Optimization of culture conditions for porcine corneal endothelial cells

Molecular Vision

Volumn 13, Issue , 2007, Pages 524-533

  Proulx, Stéphanie ^a   Bourget, Jean Michel ^a   Gagnon, Nicolas ^a   Martel, Sophie ^a   Deschambeault, Alexandre ^a   Carrier, Patrick ^a   Giasson, Claude J ^a,b   Auger, François A ^a   Brunette, Isabelle ^c   Germain, Lucie ^a  

^a LAVAL UNIVERSITY   (Canada)

^b UNIVERSITÉ DE MONTRÉAL   (Canada)

^c MAISONNEUVE ROSEMONT HOSPITAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ASCORBIC ACID; BROXURIDINE; CHONDROITIN SULFATE; EPIDERMAL GROWTH FACTOR; HYPOPHYSIS EXTRACT; NERVE GROWTH FACTOR;

ANIMAL CELL; ARTICLE; CELL COUNT; CELL CULTURE; CELL DENSITY; CELL GROWTH; CELL POPULATION; CELL PROLIFERATION; CELL SHAPE; CELL SIZE; CELL STRAIN 3T3; CELL STRUCTURE; COCULTURE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CORNEA ENDOTHELIUM; CULTURE MEDIUM; CULTURE TECHNIQUE; ENDOTHELIUM CELL; MONOLAYER CULTURE; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; SWINE;

3T3 CELLS; ANIMALS; ASCORBIC ACID; CATTLE; CELL COUNT; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELL SHAPE; CELLS, CULTURED; CHONDROITIN SULFATES; COCULTURE TECHNIQUES; CULTURE MEDIA; DOSE-RESPONSE RELATIONSHIP, DRUG; ENDOTHELIUM, CORNEAL; FETAL BLOOD; MICE; PITUITARY GLAND; SWINE; TISSUE EXTRACTS;

EID: 34047246516     PISSN: None     EISSN: 10900535     Source Type: Journal    
DOI: None     Document Type: Article

References (59)

