Characterization of physical and functional anchor site interactions in human telomerase

Molecular and Cellular Biology

Volumn 27, Issue 8, 2007, Pages 3226-3240

  Wyatt, Haley D M ^a   Lobb, Deirdre A ^a   Beattie, Tara L ^a,b  

^a UNIVERSITY OF CALGARY   (Canada)

^b Southern Alberta Cancer Research Institute   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; DNA; PRIMER DNA; PROTEIN SUBUNIT; RNA; RNA DIRECTED DNA POLYMERASE; TELOMERASE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING ASSAY; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME SUBUNIT; GENE TRANSLOCATION; HUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DNA INTERACTION; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; SEQUENCE ANALYSIS; TELOMERE;

AMINO ACIDS; BASE SEQUENCE; BIOTINYLATION; DNA; DNA PRIMERS; HUMANS; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDES; PROTEIN BINDING; RNA; TELOMERASE; TELOMERE;

EID: 34147194788     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.02368-06     Document Type: Article

References (52)

1. Armbruster, B. N., S. S. Banik, C. Guo, A. C. Smith, and C. M. Counter. 2001. N-terminal domains of the human telomerase catalytic subunit required for enzyme activity in vivo. Mol. Cell. Biol. 21:7775-7786.
2. Armbruster, B. N., K. T. Etheridge, D. Broccoli, and C. M. Counter. 2003. Putative telomere-recruiting domain in the catalytic subunit of human telomerase. Mol. Cell. Biol. 23:3237-3246.
3. Armbruster, B. N., C. M. Linardic, T. Veldman, N. P. Bansal, D. L. Downie, and C. M. Counter. 2004. Rescue of an hTERT mutant defective in telomere elongation by fusion with hPot1. Mol. Cell. Biol. 24:3552-3561.
4. Autexier, C., and N. F. Lue. 2006. The structure and function of telomerase reverse transcriptase. Annu. Rev. Biochem. 75:493-517.
5. Bachand, F., and C. Autexier. 2001. Functional regions of human telomerase reverse transcriptase and human telomerase RNA required for telomerase activity and RNA-protein interactions. Mol. Cell. Biol. 21:1888-1897.
6. Bailey, S. M., and J. P. Murnane. 2006. Telomeres, chromosome instability and cancer. Nucleic Acids Res. 34:2408-2417.
7. Banik, S. S., C. Guo, A. C. Smith, S. S. Margolis, D. A. Richardson, C. A. Tirado, and C. M. Counter. 2002. C-terminal regions of the human telomerase catalytic subunit essential for in vivo enzyme activity. Mol. Cell. Biol. 22:6234-6246.
8. Baran, N., Y. Haviv, B. Paul, and H. Manor. 2002. Studies on the minimal lengths required for DNA primers to be extended by the Tetrahymena telomerase: implications for primer positioning by the enzyme. Nucleic Acids Res. 30:5570-5578.
9. Beattie, T. L., W. Zhou, M. O. Robinson, and L. Harrington. 2001. Functional multimerization of the human telomerase reverse transcriptase. Mol. Cell. Biol. 21:6151-6160.
10. Beattie, T. L., W. Zhou, M. O. Robinson, and L. Harrington. 2000. Polymerization defects within human telomerase are distinct from telomerase RNA and TEP1 binding. Mol. Biol. Cell 11:3329-3340.
11. Beattie, T. L., W. Zhou, M. O. Robinson, and L. Harrington. 1998. Reconstitution of human telomerase activity in vitro. Curr. Biol. 8:177-180.
12. Bosoy, D., and N. F. Lue. 2001. Functional analysis of conserved residues in the putative "finger" domain of telomerase reverse transcriptase. J. Biol. Chem. 276:46305-46312.
13. Bryan, T. M., K. J. Goodrich, and T. R. Cech. 2000. A mutant of Tetrahymena telomerase reverse transcriptase with increased processivity. J. Biol. Chem. 275:24199-24207.
