Expression in Pichia pastoris and immunological evaluation of a truncated Dengue envelope protein

Molecular Biotechnology

Volumn 35, Issue 1, 2007, Pages 23-30

  Valdés, Iris ^a   Hermida, Lisset ^a   Zulueta, Aída ^a   Martín, Jorge ^a   Silva, Ricardo ^a   Álvarez, Mayling ^b   Guzmán, María G ^b   Guillén, Gerardo ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

^b INSTITUTO DE MEDICINA TROPICAL PEDRO KOURÍ   (Cuba)

Author keywords

Dengue virus; Expression; Glycosylation; Pichia pastoris yeast; Secretion

Indexed keywords

ANTIGENIC CHARACTERISTICS; DENGUE VIRUS; GLYCOSYLATED PROTEINS; PICHIA PASTORIS YEAST;

ANTIBODIES; ANTIGENS; GENE EXPRESSION; GLYCOSYLATION; VIRUSES;

DISEASES;

ALCOHOL OXIDASE; ALCOHOL OXIDASE 1; BETA FRUCTOFURANOSIDASE; GLYCOPROTEIN E2; GLYCOSYLATED PROTEIN; RECOMBINANT PROTEIN; SIGNAL PEPTIDE; UNCLASSIFIED DRUG; VIRUS ANTIBODY;

ACTIVE IMMUNIZATION; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY PRODUCTION; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DENGUE VIRUS; FEMALE; GENE EXPRESSION SYSTEM; IMMUNOASSAY; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; PROTEIN CONFORMATION; PROTEIN FOLDING; SACCHAROMYCES CEREVISIAE; SOLUBILITY;

ANIMALS; ANTIBODIES, VIRAL; ANTIGENS, VIRAL; BASE SEQUENCE; BETA-FRUCTOFURANOSIDASE; BIOREACTORS; BIOTECHNOLOGY; CLONING, MOLECULAR; DENGUE VIRUS; DNA, VIRAL; FEMALE; GENE EXPRESSION; GENES, FUNGAL; GENES, VIRAL; GLYCOSYLATION; IMMUNIZATION; MICE; MICE, INBRED BALB C; PEPTIDE FRAGMENTS; PICHIA; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; VIRAL ENVELOPE PROTEINS;

DENGUE VIRUS; DENGUE VIRUS TYPE 2; MUS; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 34147176616     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:35:1:23     Document Type: Article

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