Site-directed biotinylation of antibodies for controlled immobilization on solid surfaces

Analytical Biochemistry

Volumn 365, Issue 1, 2007, Pages 14-23

  Cho, Il Hoon ^a   Paek, Eui Hwan ^a   Lee, Haiwon ^b   Kang, Ji Yoon ^c   Kim, Tae Song ^c   Paek, Se Hwan ^a  

^a Korea University   (South Korea)

^b Hanyang University   (South Korea)

^c Korea Institute of Science and Technology   (South Korea)

Author keywords

Antibody fragmentation; Cardiac troponin I; Detection capability of immunoassay; Immobilization of antibody via streptavidin biotin linkage; Orientation of immobilized antibody

Indexed keywords

ACYLATION; ANTIBODIES; COENZYMES; HINGES;

BIOTIN-STREPTAVIDIN; CARDIAC TROPONIN I; DETECTION CAPABILITY; ENZYMATIC CLEAVAGE; IMMOBILIZED ANTIBODIES; OPTIMAL CONDITIONS; REAGENT CONCENTRATION; STREPTAVIDIN;

CHEMICAL DETECTION;

AMINE; ANTIBODY; BIOTIN; CARBOXYL GROUP; FICIN; IMMUNOGLOBULIN; IMMUNOGLOBULIN F(AB) FRAGMENT; MALEIMIDE; STREPTAVIDIN; TROPONIN;

ANTIBODY DETECTION; ANTIGEN ANTIBODY REACTION; ARTICLE; BIOTINYLATION; CONTROLLED STUDY; ENZYME ACTIVITY; FRAGMENTATION REACTION; HUMAN; IMMOBILIZATION; IMMUNOASSAY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; REDUCTION; SOLID; WESTERN BLOTTING;

EID: 34247381635     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.02.028     Document Type: Article

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