Plant regeneration via somatic embryogenesis using secondary needles of mature trees of Pinus roxburghii Sarg

International Journal of Botany

Volumn 3, Issue 1, 2007, Pages 40-47

  Malabadi, Ravindra B ^a,b   Nataraja, K ^a  

^a KARNATAK UNIVERSITY   (India)

^b FINNISH FOREST RESEARCH INSTITUTE   (Finland)

Author keywords

Chir pine; Micropropagation; Secondary needles; Stress; Triacontanol

Indexed keywords

CONIFEROPHYTA; PINUS ROXBURGHII;

EID: 34047171976     PISSN: 18119700     EISSN: 18119719     Source Type: Journal    
DOI: 10.3923/ijb.2007.40.47     Document Type: Article

References (58)

1. Arya, S., K. Kalia and I.D. Arya, 2000. Induction of somatic embryogenesis in Pinus roxburghii Sarg. Plant Cell Rep., 19: 775-780.
2. Biernbaum, J.A., R.L. Houtz and S.K. Ries, 1998. Field studies with crops treated with colloidally dispersed triacontanol. J. Am. Soc. Hortic. Sci., 113: 679-684.
3. Bonga, J.M. and S.E. Pond, 1991. Adventitious shoot formation in cultures of 30-year old Larix deciduas, L. leptolepis and L. laricina trees. Plant Cell Tiss. Org. Cult., 26: 45-51.
4. Bonga, J.M. and P. von Aderkas, 1993. Rejuvenation of Tissues from Mature Conifers and its Implications for Propagation in vitro. In: Clonal forestry I, Genetics and Biotechnology. Ahuja M.R. and W.J. Libby, (Eds.). Springer-Verlag, Berlin, Heidelberg, pp: 182-199.
5. Bonga, J.M., 1996. Frozen storage stimulates the formation of embryo-like structures and elongating shoots in explants from mature Larix deciduas and L. x eurolepos. Plant Cell Tiss. Org. Cult., 51: 195-200.
6. Bonga, J.M., 2004. The effect of various culture media on the formation of embryo-like structures in cultures derived from explants taken from mature Larix deciduas. Plant Cell Tiss. Org. Cult., 77: 43-48.
7. Burza, W. and S. Malepszy, 1995. In vitro culture of Cucumis sativus L. plants from protoplasts through direct somatic embryogenesis. Plant Cell Tiss. Org. Cult., 41: 259-266.
8. Deb, C.R. and P. Tandon, 2002. Induction of somatic embryogenesis in Khasi pine (Pinus kesiya) from secondary needles. J. Plant Biol., 29: 113-118.
9. Deb, C.R. and P. Tandon, 2004a. Establishment of an embryogenic suspension culture of Pinus kesiya (Khasi pine) from various explants. Ind. J. Biotechnol., 3: 445-448.
10. Deb, C.R. and P. Tandon, 2004b. Factors influencing initiation of embryogenic cultures in Pinus kesiya Royle ex. Gord. Ind. J. Biotechnol., 3: 589-593.
11. Dijak, M., D.L. Smith, T.J. Wilson and D.C.W. Brown, 1986. Stimulation of direct somatic embryogenesis from mesophyll protoplasts of Medicago sativa. Plant Cell Rep., 5: 468-470.
12. Fraternale, D., D. Giamperi, D. Ricci and M.B.L. Rocchi, 2002. Microprpagation of Bupleurum fruticosum L. The effect of triacontanol. Plant Cell Tiss. Org. Cult., 69: 135-140.
13. Gupta, P.K. and D.J. Durzan, 1985. Shoot multiplication from mature trees of Douglas fir and sugar pine. Plant Cell Rep., 4: 177-179.
14. Halperin, W. and D.R. Wetherell, 1965. Ammonium requirement for embryogenesis in vitro. Nature, 205: 519-520.
15. Janeiro, L.V., A. Ballester and A.M. Vieitez, 1995. Effect of cold storage on somatic embryogenesis systems of Camellia. J. Hortic. Sci., 70: 665-672.
16. Joy, R.W., D.D. Mc Intyre, H.J. Vogel and T.A. Thorpe, 1996. Stage specific nitrogen metabolism in developing carrot somatic embryos. Physiol Plant., 97: 149-159.