1. Joyce NC, Meklir B, Joyce SJ, Zieske JD. Cell cycle protein expression and proliferative status in human corneal cells. Invest Ophthalmol Vis Sci 1996; 37:645-55.
2. Joyce NC, Navon SE, Roy S, Zieske JD. Expression of cell cycle-associated proteins in human and rabbit corneal endothelium in situ. Invest Ophthalmol Vis Sci 1996; 37:1566-75.
3. Joyce NC. Cell cycle status in human corneal endothelium. Exp Eye Res 2005; 81:629-38.
4. Wilson SE, Lloyd SA, He YG, McCash CS. Extended life of human corneal endothelial cells transfected with the SV40 large T antigen. Invest Ophthalmol Vis Sci 1993; 34:2112-23.
5. Wilson SE, Weng J, Blair S, He YG, Lloyd S. Expression of E6/E7 or SV40 large T antigen-coding oncogenes in human corneal endothelial cells indicates regulated high-proliferative capacity. Invest Ophthalmol Vis Sci 1995; 36:32-40.
6. Engelmann K, Bohnke M, Friedl P. Isolation and long-term cultivation of human corneal endothelial cells. Invest Ophthalmol Vis Sci 1988; 29:1656-62.
7. Engelmann K, Friedl P. Optimization of culture conditions for human corneal endothelial cells. In Vitro Cell Dev Biol 1989; 25:1065-72.
8. Engelmann K, Friedl P. Growth of human corneal endothelial cells in a serum-reduced medium. Cornea 1995; 14:62-70.
9. Bednarz J, Doubilei V, Wollnik PC, Engelmann K. Effect of three different media on serum free culture of donor corneas and isolated human corneal endothelial cells. Br J Ophthalmol 2001; 85:1416-20.
10. Zhu C, Joyce NC. Proliferative response of corneal endothelial cells from young and older donors. Invest Ophthalmol Vis Sci 2004; 45:1743-51.
11. Joyce NC, Zhu CC. Human corneal endothelial cell proliferation: potential for use in regenerative medicine. Cornea 2004; 23:S8-S19.
12. Gospodarowicz D, Mescher AL, Birdwell CR. Stimulation of corneal endothelial cell proliferations in vitro by fibroblast and epidermal growth factors. Exp Eye Res 1977; 25:75-89.
13. Giguere L, Cheng J, Gospodarowicz D. Factors involved in the control of proliferation of bovine corneal endothelial cells maintained in serum-free medium. J Cell Physiol 1982; 110:72-80.
14. Lee HJ, Lin CP, Chen CW. The effects of epidermal growth factor and chondroitin sulfate on the animal corneal endothelial cell culture. Gaoxiong Yi Xue Ke Xue Za Zhi 1991; 7:614-21.
15. Schultz G, Cipolla L, Whitehouse A, Eiferman R, Woost P, Jumblatt M. Growth factors and corneal endothelial cells: III. Stimulation of adult human corneal endothelial cell mitosis in vitro by defined mitogenic agents. Cornea 1992; 11:20-7.
16. Woost PG, Jumblatt MM, Eiferman RA, Schultz GS. Growth factors and corneal endothelial cells: II. Characterization of epidermal growth factor receptor from bovine corneal endothelial cells. Cornea 1992; 11:11-9.
17. Woost PG, Jumblatt MM, Eiferman RA, Schultz GS. Growth factors and corneal endothelial cells: I. Stimulation of bovine corneal endothelial cell DNA synthesis by defined growth factors. Cornea 1992; 11:1-10.
18. Samples JR, Binder PS, Nayak SK. Propagation of human corneal endothelium in vitro effect of growth factors. Exp Eye Res 1991; 52:121-8.
19. Pond WG, Houpt KA. The Biology of the Pig. Ithaca (NY): Comstock Publishing; 1978.
20. Chang CH, Lin CP, Wang HZ. A tetrazolium-based(MTT) assay for measuring viability of cultured porcine corneal endothelium. Gaoxiong Yi Xue Ke Xue Za Zhi 1993; 9:610-5.
21. Wang HZ, Chang CH, Lin CP, Tsai MC. Using MTT viability assay to test the cytotoxicity of antibiotics and steroid to cultured porcine corneal endothelial cells. J Ocul Pharmacol Ther 1996; 12:35-43.
22. Schneider AI, Maier-Reif K, Graeve T. Constructing an in vitro cornea from cultures of the three specific corneal cell types. In Vitro Cell Dev Biol Anim 1999; 35:515-26.
23. Sobottka Ventura AC, Engelmann K, Bohnke M. Fetal calf serum protects cultured porcine corneal endothelial cells from endotoxin-mediated cell damage. Ophthalmic Res 1999; 31:416-25.
24. Wu KY, Hong SJ, Huang HT, Lin CP, Chen CW. Toxic effects of mitomycin-C on cultured corneal keratocytes and endothelial cells. J Ocul Pharmacol Ther 1999; 15:401-11.
25. Wusteman M, Wang LH. Some observations on the use of cultured corneal endothelial cells as a model for intact corneal endothelium. Cryobiology 2000; 40:376-80.
26. Wang HZ, Hong SJ, Wu KY. Change of calcium and cAMP concentration by adrenoceptor agents in cultured porcine corneal endothelial cells. J Ocul Pharmacol Ther 2000; 16:299-309.
27. Wollensak G, Sporl E, Reber F, Pillunat L, Funk R. Corneal endothelial cytotoxicity of riboflavin/UVA treatment in vitro. Ophthalmic Res 2003; 35:324-8.
28. Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 1975; 6:331-43.
29. Green H. Regeneration of the skin after grafting of epidermal cultures. Lab Invest 1989; 60:583-4.
30. Germain L, Auger FA. Tissue engineered biomaterials: biological and mechanical characteristics. In: Encyclopedic Handbook of Biomaterials and Bioengineering, part B; Applications. New York, USA: Marcel Dekker, inc. Publishers; 1995. p. 699-734.
31. Michel M, L'Heureux N, Pouliot R, Xu W, Auger FA, Germain L. Characterization of a new tissue-engineered human skin equivalent with hair. In Vitro Cell Dev Biol Anim 1999; 35:318-26.
