A recombinase system facilitates cloning of expression cassettes in the ciliate Tetrahymena thermophila

BMC Microbiology

Volumn 7, Issue , 2007, Pages

  Weide, Thomas ^a   Bockau, Ulrike ^b   Rave, Angelika ^b   Herrmann, Lutz ^b,c   Hartmann, Marcus W W ^b  

^a UNIVERSITY HOSPITAL MÜNSTER   (Germany)

^b UNIVERSITY OF MÜNSTER   (Germany)

^c Provendis GmbH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BLASTICIDIN S; CRE RECOMBINASE; MEROZOITE SURFACE PROTEIN 1; PROTEIN ANTIBODY; PROTOZOAL PROTEIN; 2 PYRROLIDONE DERIVATIVE; BLASTICIDIN A; COMPLEMENTARY DNA; INTEGRASE; PROTOZOAL DNA; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ANTIBODY SPECIFICITY; ARTICLE; EUKARYOTIC FLAGELLUM; EXPRESSION VECTOR; GENE CASSETTE; GENE EXPRESSION; GENE TRANSFER; GENETIC CODE; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PLASMID; PLASMODIUM FALCIPARUM; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTOZOAL GENETICS; TETRAHYMENA THERMOPHILA; WESTERN BLOTTING; ANIMAL; DRUG EFFECT; DRUG RESISTANCE; EVALUATION; GENE VECTOR; GENETIC RECOMBINATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; METHODOLOGY;

CILIOPHORA; PLASMODIUM FALCIPARUM; PROTISTA; TETRAHYMENA THERMOPHILA;

ANIMALS; CLONING, MOLECULAR; DNA, COMPLEMENTARY; DNA, PROTOZOAN; DRUG RESISTANCE; EVALUATION STUDIES; GENETIC VECTORS; INTEGRASES; MEROZOITE SURFACE PROTEIN 1; PLASMIDS; PLASMODIUM FALCIPARUM; PROTOZOAN PROTEINS; PYRROLIDINONES; RECOMBINATION, GENETIC; TETRAHYMENA THERMOPHILA;

EID: 34047175251     PISSN: 14712180     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-7-12     Document Type: Article

References (33)

