 2007, Pages 563-574

  Singh, Vijender ^a   Satheesh, Somisetty V ^a   Raghavendra, Mysore L ^a   Sadhale, Parag P ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

Biofilm; Candida albicans; Cell wall; GAL10; Oxidative stress; Virulence

Indexed keywords

ALDOSE 1 EPIMERASE; ALPHA GALACTOSIDASE; BETA GALACTOSIDASE; EPIMERASE; GAL10 PROTEIN; GALACTOSE; GENE PRODUCT; HYDROGEN PEROXIDE; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE GLUCOSE 4 EPIMERASE;

ARTICLE; BIOFILM; CANDIDA ALBICANS; CELL MUTANT; CELL STRUCTURE; CELL SURVIVAL; CELL WALL; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME METABOLISM; EPIMERIZATION; FUNGAL COLONIZATION; FUNGAL STRAIN; FUNGUS CULTURE; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATIVE STRESS; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; WILD TYPE;

AMINO ACID SEQUENCE; CANDIDA ALBICANS; CELL WALL; GALACTOSE; GENES, FUNGAL; MOLECULAR SEQUENCE DATA; MUTATION; UDPGLUCOSE 4-EPIMERASE;

CANDIDA ALBICANS; FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 34247585795     PISSN: 10871845     EISSN: 10960937     Source Type: Journal    
DOI: 10.1016/j.fgb.2006.11.006     Document Type: Article

References (32)

