Quantification of fibronectin matrix assembly sites using a novel ELISA assay

Matrix Biology

Volumn 26, Issue 4, 2007, Pages 330-333

  Zheng, Mingzhe ^a   Ambesi, Anthony ^a   Yu, Lin ^a   McKeown Longo, Paula J ^a  

^a ALBANY MEDICAL COLLEGE   (United States)

Author keywords

Cell layer ELISA; Extracellular matrix; Fibronectin; Fibronectin matrix assembly sites; Vitronectin

Indexed keywords

FIBRONECTIN; IODINE 125;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL CULTURE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EXTRACELLULAR MATRIX; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN POLYMERIZATION; PROTEIN PURIFICATION; QUANTITATIVE ANALYSIS;

ANIMALS; CELL LINE; CELLS, CULTURED; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME-LINKED IMMUNOSORBENT ASSAY; EXTRACELLULAR MATRIX; FIBRONECTINS; HUMANS; MICE; MICROSCOPY, FLUORESCENCE; PROTEIN STRUCTURE, TERTIARY; VITRONECTIN;

EID: 34247541329     PISSN: 0945053X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.matbio.2006.12.004     Document Type: Article

References (12)

1. Bae E., Sakai T., and Mosher D.F. Assembly of exogenous fibronectin by fibronectin-null cells is dependent on the adhesive substrate. J. Biol. Chem. 279 (2004) 35749-35759
2. Christopher R.A., Kowalczyk A.P., and McKeown-Longo P.J. Localization of fibronectin matrix assembly sites on fibroblasts and endothelial cells. J. Cell Sci. 110 (1997) 569-581
3. Hocking D.C., Sottile J., and McKeown-Longo P.J. Fibronectin's III-1 module contains a conformation-dependent binding site for the amino-terminal region of fibronectin. J. Biol. Chem. 269 (1994) 19183-19191
4. Hocking D.C., Smith R.K., and McKeown-Longo P.J. A novel role for the integrin-binding III-10 module in fibronectin matrix assembly. J. Cell Biol. 133 (1996) 431-444
5. Hocking D.C., Sottile J., Reho T., Fassler R., and McKeown-Longo P.J. Inhibition of fibronectin matrix assembly by the heparin-binding domain of vitronectin. J. Biol. Chem. 274 (1999) 27257-27264
6. McKeown-Longo P.J., and Mosher D.F. Interaction of the 70 kilodalton amino-terminal fragment of fibronectin with the matrix-assembly receptor of fibroblasts. J. Cell Biol. 100 (1985) 364-374
7. McLeskey S.W., Kurebayashi J., Honig S.F., Zwiebel J., Lippman M.E.D.R.B., and Kern F.G. Fibroblast growth factor 4 transfection of MCF-7 cells produces cell lines that are tumorigenic and metastatic in ovariectomized or tamoxifen-treated athymic nude mice. Cancer Res. 53 (1993) 2168-2177
8. Pankov R., Cukierman E., Katz B.Z., Matsumoto K., Lin D.C., Lin S., Hahn C., and Yamada K.M. Integrin dynamics and matrix assembly: tensin-dependent translocation of α5β1 integrins promotes early fibronectin fibrillogenesis. J. Cell Biol. 148 (2000) 1075-1090
9. Saoncella S., Echtermeyer F., Dehnez F., Nowlen J.K., Mosher D.F., Robinson S.D., Hynes R.O., and Goetinck P.F. Syndecan-4 signals cooperatively with integrins in a Rho-dependent manner in the assembly of focal adhesions and actin stress fibers. Proc. Natl. Acad. Sci. U. S. A. 96 (1999) 2805-2810
10. 2-terminal region of fibronectin to molecules of large apparent molecular mass. J. Biol. Chem. 271 (1996) 33284-33292
11. Zhang Q., Sakai T., Nowlen J., Hayashi I., Fassler R., and Mosher D.F. Functional β1-integrins release the suppression of fibronectin matrix assembly by vitronectin. J. Biol. Chem. 274 (1999) 368-375
12. Zheng M., and McKeown-Longo P.J. Regulation of HEF1 expression and phosphorylation by TGF-β1 and cell adhesion. J. Biol. Chem. 277 (2002) 39599-39608