Inhibitors of bacterial virulence identified in a surrogate host model

Cellular Microbiology

Volumn 9, Issue 5, 2007, Pages 1336-1342

  Benghezal, Mohammed ^a   Adam, Eric ^a,b,c   Lucas, Aurore ^a   Burn, Christine ^a   Orchard, Michael G ^a   Deuschel, Christine ^a   Valentino, Emilio ^a   Braillard, Stéphanie ^a   Paccaud, Jean Pierre ^a   Cosson, Pierre ^b  

^a Athelas SA   (Switzerland)

^b UNIVERSITY OF GENEVA MEDICAL SCHOOL   (Switzerland)

^c CREALYS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

3 (ALLYLSULFANYL) 6 (THIOPHEN 2 YL) 1,2,4 TRIAZINE 5 OL; 3 (ALLYLSULFANYL) 6 METHYL 1,2,4 TRIAZINE 5 OL; ANTIBIOTIC AGENT; ATH 18534; ATH 25956; BACTERIUM LIPOPOLYSACCHARIDE; CEFOTAXIME; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBACTERIAL ACTIVITY; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIAL VIRULENCE; CARBOHYDRATE SYNTHESIS; CONTROLLED STUDY; CYTOTOXICITY; DRUG BLOOD LEVEL; DRUG EFFICACY; DRUG MECHANISM; DRUG MEGADOSE; DRUG RESEARCH; DRUG STRUCTURE; FEMALE; GRAM NEGATIVE INFECTION; GROWTH INHIBITION; HUMAN; HUMAN CELL; IN VITRO STUDY; KLEBSIELLA PNEUMONIAE; LOW DRUG DOSE; MINIMUM INHIBITORY CONCENTRATION; MOUSE; NONHUMAN; PNEUMONIA; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; TETRAHYMENA PYRIFORMIS;

ANIMALS; ANTI-BACTERIAL AGENTS; BACTERIA; CEFOTAXIME; CEFTIZOXIME; CYCLOPHOSPHAMIDE; DISEASE MODELS, ANIMAL; FEMALE; KLEBSIELLA INFECTIONS; KLEBSIELLA PNEUMONIAE; LUNG; MICE; MICE, INBRED BALB C; MOLECULAR STRUCTURE; MUTATION; NEUTROPENIA; PSEUDOMONAS AERUGINOSA; TETRAHYMENA PYRIFORMIS; TIME FACTORS; TRIAZINES; VIRULENCE;

ANIMALIA; BACTERIA (MICROORGANISMS); KLEBSIELLA; KLEBSIELLA PNEUMONIAE; PSEUDOMONAS AERUGINOSA; TETRAHYMENA PYRIFORMIS;

EID: 34047245106     PISSN: 14625814     EISSN: 14625822     Source Type: Journal    
DOI: 10.1111/j.1462-5822.2006.00877.x     Document Type: Article

References (25)