1. Ausubel F.M., Brent R., Kingston R., Moore D., Seidman J., Smith J.A., and Struhl K. Current protocols in Molecular Biology (1987-1994), John Wiley and Sons, Chichester
2. Baillie G.S., and Douglas L.J. Matrix polymers of Candida biofilms and their possible role in biofilm resistance to antifungal agents. J. Antimicrob. Chemother. 46 3 (2000) 397-403
3. Barber C., Rosti J., Rawat A., Findlay K., Roberts K., and Seifert G.J. Distinct properties of the five UDP-d-glucose/UDP-d-galactose 4-epimerase isoforms of Arabidopsis thaliana. J. Biol. Chem. 281 25 (2006) 17276-17285
4. Bhat P.J., and Murthy T.V. Transcriptional control of the GAL/MEL regulon of yeast Saccharomyces cerevisiae: mechanism of galactose mediated signal transduction. Mol. Microbiol. 40 5 (2001) 1059-1066
5. Cardinali G., Vollenbroich V., Jeon M.-S., De Graaf A.A., and Hollenberg C.P. Constitutive expression in gal7 mutants of Kluyveromyces lactis is due to internal production of galactose as an inducer of the gal/lac regulon. Mol. Cell. Biol. 17 3 (1997) 1722-1730
6. Chappel T.G., and Warren G. A galactosyl transferase from the fission yeast Schizosaccharomyces pombe. J. Cell Biol. 109 (1989) 2693-2702
7. Copping V.M., Barelle C.J., Hube B., Gow N.A., Brown A.J., and Odds F.C. Exposure of Candida albicans to antifungal agents affects expression of SAP2 and SAP9 secreted proteinase genes. J. Antimicrob. Chemother. 55 5 (2005) 645-654
8. Ernst J.F. Transcription factors in Candida albicans-environmental control of morphogenesis. Microbiology 146 (2000) 1763-1774
9. Frey P.A. The Leloir pathway: a mechanistic imperative for three enzymes to change the stereochemical configuration of a single carbon in galactose. FASEB J. 10 4 (1996) 461-470
10. Gillum A.M., Tsay E.Y., and Kirsch D.R. Isolation of the Candida albicans gene for orotidine-5_-phosphate decarboxylase by complementation of S. cerevisiae ura3 and E. coli pyrF mutations. Mol. Gen. Genet. 198 (1984) 179-182
11. Gola S., Martin R., Walther A., Dünkler A., and Wendland J. New modules for PCR-based gene targeting in Candida albicans: rapid and efficient gene targeting using 100 bp of flanking homology region. Yeast 20 (2003) 1339-1347
12. Gow N., Brown A., and Odds F. Fungal morphogenesis and host invasion. Curr. Opin. Microbiol. 5 (2002) 366
13. Holden H.M., Rayment I., and Thoden J.B. Structure and function of enzymes of the Leloir pathway for galactose metabolism. J. Biol. Chem. 278 45 (2003) 43885-43888
14. Huang H.M., and Snider M.D. Isolation of protein glycosylation mutants in the fission yeast Schizosaccharomyces pombe. Mol. Biol. Cell. 6 (1995) 485-496
15. Jabra-Rizk M.A., Falkler W.A., and Meiller T.F. Fungal biofilms and drug resistance. Emerg. Infect. Dis. 10 1 (2004) 14-19
16. Jin Y., Samaranayake L.P., Samaranayake Y., and Yip H.K. Biofilm formation of Candida albicans is variably affected by saliva and dietary sugars. Arch. Oral Biol. 49 10 (2004) 789-798
17. Lo H.J., Kohler J.R., DiDomenico B., Loebenberg D., Cacciapuoti A., and Fink G.R. Nonfilamentous C. albicans mutants are avirulent. Cell. 90 (1997) 939-949
18. McCourtie J., and Douglas L.J. Relationship between cell surface composition, adherence, and virulence of Candida albicans. Infect. Immun. 45 1 (1984) 6-12
19. Mehta D.V., Kabir A., and Bhat P.J. Expression of human inositol monophosphatase suppresses galactose toxicity in Saccharomyces cerevisiae: possible implications in galactosemia. Biochim. Biophys. Acta. 1454 (1999) 217-226
20. Nikawa H., Egusa H., Makihira S., Yamashiro H., Fukushima H., Jin C., Nishimura M., Pudji R.R., and Hamada T. Alteration of the coadherence of Candida albicans with oral bacteria by dietary sugars. Oral Microbiol. Immunol. 16 5 (2001) 279-283
21. Quimby B.B., Alano A., Almashanu S., DeSandro A.M., Cowan T.M., and Fridovich-Keil J.L. Characterization of two mutations associated with epimerase-deficiency galactosemia, by use of a yeast expression system for human UDP-galactose-4-epimerase. Am. J. Hum. Genet. 61 (1997) 590-598
22. Richardson M.D., and Smith H. Ultrastructural features of phagocytosis and intracellular killing of Candida albicans by mouse polymorphonuclear phagocyte monolayers. Mycopathologia 83 (1983) 97-102
23. Roper J.R., Güther M.L., Milne K.G., and Ferguson M.A.J. Galactose metabolism is essential for the African sleeping sickness parasite Trypanosoma brucei. Proc. Natl. Acad. Sci. USA 99 (2002) 5884-5889
24. Samaranayake L.P., and MacFarlane T.W. The effect of dietary carbohydrates on the in vitro adhesion of Candida albicans to epithelial cells. J. Med. Microbiol. 15 (1982) 511-517
25. Samaranayake Y.H., Ye J., Yau J.Y., Cheung B.P., and Samaranayake L.P. In vitro method to study antifungal perfusion in Candida biofilms. J. Clin. Microbiol. 43 2 (2005) 818-825
26. Seiboth B., Karaffa L., Sandor E., and Kubicek C. The Hypocrea jecorina gal10 (uridine 50-diphosphate-glucose 4-epimerase-encoding) gene differs from yeast homologs in structure, genomic organization and expression. Gene 295 (2002) 143-149
27. Sherman F., Fink G.R., and Hicks J. Laboratory Course Manual for Methods in Yeast Genetics (1986), Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
28. Singh V., Sinha I., and Sadhale P.P. Global analysis of altered gene expression during morphogenesis of Candida albicans in vitro. Biochem. Biophys. Res. Commun. 334 (2005) 1149-1158
29. Thoden J.B., and Holden H.M. The molecular architecture of galactose mutarotase/UDP-galactose 4-epimerase from Saccharomyces cerevisiae. J. Biol. Chem. 280 (2005) 21900-21907
30. Vijayraghavan U., Company M., and Abelson J. Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev. 3 (1989) 1206-1216
31. Walther A., and Wendland J. An improved transformation protocol for the human fungal pathogen Candida albicans. Curr. Genet. 42 (2003) 339-343
32. Wilson R.B., Davis D., and Mitchell A.P. Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions. J. Bacteriol. 181 (1999) 1868-1874