32. Lindberg K, Brown ME, Chaves HV, Kenyon KR, Rheinwald JG. In vitro propagation of human ocular surface epithelial cells for transplantation. Invest Ophthalmol Vis Sci 1993; 34:2672-9.
33. Germain L, Auger FA, Grandbois E, Guignard R, Giasson M, Boisjoly H, Guerin SL. Reconstructed human cornea produced in vitro by tissue engineering. Pathobiology 1999; 67:140-7.
34. Rama P, Bonini S, Lambiase A, Golisano O, Paterna P, De Luca M, Pellegrini G. Autologous fibrin-cultured limbal stem cells permanently restore the corneal surface of patients with total limbal stem cell deficiency. Transplantation 2001; 72:1478-85.
35. Nakamura T, Koizumi N, Tsuzuki M, Inoki K, Sano Y, Sotozono C, Kinoshita S. Successful regrafting of cultivated corneal epithelium using amniotic membrane as a carrier in severe ocular surface disease. Cornea 2003; 22:70-1.
36. Talbot M, Carrier P, Giasson CJ, Deschambeault A, Guerin SL, Auger FA, Bazin R, Germain L. Autologous transplantation of rabbit limbal epithelia cultured on fibrin gels for ocular surface reconstruction. Mol Vis 2006; 12:65-75.
37. van Erp PE, Brons PP, Boezeman JB, de Jongh GJ, Bauer FW. A rapid flow cytometric method for bivariate bromodeoxyuridine/ DNA analysis using simultaneous proteolytic enzyme digestion and acid denaturation. Cytometry 1988; 9:627-30.
38. Stocker FW, Eiring A, Georgiade R, Georgiade N. A tissue culture technique for growing corneal epithelial, stromal, and endothelial tissues separately. Am J Ophthalmol 1958; 46:294-8.
39. Yu BY, Sugar J, Gilboy JE, Elvart JL. Growth of human corneal endothelial cells in culture. Invest Ophthalmol Vis Sci 1989; 30:248-53.
40. Barrandon Y, Green H. Three clonal types of keratinocyte with different capacities for multiplication. Proc Natl Acad Sci U S A 1987; 84:2302-6.
41. Couch JM, Cullen P, Casey TA, Fabre JW. Mitotic activity of corneal endothelial cells in organ culture with recombinant human epidermal growth factor. Ophthalmology 1987; 94:1-6.
42. Hoppenreijs VP, Pels E, Vrensen GF, Oosting J, Treffers WF. Effects of human epidermal growth factor on endothelial wound healing of human corneas. Invest Ophthalmol Vis Sci 1992; 33:1946-57.
43. Raymond GM, Jumblatt MM, Bartels SP, Neufeld AH. Rabbit corneal endothelial cells in vitro: effects of EGF. Invest Ophthalmol Vis Sci 1986; 27:474-9.
44. Nayak SK, Samples JR, Deg JK, Binder PS. Growth characteristics of primate (baboon) corneal endothelium in vitro. Invest Ophthalmol Vis Sci 1986; 27:607-11.
45. Nayak SK, Binder PS. The growth of endothelium from human corneal rims in tissue culture. Invest Ophthalmol Vis Sci 1984; 25:1213-6.
46. Yue BY, Niedra R, Baum JL. Effects of ascorbic acid on cultured rabbit corneal endothelial cells. Invest Ophthalmol Vis Sci 1980; 19:1471-6.
47. Shelton DL, Reichardt LF. Studies on the regulation of beta-nerve growth factor gene expression in the rat iris: the level of mRNA-encoding nerve growth factor is increased in irises placed in explant cultures in vitro, but not in irises deprived of sensory or sympathetic innervation in vivo. J Cell Biol 1986; 102:1940-8.
48. Lambiase A, Bonini S, Manni L, Ghinelli E, Tirassa P, Rama P, Aloe L. Intraocular production and release of nerve growth factor after iridectomy. Invest Ophthalmol Vis Sci 2002; 43:2334-40.
49. Lambiase A, Bonini S, Micera A, Rama P, Bonini S, Aloe L. Expression of nerve growth factor receptors on the ocular surface in healthy subjects and during manifestation of inflammatory diseases. Invest Ophthalmol Vis Sci 1998; 39:1272-5.
50. Klein R, Jing SQ, Nanduri V, O'Rourke E, Barbacid M. The trk proto-oncogene encodes a receptor for nerve growth factor. Cell 1991; 65:189-97.
51. Kent KD, Bomser JA. Bovine pituitary extract provides remarkable protection against oxidative stress in human prostate epithelial cells. In Vitro Cell Dev Biol Anim 2003; 39:388-94.
52. Praus R, Brettschneider I. Glycosaminoglycans in embryonic and postnatal human cornea. Ophthalmic Res 1975; 7:542.
53. Kaufman HE, Varnell ED, Kaufman S. Chondroitin sulfate in a new cornea preservation medium. Am J Ophthalmol 1984; 98:112-4.
54. Bourne WM, Lindstrom RL, Doughman DJ. Endothelial cell survival on transplanted human corneas preserved by organ culture with 1.35% chondroitin sulfate. Am J Ophthalmol 1985; 100:789-93.
55. Stein RM, Bourne WM, Campbell RJ. Chondroitin sulfate for corneal preservation at 4 degrees C. Evaluation by electron microscopy. Arch Ophthalmol 1986; 104:1358-61.
56. Lindstrom RL, Doughman DJ, Skelnik DL, Mindrup EA. Minnesota system corneal preservation. Br J Ophthalmol 1986; 70:47-54.
57. Stein RM, Laibson PR. Comparison of chondroitin sulfate to McCarey-Kaufman medium for corneal storage. Am J Ophthalmol 1987; 104:490-3.
58. Lin CP, Bohnke M, Draeger J. Effects of fibroblast growth factor and chondroitin sulfate on predamaged corneal endothelium. An organ culture study. Ophthalmic Res 1990; 22:173-7.
59. Hennings H, Michael D, Cheng C, Steinert P, Holbrook K, Yuspa SH. Calcium regulation of growth and differentiation of mouse epidermal cells in culture. Cell 1980; 19:245-54.