14. Collins, K., and C. W. Greider. 1993. Tetrahymena telomerase catalyzes nucleolytic cleavage and nonprocessive elongation. Genes Dev. 7:1364-1376.
15. Fletcher, T. M., D. Sun, M. Salazar, and L. H. Hurley. 1998. Effect of DNA secondary structure on human telomerase activity. Biochemistry 37:5536-5541.
16. Gilley, D., H. Tanaka, and B. S. Herbert. 2005. Telomere dysfunction in aging and cancer. Int. J. Biochem. Cell Biol. 37:1000-1013.
17. Greider, C. W. 1991. Telomerase is processive. Mol. Cell. Biol. 11:4572-4580.
18. Greider, C. W. 1996. Telomere length regulation. Annu. Rev. Biochem. 65:337-365.
19. Greider, C. W., and E. H. Blackburn. 1985. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell 43:405-413.
20. Greider, C. W., and E. H. Blackburn. 1987. The telomere terminal transferase of Tetrahymena is a ribonucleoprotein enzyme with two kinds of primer specificity. Cell 51:887-898.
21. Greider, C. W., and E. H. Blackburn. 1989. A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis. Nature 337:331-337.
22. Hainaut, P., A. Hall, and J. Milner. 1994. Analysis of p53 quaternary structure in relation to sequence-specific DNA binding. Oncogene 9:299-303.
23. Hammond, P. W., T. N. Lively, and T. R. Cech. 1997. The anchor site of telomerase from Euplotes aediculatus revealed by photo-cross-linking to single- and double-stranded DNA primers. Mol. Cell. Biol. 17:296-308.
24. Hardy, C. D., C. S. Schultz, and K. Collins. 2001. Requirements for the dGTP-dependent repeat addition processivity of recombinant Tetrahymena telomerase. J. Biol. Chem. 276:4863-4871.
25. Harrington, L., W. Zhou, T. McPhail, R. Oulton, D. S. Yeung, V. Mar, M. B. Bass, and M. O. Robinson. 1997. Human telomerase contains evolutionarily conserved catalytic and structural subunits. Genes Dev. 11:3109-3115.
26. Harrington, L. A., and C. W. Greider. 1991. Telomerase primer specificity and chromosome healing. Nature 353:451-454.
27. Hossain, S., S. Singh, and N. F. Lue. 2002. Functional analysis of the C-terminal extension of telomerase reverse transcriptase: a putative "thumb" domain. J. Biol. Chem. 277:36174-36180.
28. Huard, S., T. J. Moriarty, and C. Autexier. 2003. The C terminus of the human telomerase reverse transcriptase is a determinant of enzyme processivity. Nucleic Acids Res. 31:4059-4070.
29. Jacobs, S. A., E. R. Podell, and T. R. Cech. 2006. Crystal structure of the essential N-terminal domain of telomerase reverse transcriptase. Nat. Struct. Mol. Biol. 13:218-225.
30. Jacques, P. S., B. M. Wohrl, M. Ottmann, J. L. Darlix, and S. F. Le Grice. 1994. Mutating the "primer grip" of p66 HIV-1 reverse transcriptase implicates tryptophan-229 in template-primer utilization. J. Biol. Chem. 269: 26472-26478.
31. Ji, H., M. H. Platts, L. M. Dharamsi, and K. L. Friedman. 2005. Regulation of telomere length by an N-terminal region of the yeast telomerase reverse transcriptase. Mol. Cell. Biol. 25:9103-9114.
32. Kelleher, C., M. T. Teixeira, K. Forstemann, and J. Lingner. 2002. Telomerase: biochemical considerations for enzyme and substrate. Trends Biochem. Sci. 27:572-579.
33. Lee, M. S., and E. H. Blackburn. 1993. Sequence-specific DNA primer effects on telomerase polymerization activity. Mol. Cell. Biol. 13:6586-6599.
34. Lee, S. R., J. M. Wong, and K. Collins. 2003. Human telomerase reverse transcriptase motifs required for elongation of a telomeric substrate. J. Biol. Chem. 278:52531-52536.