17. Kamada, H., T. Kobayashi, T. Kiyosue and H. Harada, 1989. Stress induced somatic embryogenesis in carrot and its application to synthetic seed production. In vitro Cell Dev. Biol. Plant., 25: 1163-1166.
18. Kiyosue, T., H. Kamada and H. Harada, 1989. Induction of somatic embryogenesis by salt stress in carrot. Plant Tiss. Cult. Letter., 6: 162-164.
19. Kiyosue, T., H. Kamada and H. Harada, 1990. Induction of somatic embryogenesis in carrot by heavy metal ions. Can. J. Bot., 68: 2301-2303.
20. Krul, W.R., 1993. Enhancement and repression of somatic embryogenesis in cell cultures of carrot by cold pretreatment of stock plants. Plant Cell Tiss. Org. Cult., 32: 271-276.
21. Leifert, C., K.P. Murphy and P.J. Lumsden, 1995. Mineral and carbohydrate nutrition of plant cell and tissue cultures. Crit. Rev. Plant Sci., 14: 83-109.
22. Malabadi, R.B., H. Choudhury and P. Tandon, 2002. Plant regeneration via somatic embryogenesis in Pinus kesiya (Royle ex. Gord). Applied. Biol. Res., 4: 1-10.
23. Malabadi, R.B. and J. van Staten, 2003. Somatic embryos can be induced from shoot apical domes of mature Pinus patula trees. S. Af. J. Bot., 69: 450-451.
24. Malabadi, R.B., H. Choudhury and P. Tandon, 2003. Effect of gelling agent, carbon sources and sterilization methods on initiation and establishment of embryogenic cultures in Khasi pine (Pinus kesiya Royle ex. Gord). Applied Biol. Res., 5: 1-8.
25. Malabadi, R.B., A.V. Ramarosandratana and J. van Staden, 2003b. Somatic embryogenesis from shoot apical domes of mature Pinus patula. Fifth Annual Conference of the Research Centre for Plant Growth and Development (RCPGD), University of KwaZulu-Natal, Pietermaritzburg, South Africa, 13-14th November, (Abstract); 28.
26. Malabadi, R.B., H. Choudhury and P. Tandon, 2004a. Initiation, maintenance and maturation of somatic embryos from thin apical dome sections in Pinus kesiya (Royle ex. Gord) promoted by partial desiccation and Gellan gum. Sci. Hortic., 102: 449-459.
27. Malabadi, R.B., A.V. Ramarosandratana and J. van Staden, 2004b. Recent advances in somatic embryogenesis of Pinus patula. S. Af. J. Bot., 70: 343.
28. Malabadi, R.B. and J. van Staden, 2004. Optimized somatic embryogenesis in Pinus patula. Sixth Annual conference of the Research Centre for Plant Growth and Development (RCPGD), University of KwaZulu-Natal, Pietermaritzburg, South Africa, 18-19th November, (Abstract); 20.
29. Malabadi, R.B., G.S. Mulgund and K. Nataraja, 2005. Plant regeneration via somatic embryogenesis in Pinus kesiya (Royle ex. Gord.) influenced by triacontanol. Acta Physiol. Plant., 27: 531-537.
30. Malabadi, R.B. and J. van Staden, 2005a. Somatic embryogenesis from vegetative shoot apices of mature trees of Pinus patula. Tree Physiol., 25: 11-16.
31. Malabadi, R.B. and J. van Staden, 2005b. Role of antioxidants and amino acids on somatic embryogenesis of Pinus patula. In vitro Cell. Dev. Biol-Plant., 41: 181-186.
32. Malabadi, R.B. and J. van Staden, 2005c. Storability and germination of sodium alginate encapsulated somatic embryos derived from the vegetative shoot apices of mature Pinus patula trees. Plant Cell Tiss. Org. Cult., 82: 259-265.
33. Malabadi, R.B. and J. van Staden, 2005d. Breakthrough in Forest biotechnology, University of KwaZulu-Natal, Pietermaritzburg, South Africa News Paper, March 2: 3.
34. Malabadi, R.B. and J. van Staden, 2006. Cold-enhanced somatic embryogenesis in Pinus patula is mediated by calcium. S. Af. J. Bot., 72: 613-618.
