Modification of an effector strain for replacement therapy of dental caries to enable clinical safety trials

Journal of Applied Microbiology

Volumn 102, Issue 5, 2007, Pages 1209-1219

  Hillman, J D ^a   Mo, J ^a   McDonell, E ^a   Cvitkovitch, D ^b   Hillman, C H ^c  

^a Oragenics Inc ^*  (United States)

^b UNIVERSITY OF TORONTO   (Canada)

^c UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

Dental caries; Molecular genetics; Replacement therapy; Safety trial

Indexed keywords

AMINO ACIDS; BIOCHEMISTRY; MOLECULAR BIOLOGY; RATS;

CLINICAL TRIAL; D ALANINES; DENTAL CARIES; GENETICALLY MODIFIED; IN-VITRO; MOLECULAR GENETICS; REPLACEMENT THERAPY; SAFETY TRIAL; STREPTOCOCCUS MUTANS; VIVO MODELS;

GENES;

A2JM; ANTIINFECTIVE AGENT; CHLORHEXIDINE; DEXTRO ALANINE; DNA; PROBIOTIC AGENT; STREPTOCOCCUS MUTANS A2JM; UNCLASSIFIED DRUG;

ANTIBIOTICS; DENTAL HEALTH; GENETIC ANALYSIS; MOLECULAR ANALYSIS; MUTATION; SAFETY;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; BACTERIAL COLONIZATION; BACTERIAL GENE; BACTERIAL STRAIN; CONFERENCE PAPER; CONTROLLED STUDY; DELETION MUTANT; DENTAL CARIES; DNA MODIFICATION; ERADICATION THERAPY; FEMALE; GENE CONSTRUCT; GENE DELETION; INFECTION PREVENTION; MALE; NONHUMAN; OPEN READING FRAME; RAT; STOMACH LAVAGE; STREPTOCOCCUS MUTANS; THERAPY EFFECT; TREATMENT OUTCOME; TREATMENT RESPONSE;

ANIMALIA; BACTERIA (MICROORGANISMS); RATTUS; STREPTOCOCCUS MUTANS;

EID: 34247331771     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2007.03316.x     Document Type: Conference Paper

References (17)

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.E., Seidman, J.G., Smith, J.A. Struhl, K. (1997) Short Protocols in Molecular Biology. London, England: John Wiley & Sons, Inc.
2. Chen, A., Hillman, J.D. Duncan, M. (1994) L(+)-lactate dehydrogenase deficiency is lethal in Streptococcus mutans. J Bacteriol 176, 1542 1545.
3. Gutierrez, J.A., Crowley, P.J., Brown, D.P., Hillman, J.D., Youngman, P. Bleiweis, A.S. (1996) Insertional mutagenesis and recovery of interrupted genes of Streptococcus mutans using transposon Tn917: preliminary characterization of mutants displaying acid sensitivity and nutritional requirements. J Bacteriol 178, 4166 4175.
4. Heaton, M.P., Johnston, R.B. Thompson, T.L. (1988) Controlled lysis of bacterial cells utilizing mutants with defective synthesis of d-alanine. Can J Microbiol 34, 256 261.
5. Hillman, J.D. (2002) Genetically modified Streptococcus mutans for the prevention of dental caries. Antonie van Leeuwenhoek 82, 361 366.
6. Hillman, J.D., Dzuback, A.L. Andrews, S.W. (1987) Colonization of the human oral cavity by a Streptococcus mutans mutant producing increased bacteriocin. J Dent Res 66, 1092 1094.
7. Hillman, J.D., Chen, A., Duncan, M. Lee, S.-W. (1994) Evidence that L(+)-lactate dehydrogenase deficiency is lethal in Streptococcus mutans. Infect Immun 62, 60 64.
8. Hillman, J.D., Chen, A. Snoop, J. (1996) Genetic and physiological analysis of LDH-deficiency lethality in Streptococcus mutans: complementation by alcohol dehydrogenase from Zymomonas mobilis. Infect Immun 64, 4319 4323.
9. Hillman, J.D., Novák, J., Sagura, E., Gutierrez, J.A., Brooks, T.A., Crowley, P.J., Azziz, A., Leung, K.-P., et al. (1998) Genetic and biochemical analysis of mutacin 1140, a lantibiotic from Streptococcus mutans. Infect Immun 66, 2743 2749.
10. Hillman, J.D., Brooks, T.A., Michalek, S.M., Harmon, C.C., Snoep, J.L. van der Weijden, C.C. (2000) Construction and characterization of an effector strain of Streptococcus mutans for the replacement therapy of dental caries. Infect Immun 68, 543 549.
11. Inagaki, K., Tanizawa, K., Badet, B., Walsh, C.T., Tanaka, H. Soda, K. (1986) Thermostable alanine racemase from Bacillus stearothermophilus: molecular cloning of the gene, enzyme purification, and characterization. Biochemistry 25, 3268 3274.
12. Lee, S.F., Progulske-Fox, A., Erdos, G.W., Piacentini, D.A., Ayakawa, G.Y., Crowley, P.J. Bleiweis, A.S. (1989) Construction and characterization of isogenic mutants of Streptococcus mutans deficient in major surface protein antigen P1 (I/II). Infect Immun 57, 3306 3313.
13. Li, Y.H., Lau, P.C., Lee, J.H., Ellen, R.P. Cvitkovitch, D.G. (2001) Natural genetic transformation of Streptococcus mutans growing in biofilms. J Bacteriol 183, 897 908.
14. Macrina, F.L., Jones, K.R. Wood, P.H. (1980) Chimeric streptococcal plasmids and their use as molecular cloning vehicles in Streptococcus sanguis (Challis). J Bacteriol 143, 1425 1435.
15. Nagata, Y., Higashi, M., Ishii, Y., Sano, H., Tanigawa, M., Nagata, K., Noguchi, K. Urade, M. (2006) Life Sci 78, 1677 1681.
16. Oral Health in America: A Report of the Surgeon General (2000) National Institute of Dental and Craniofacial Research (U.S.). Dept. of Health and Human Services, U.S. Public Health Service, Rockville, Md.
17. Wasserman, S.A., Walsh, C.T. Botstein, D. (1983) Two alanine racemase genes in Salmonella typhimurium that differ in structure and function. J Bacteriol 153, 1439 1450.