1. Alksne, L.E., and Projan, S.J. (2000) Bacterial virulence as a target for antimicrobial chemotherapy. Curr Opin Biotechnol 11: 625-636.
2. Arakawa, Y., Ohta, M., Wacharotayankun, R., Mori, M., Kido, N., Ito, H., et al. (1991) Biosynthesis of Klebsiella K2 capsular polysaccharide in Escherichia coli HB101 requires the functions of rmpA and the chromosomal cps gene cluster of the virulent strain Klebsiella pneumoniae Chedid (O1:K2). Infect Immun 59: 2043-2050.
3. Benghezal, M., Fauvarque, M.O., Tournebize, R., Froquet, R., Marchetti, A., Bergeret, E., et al. (2006) Specific host genes required for the killing of Klebsiella bacteria by phagocytes. Cell Microbiol 8: 139-148.
4. Bratu, S., Mooty, M., Nichani, S., Landman, D., Gullans, C., Pettinato, B., et al. (2005) Emergence of KPC-possessing Klebsiella pneumoniae in Brooklyn, New York: Epidemiology and recommendations for detection. Antimicrob Agents Chemother 49: 3018-3020.
5. Casadevall, A., and Pirofski, L.A. (1999) Host-pathogen interactions: redefining the basic concepts of virulence and pathogenicity. Infect Immun 67: 3703-3713.
6. Charette, S.J., and Cosson, P. (2004) Preparation of genomic DNA from Dictyostelium discoideum for PCR analysis. Biotechniques 36: 574-575.
7. Cosson, P., Zulianello, L., Join-Lambert, O., Faurisson, F., Gebbie, L., Benghezal, M., et al. (2002) Pseudomonas aeruginosa virulence analyzed in a Dictyostelium discoideum host system. J Bacteriol 184: 3027-3033.
8. Fang, C.T., Chuang, Y.P., Shun, C.T., Chang, S.C., and Wang, J.T. (2004) A novel virulence gene in Klebsiella pneumoniae strains causing primary liver abscess and septic metastatic complications. J Exp Med 199: 697-705.
9. Ferraro, M.J. (2003) Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. Approved standard. M7-A6, 5th edn. Villanova, PA: National Committee for Clinical Laboratory Standards (NCCLS).
10. Giacometti, A., Cirioni, O., Ghiselli, R., Dell'Acqua, G., Orlando, F., D'Amato, G., et al. (2005) RNAIII-inhibiting peptide improves efficacy of clinically used antibiotics in a murine model of staphylococcal sepsis. Peptides 26: 169-175.
11. Hentzer, M., Wu, H., Andersen, J.B., Riedel, K., Rasmussen, T.B., Bagge, N., et al. (2003) Attenuation of Pseudomonas aeruginosa virulence by quorum sensing inhibitors. EMBO J 22: 3803-3815.
12. Hung, D.T., Shakhnovich, E.A., Pierson, E., and Mekalanos, J.J. (2005) Small-molecule inhibitor of Vibrio cholerae virulence and intestinal colonization. Science 310: 670-674.
13. Izquierdo, L., Merino, S., Coderch, N., Regue, M., and Tomas, J.M. (2002) The wavB gene of Vibrio cholerae and the waaE of Klebsiella pneumoniae codify for a beta-1,4-glucosyltransferase involved in the transfer of a glucose residue to the 1-glycero-D-manno-heptose I in the lipopolysaccharide inner core. FEMS Microbiol Lett 216: 211-216.
14. Kauppi, A.M., Nordfelth, R., Uvell, H., Wolf-Watz, H., and Elofsson, M. (2003) Targeting bacterial virulence: Inhibitors of type III secretion in Yersinia. Chem Biol 10: 241-249.
15. Nordfelth, R., Kauppi, A.M., Norberg, H.A., Wolf-Watz, H., and Elofsson, M. (2005) Small-molecule inhibitors specifically targeting type III secretion. Infect Immun 73: 3104-3114.
16. Orci, L., Like, A.A., Amherdt, M., Blondel, B., Kanazawa, Y., Marliss, E.B., et al. (1973) Monolayer cell culture of neonatal rat pancreas: An ultrastructural and biochemical study of functioning endocrine cells. J Ultrastruct Res 43: 270-297.
17. Panchal, R.G., Hermone, A.R., Nguyen, T.L., Wong, T.Y., Schwarzenbacher, R., Schmidt, J., et al. (2004) Identification of small molecule inhibitors of anthrax lethal factor. Nat Struct Mol Biol 11: 67-72.
18. Podschun, R., and Ullmann, U. (1998) Klebsiella spp. as nosocomial pathogens: Epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin Microbiol Rev 11: 589-603.
19. Pradel, E., and Ewbank, J.J. (2004) Genetic models in pathogenesis. Annu Rev Genet 38: 347-363.
20. Roychoudhury, S., Zielinski, N.A., Ninfa, A.J., Allen, N.E., Jungheim, L.N., Nicas, T.I., and Chakrabarty, A.M. (1993) Inhibitors of two-component signal transduction systems: Inhibition of alginate gene activation in Pseudomonas aeruginosa. Proc Natl Acad Sci USA 90: 965-969.
21. Saenz, H.L., and Dehio, C. (2005) Signature-tagged mutagenesis: technical advances in a negative selection method for virulence gene identification. Curr Opin Microbiol 8: 612-619.
22. Schlimme, W., Marchiani, M., Hanselmann, K., and Jenni, B. (1999) BACTOX, a rapid bioassay that uses protozoa to assess the toxicity of bacteria. Appl Environ Microbiol 65: 2754-2757.
23. Tomas, J.M., Camprubi, S., and Williams, P. (1988) Surface exposure of the O-antigen in Klebsiella pneumoniae O1:K1 serotype strains. Microb Pathog 5: 141-147.
24. Turk, B.E., Wong, T.Y., Schwarzenbacher, R., Jarrell, E.T., Leppla, S.H., Collier, R.J., et al. (2004) The structural basis for substrate and inhibitor selectivity of the anthrax lethal factor. Nat Struct Mol Biol 11: 60-66.
25. Wright, J.S., III, Jin, R., and Novick, R.P. (2005) Transient interference with staphylococcal quorum sensing blocks abscess formation. Proc Natl Acad Sci USA 102: 1691-1696.