A novel method for reproducible fluorescent labeling of small amounts of antibodies on solid phase

Journal of Immunological Methods

Volumn 322, Issue 1-2, 2007, Pages 40-49

  Lundberg, Emma ^a   Sundberg, Mårten ^a   Gräslund, Torbjörn ^a   Uhlén, Mathias ^a   Svahn, Helene Andersson ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

Author keywords

Alexa fluor 555; Alexa fluor 647; Antibody; Labeling; Protein A; Solid phase

Indexed keywords

ANTIBODY; FLUORESCENT DYE; PROTEIN A;

ANTIBODY LABELING; ARTICLE; BINDING AFFINITY; CONCENTRATION RESPONSE; CONJUGATION; COVALENT BOND; CYTOLOGY; FLUORESCENCE ANALYSIS; HUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; QUANTITATIVE ANALYSIS; REACTION OPTIMIZATION; REPRODUCIBILITY; SOLID;

ANTIBODIES; CYCLIC AMP; FLUORESCENT DYES; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOHISTOCHEMISTRY; PROTEIN ARRAY ANALYSIS; REPRODUCIBILITY OF RESULTS; STAINING AND LABELING;

ALEXA;

EID: 34147096033     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2007.01.023     Document Type: Article

References (11)

1. Berlier J.E., Rothe A., Buller G., Bradford J., Gray D.R., Filanoski B.J., Telford W.G., Yue S., Liu J., Cheung C.-Y., Chang W., Hirsch J.D., Beechem J.M., Haugland R.P., and Haugland R.P. Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates. J. Histochem. Cytochem. 51 (2003) 1699
2. Gruber H.J., Hahn C.D., Kada G., Riener C.K., Harms G.S., Ahrer W., Dax T.G., and Knaus H.-G. Anomalous fluorescence enhancement of Cy3 and Cy3.5 versus anomalous fluorescence loss of Cy5 and Cy7 upon covalent linking to IgG and noncovalent binding to avidin. Bioconjug. Chem. 11 (2000) 696
3. Haugland R. In: Spence M.T.Z. (Ed). The Handbook - A Guide to Fluorescent Probes and Labeling Technologies. (2005), Invitrogen Molecular Probes, Eugene, OR
4. Langone J.J. Applications of immobilized protein A in immunochemical techniques. J. Immunol. Methods 55 (1982) 277
5. Mujumdar R., Ernst L., Mujumdar S., Lewis C., and Waggoner A. Cyanine dye labeling reagents: sulfoindocyanine succinimidyl esters. Bioconjug. Chem. 4 (1993) 105
6. Nilsson P., Paavilainen L., Larsson K., Ödling J., Sundberg M., Andersson A.-C., Kampf C., Persson A., Al-Khalili Szigyarto C., Ottosson J., Björling E., Hober S., Wernérus H., Wester K., Pontén F., and Uhlen M. Towards a human proteome atlas: high-throughput generation of mono-specific antibodies for tissue profiling. Proteomics 5 (2005) 4327
7. Panchuk-Voloshina N., Haugland R.P., Bishop-Stewart J., Bhalgat M.K., Millard P.J., Mao F., Leung W.-Y., and Haugland R.P. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J. Histochem. Cytochem. 47 (1999) 1179
8. Strachan E.A., Mallia K., Cox J.M., Antharavally B., Desai S., Sykaluk L., O'Sullivan V., and Bell P.A. Solid-phase biotinylation of antibodies. J. Mol. Recognit. 17 (2004) 268
9. Uhlen M., Bjorling E., Agaton C., Szigyarto C.A.-K., Amini B., Andersen E., Andersson A.-C., Angelidou P., Asplund A., Asplund C., Berglund L., Bergstrom K., Brumer H., Cerjan D., Ekstrom M., Elobeid A., Eriksson C., Fagerberg L., Falk R., Fall J., Forsberg M., Bjorklund M.G., Gumbel K., Halimi A., Hallin I., Hamsten C., Hansson M., Hedhammar M., Hercules G., Kampf C., Larsson K., Lindskog M., Lodewyckx W., Lund J., Lundeberg J., Magnusson K., Malm E., Nilsson P., Odling J., Oksvold P., Olsson I., Oster E., Ottosson J., Paavilainen L., Persson A., Rimini R., Rockberg J., Runeson M., Sivertsson A., Skollermo A., Steen J., Stenvall M., Sterky F., Stromberg S., Sundberg M., Tegel H., Tourle S., Wahlund E., Walden A., Wan J., Wernerus H., Westberg J., Wester K., Wrethagen U., Xu L.L., Hober S., and Ponten F. A human protein atlas for normal and cancer tissues based on antibody proteomics. Mol. Cell. Proteomics 4 (2005) 1920
10. Uhlen M., and Ponten F. Antibody-based proteomics for human tissue profiling. Mol. Cell. Proteomics 4 (2005) 384
11. Warford A., Howat W., and McCafferty J. Expression profiling by high-throughput immunohistochemistry. J. Immunol. Methods 290 (2004) 81