Cloning and characterization of a novel Δ12-fatty acid desaturase gene from the tree Sapium sebiferum

Biotechnology Letters

Volumn 29, Issue 6, 2007, Pages 959-964

  Niu, Bei ^a   Ye, Huaxun ^a   Xu, Ying ^a   Wang, Shenghua ^a   Chen, Peng ^a   Peng, Shuming ^a   Ou, Yangchao ^a   Tang, Lin ^a   Chen, Fang ^a  

^a SICHUAN UNIVERSITY   (China)

Author keywords

12 fatty acid desaturase; Cloning; Saccharomyces cerevisiae; Sapium sebiferum

Indexed keywords

CLONING; FATTY ACIDS; FORESTRY; YEAST;

AMINO ACID SEQUENCES; GENOMIC SOUTHERN BLOT ANALYSIS; PROTEINS ENCODING; SAPIUM SEBIFERUM;

GENES;

ACYL COENZYME A DESATURASE; FATTY ACID; RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ENZYMOLOGY; GENE EXPRESSION PROFILING; GENETICS; GENOME; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLANT LEAF; PLANT ROOT; PLANT SEED; PLANT STEM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SAPIUM; SEQUENCE HOMOLOGY; SOUTHERN BLOTTING;

AMINO ACID SEQUENCE; BLOTTING, SOUTHERN; CLONING, MOLECULAR; FATTY ACID DESATURASES; FATTY ACIDS; GENE EXPRESSION PROFILING; GENOME, PLANT; MOLECULAR SEQUENCE DATA; PLANT LEAVES; PLANT PROTEINS; PLANT ROOTS; PLANT STEMS; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SAPIUM; SEEDS; SEQUENCE HOMOLOGY, AMINO ACID;

CLONES; FATTY ACIDS; FORESTS; GENES; YEASTS;

SACCHAROMYCES CEREVISIAE; SAPIUM; TRIADICA SEBIFERA;

EID: 34247398682     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-007-9325-6     Document Type: Article

References (15)

1. Behrouzian B, Buist PH (2003) Bioorganic chemistry of plant lipid desaturation. Phytochem Rev 2:103-111
2. Chen Y, Yang GZ, Zhang SL, Zhu ZF (1999) Review of the chemical constituents isolated from Sapium sebiferum (L.) roxb. Nat Prod Res Dev 11:114-120
3. Heppard EP, Kinney AJ, Stecca KL, Miao GH (1996) Developmental and growth temperature regulation of two different microsomal ω-6 desaturase genes in soybeans. Plant Physiol 110:311-319
4. -ΔΔCT method. Methods 25:402-408
5. Martínez-Rivas JM, Sperling P, Lühs W, Heinz E (2001) Spatial and temporal regulation of three different microsomal oleate desaturase genes (FAD2) from normal-type and high-oleic varieties of sunflower (Helianthus annuus L.). Mol Breed 8:159-168
6. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321-4325
7. Okuley J, Lightner J, Feldmann K, Yadav N, Lark E, Browse J (1994) Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis. Plant Cell 6:147-158
8. 12 fatty acid desaturase gene from the basidiomycete Lentinula edodes. Mol Genet Genomics 273:336-341
9. 12-fatty acid desaturase from arachidonic acid-producing Mortierella fungus by heterologous expression in the yeast Saccharomyces cerevisiae and the fungus Aspergillus oryzae. Eur J Biochem 261:812-820
10. Sambrook J, Fritsch EF, Maniatis T (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor, NY
11. Tonon T, Harvey D, Larson TR, Graham IA (2002) Long chain polyunsaturated fatty acid production and partitioning to triacylglycerols in four microalgae. Phytochemistry 61:15-24
12. 11-desaturase from the microalga Thalassiosira pseudonana. FEBS Lett 563:28-34
13. 12-fatty acid desaturase gene from Rhizopus arrhizus. FEBS Lett 573:45-50
14. 6-fatty acid desaturase from Rhizopus arrhizus. FEBS Lett 556:81-85
15. Zhang R, Zheng YF, Wu Y, Wang SH, Chen F (2006) A simple and efficient method for preparation of plant RNAs. Hereditas 28:583-584