Esterase 2 from Alicyclobacillus acidocaldarius as a reporter and affinity tag for expression and single step purification of polypeptides

Protein Expression and Purification

Volumn 54, Issue 1, 2007, Pages 94-100

  Huang, Yiwei ^a   Humenik, Martin ^a   Sprinzl, Mathias ^a  

^a UNIVERSITY OF BAYREUTH   (Germany)

Author keywords

Affinity tag; Esterase; Reporter enzyme; Trifluoromethyl alkyl ketone

Indexed keywords

BLOOD CLOTTING FACTOR 10A; DRUG DERIVATIVE; ESTERASE; HYBRID PROTEIN; PEPTIDE; SEPHAROSE; SEPHAROSE CL 6B; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIUM; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS;

BACTERIA; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESTERASES; FACTOR XA; PEPTIDES; RECOMBINANT FUSION PROTEINS; SEPHAROSE;

ALICYCLOBACILLUS ACIDOCALDARIUS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; PROKARYOTA;

EID: 34247341377     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.02.005     Document Type: Article

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