1. Tetrahymena thermophila. K Collins MA Gorovsky, Curr Biol 2005 15 R317 R318 10.1016/j.cub.2005.04.039 15886083
2. Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote. JA Eisen RS Coyne M Wu D Wu M Thiagarajan JR Wortman JH Badger Q Ren P Amedeo KM Jones LJ Tallon AL Delcher SL Salzberg JC Silva BJ Haas WH Majoros M Farzad JM Carlton RK Smith Jr. J Garg RE Pearlman KM Karrer L Sun G Manning NC Elde AP Turkewitz DJ Asai DE Wilkes Y Wang H Cai K Collins BA Stewart SR Lee K Wilamowska Z Weinberg WL Ruzzo D Wloga J Gaertig J Frankel CC Tsao MA Gorovsky PJ Keeling RF Waller NJ Patron JM Cherry NA Stover CJ Krieger TC del HF Ryder SC Williamson RA Barbeau EP Hamilton E Orias, PLoS Biol 2006 4 e286 16933976 10.1371/journal.pbio.0040286
3. Functional genomics: the coming of age for Tetrahymena thermophila. AP Turkewitz E Orias G Kapler, Trends Genet 2002 18 35 40 10.1016/S0168-9525(01) 02560-4 11750699
4. Ribozymes, the first 20 years. TR Cech, Biochem Soc Trans 2002 30 1162 1166 10.1042/BST0301162 12440996
5. The dynein heavy chain family. DJ Asai DE Wilkes, J Eukaryot Microbiol 2004 51 23 29 10.1111/j.1550-7408.2004.tb00157.x 15068262
6. Manipulating dynein genes in Tetrahymena. DJ Asai KM DeWall LM Lincoln RK Smith, Methods Mol Biol 2001 161 269 278 11190512
7. Tetrahymena histone acetyltransferase A: a homolog to yeast Gcn5p linking histone acetylation to gene activation. JE Brownell J Zhou T Ranalli R Kobayashi DG Edmondson SY Roth CD Allis, Cell 1996 84 843 851 10.1016/S0092-8674(00)81063-6 8601308
8. Programmed DNA deletion as an RNA-guided system of genome defense. MC Yao P Fuller X Xi, Science 2003 300 1581 1584 10.1126/science.1084737 12791996
9. RNA-guided DNA deletion in Tetrahymena: an RNAi-based mechanism for programmed genome rearrangements. MC Yao JL Chao, Annu Rev Genet 2005 39 537 559 10.1146/annurev.genet.39.073003.095906 16285871
10. Tetrahymena: a model for growth, cell cycle and nutritional studies, with biotechnological potential. DN Wheatley L Rasmussen A Tiedtke, Bioessays 1994 16 367 372 10.1002/bies.950160512 8024545
11. Continuous high-cell-density fermentation of the ciliated protozoon Tetrahymena in a perfused bioreactor. T Kiy A Tiedtke, Appl Microbiol Biotechnol 1992 38 141 146 10.1007/BF00174458 1369137
12. Surface display of a parasite antigen in the ciliate Tetrahymena thermophila. J Gaertig Y Gao T Tishgarten TG Clark HW Dickerson, Nat Biotechnol 1999 17 462 465 10.1038/8638 10331805
13. Tetrahymena genetics: two nuclei are better than one. KM Karrer, Methods Cell Biol 2000 62 127 186 10503190
14. Tetrahymena Genome Database - TGB. 2007 http://www.ciliate.org
15. Germline and somatic transformation of mating Tetrahymena thermophila by particle bombardment. D Cassidy-Hanley J Bowen JH Lee E Cole LA VerPlank J Gaertig MA Gorovsky PJ Bruns, Genetics 1997 146 135 147 9136007
16. Tandem repeats of the 5' non-transcribed spacer of Tetrahymena rDNA function as high copy number autonomous replicons in the macronucleus but do not prevent rRNA gene dosage regulation. WJ Pan EH Blackburn, Nucleic Acids Res 1995 23 1561 1569 7784211 10.1093/nar/23.9.1561
17. Small RNAs in genome rearrangement in Tetrahymena. K Mochizuki MA Gorovsky, Curr Opin Genet Dev 2004 14 181 187 10.1016/j.gde.2004.01.004 15196465
18. Bacteriophage P1 site-specific recombination. Purification and properties of the Cre recombinase protein. K Abremski R Hoess, J Biol Chem 1984 259 1509 1514 6319400
19. The Flp double cross system a simple efficient procedure for cloning DNA fragments. PD Sadowski, BMC Biotechnol 2003 3 9 12871598 10.1186/1472-6750-3-9
20. GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes. AJ Walhout GF Temple MA Brasch JL Hartley MA Lorson HS van den M Vidal, Methods Enzymol 2000 328 575 592 11075367
21. Dynamic, structural, and regulatory aspects of lambda site-specific recombination. A Landy, Annu Rev Biochem 1989 58 913 949 2528323
22. The bifunctional dihydrofolate reductase thymidylate synthase of Tetrahymena thermophila provides a tool for molecular and biotechnology applications. L Herrmann U Bockau A Tiedtke MW Hartmann T Weide, BMC Biotechnol 2006 6 21 16549005 10.1186/1472-6750-6-21
23. Topography of epitopes on a polymorphic schizont antigen of Plasmodium falciparum determined by the binding of monoclonal antibodies in a two-site radioimmunoassay. CF Wilson R Anand JT Clark JS McBride, Parasite Immunol 1987 9 737 746 2448732
24. Fragments of the polymorphic Mr 185,000 glycoprotein from the surface of isolated Plasmodium falciparum merozoites form an antigenic complex. JS McBride HG Heidrich, Mol Biochem Parasitol 1987 23 71 84 10.1016/0166-6851(87)90189-7 2437453
25. Protozoan Genomes. 2007 http://www.sanger.ac.uk/Projects/Protozoa
26. Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia. JM Aury O Jaillon L Duret B Noel C Jubin BM Porcel B Segurens V Daubin V Anthouard N Aiach O Arnaiz A Billaut J Beisson I Blanc K Bouhouche F Camara S Duharcourt R Guigo D Gogendeau M Katinka AM Keller R Kissmehl C Klotz F Koll MA Le G Lepere S Malinsky M Nowacki JK Nowak H Plattner J Poulain F Ruiz V Serrano M Zagulski P Dessen M Betermier J Weissenbach C Scarpelli V Schachter L Sperling E Meyer J Cohen P Wincker, Nature 2006 444 171 178 10.1038/nature05230 17086204
27. Genome sequence of the human malaria parasite Plasmodium falciparum. MJ Gardner N Hall E Fung O White M Berriman RW Hyman JM Carlton A Pain KE Nelson S Bowman IT Paulsen K James JA Eisen K Rutherford SL Salzberg A Craig S Kyes MS Chan V Nene SJ Shallom B Suh J Peterson S Angiuoli M Pertea J Allen J Selengut D Haft MW Mather AB Vaidya DM Martin AH Fairlamb MJ Fraunholz DS Roos SA Ralph GI McFadden LM Cummings GM Subramanian C Mungall JC Venter DJ Carucci SL Hoffman C Newbold RW Davis CM Fraser B Barrell, Nature 2002 419 498 511 10.1038/nature01097 12368864
28. Electroporation-mediated replacement of a positively and negatively selectable beta-tubulin gene in Tetrahymena thermophila. J Gaertig TH Thatcher L Gu MA Gorovsky, Proc Natl Acad Sci U S A 1994 91 4549 4553 7910408 10.1073/pnas.91.10.4549
29. The Apicomplexan Database Resources - ApiDB. 2007 http://apidb.org/apidb
30. The Plasmodium Genome Resource - PlasmoD. 2007 http://www.plasmodb.org/ plasmo/home.jsp
31. The circumsporozoite protein of Plasmodium falciparum is expressed and localized to the cell surface in the free-living ciliate Tetrahymena thermophila. DS Peterson Y Gao K Asokan J Gaertig, Mol Biochem Parasitol 2002 122 119 126 10.1016/S0166-6851(02)00079-8 12106865
32. Cultivation of Tetrahymena thermophila in a 1.5-m3 airlift bioreactor. D Hellenbroich U Valley T Ryll R Wagner N Tekkanat W Kessler A Ross WD Deckwer, Appl Microbiol Biotechnol 1999 51 447 455 10.1007/s002530051415 10341428
33. Secretion of functional human enzymes by Tetrahymena thermophila. T Weide L Herrmann U Bockau N Niebur I Aldag W Laroy R Contreras A Tiedtke MW Hartmann, BMC Biotechnol 2006 6 19 16542419 10.1186/1472-6750-6-19