35. Lingner, J., T. R. Hughes, A. Shevchenko, M. Mann, V. Lundblad, and T. R. Cech. 1997. Reverse transcriptase motifs in the catalytic subunit of telomerase. Science 276:561-567.
36. Lue, N. F. 2005. A physical and functional constituent of telomerase anchor site. J. Biol. Chem. 280:26586-26591.
37. Lue, N. F., Y. C. Lin, and I. S. Mian. 2003. A conserved telomerase motif within the catalytic domain of telomerase reverse transcriptase is specifically required for repeat addition processivity. Mol. Cell. Biol. 23:8440-8449.
38. Meyerson, M., C. M. Counter, E. N. Eaton, L. W. Ellisen, P. Steiner, S. D. Caddle, L. Ziaugra, R. L. Beijersbergen, M. J. Davidoff, Q. Liu, S. Bacchetti, D. A. Haber, and R. A. Weinberg. 1997. hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Cell 90:785-795.
39. Moriarty, T. J., S. Huard, S. Dupuis, and C. Autexier. 2002. Functional multimerization of human telomerase requires an RNA interaction domain in the N terminus of the catalytic subunit. Mol. Cell. Biol. 22:1253-1265.
40. Moriarty, T. J., D. T. Marie-Egyptlenne, and C. Autexier. 2004. Functional organization of repeat addition processivity and DNA synthesis determinants in the human telomerase multimer. Mol. Cell. Biol. 24:3720-3733.
41. Moriarty, T. J., R. J. Ward, M. A. Taboski, and C. Autexier. 2005. An anchor site-type defect in human telomerase that disrupts telomere length maintenance and cellular immortalization. Mol. Biol. Cell 16:3152-3161.
42. Morin, G. B. 1989. The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats. Cell 59:521-529.
43. Morin, G. B. 1991. Recognition of a chromosome truncation site associated with alpha-thalassaemia by human telomerase. Nature 353:454-456.
44. Nakamura, T. M., G. B. Morin, K. B. Chapman, S. L. Weinrich, W. H. Andrews, J. Lingner, C. B. Harley, and T. R. Cech. 1997. Telomerase catalytic subunit homologs from fission yeast and human. Science 277:955-959.
45. Opresko, P. L., P. A. Mason, E. R. Podell, M. Lei, I. D. Hickson, T. R. Cech, and V. A. Bohr. 2005. POT1 stimulates RecQ helicases WRN and BLM to unwind telomeric DNA substrates. J. Biol. Chem. 280:32069-32080.
46. Peng, Y., I. S. Mian, and N. F. Lue. 2001. Analysis of telomerase processivity: mechanistic similarity to HIV-1 reverse transcriptase and role in telomere maintenance. Mol. Cell 7:1201-1211.
47. Smogorzewska, A., and T. de Lange. 2004. Regulation of telomerase by telomeric proteins. Annu. Rev. Biochem. 73:177-208.
48. Snow, B. E., N. Erdmann, J. Cruickshank, H. Goldman, R. M. Gill, M. O. Robinson, and L. Harrington. 2003. Functional conservation of the telomerase protein Est1p in humans. Curr. Biol. 13:698-704.
49. Wallweber, G., S. Gryaznov, K. Pongracz, and R. Pruzan. 2003. Interaction of human telomerase with its primer substrate. Biochemistry 42:589-600.
50. Weinrich, S. L., R. Pruzan, L. Ma, M. Ouellette, V. M. Tesmer, S. E. Holt, A. G. Bodnar, S. Lichtsteiner, N. W. Kim, J. B. Trager, R. D. Taylor, R. Carlos, W. H. Andrews, W. E. Wright, J. W. Shay, C. B. Harley, and G. B. Morin. 1997. Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT. Nat. Genet. 17: 498-502.
51. Xia, J., Y. Peng, I. S. Mian, and N. F. Lue. 2000. Identification of functionally important domains in the N-terminal region of telomerase reverse transcriptase. Mol. Cell. Biol. 20:5196-5207.
52. Zaug, A. J., E. R. Podell, and T. R. Cech. 2005. Human POT1 disrupts telomeric G-quadruplexes allowing telomerase extension in vitro. Proc. Natl. Acad. Sci. USA 102:10864-10869.