35. Malabadi, R.B., P.N. Hills and J. van Staden, 2006. RAPD assessment of clonal identity of somatic seedlings derived from the vegetative shoot apices of mature Pinus patula trees. S. Af. J. Bot., 72: 181-183.
36. Malabadi, R.B. and K. Nataraja, 2006a. Cryopreservation and plant regeneration via somatic embryogenesis using shoot apical domes of mature Pinus roxburghii Sarg. Trees. In vitro Cell. Dev. Biol. Plant., 42: 152-159.
37. Malabadi, R.B. and K. Nataraja, 2006b. RAPD detect no somaclonal variation in cryopreserved cultures of Pinus roxburghii SARG. Prop. Orn. Plants., 6: 114-120.
38. Malabadi, R.B. and K. Nataraja, 2007. Smoke saturated water influences somatic embryogenesis using vegetative shoot apices of mature trees of Pinus wallichiana A. B. Jacks. J. Plant Sci., 2: 45-53.
39. Mathur, G., S. von Arnold and R. Nadagouda, 2000. Studies on somatic embryogenesis from immature zygotic embryos of Chir pine (Pinus roxburghii Sarg.) Curr. Sci., 79: 999-1004.
40. Nakano, M. and M. Mii, 1993. Antibiotics stimulate somatic embryogenesis without plant growth regulators in several Dianthus cultivars. J. Plant Physiol., 141: 721-725.
41. Pechan, P.H. and W.A. Keller, 1989. Induction of microspore embryogenesis in Brassica napus L. gamma irradiation and ethanol stress. In vitro Cell Dev. Biol-Plant., 25: 1073-1074.
42. 3 as a replacement of NAA in the medium. Plant Sci., 129: 101-106.
43. Preece, J.E., 1995. Can nutrient salts partially substitute for plant growth regulators? Plant Tiss. Cult. Biotechnol., 1: 26-37.
44. Reddy, B.O., P. Giridhar and G.A. Ravishankar, 2002. The effect of triacontanol on micropropagation of Capsicum frutescens and Decalepis hamiltonii W and A. Plant Cell Tiss. Org. Cult., 71: 253-258.
45. Ries, S.K., V.F. Wert, C.C. Sweeley and R.A. Leavitt, 1977. Triacontanol: A new naturally occurring plant growth regulator. Science, 195: 1339-1341.
46. Ries, S.K. and V.F. Wert, 1982. Rapid in vivo and in vitro effects of triacontanol. J. Plant Growth Regul., 1: 117-127.
47. Ries, S.K. and V.F. Wert, 1988. Rapid elicitation of second messengers by nanomolar doses of triacontanol and octacosanol. Planta, 173: 79-87.
48. Ruaud, J.N., J. Bercetche and M. Paques, 1992. First evidence of somatic embryogenesis from needles of 1-year-old Picea abies plants. Plant Cell Rep., 11: 563-566.
49. Ruaud, J.N., 1993. Maturation and conversion into plantlets of somatic embryos derived from needles and cotyledons of 7-56-day-old Picea abies. Plant Sci., 92: 213-220.
50. Reinert, J., M. Tazawa and S. Semenoff, 1967. Nitrogen compounds as factors of embryogenesis in vitro. Nature, 216: 1215-1216.
51. Smith, D.L. and A.D. Krikonan, 1990. Low external pH replaces 2-4, D in maintaining and multiplying 2-4, D initiated embryogenic cells of carrot. Physiol Plant., 70: 329-336.
52. Smith, D.R., 1997. The role of in vitro methods in pine plantation establishment: The lesson from New Zealand. Plant Tiss. Cult. Biotechnol., 3: 63-73.
53. Tantos, A., Meszaros, A and J. Kissimon, 1999. The effect of triacontanol on micropropagation of balm, Melissa officinalis L. Plant Cell Rep., 19: 88-91.
54. Tantos, A., A. Meszaros, J. Kissimon, G. Horvath and T. Farkas, 2001. Triacontanol-supported microproprapagation of two woody plants. Plant Cell Rep., 19: 88-91.
55. Tamaszewski, J.Z., A.I. Kuklin, C.E. Sams and B.V. Conger, 1994. Influence of low temperature preincubation on somatic embryogenesis and ethylene emanation from orchard grass leaves. Plant Growth Regul., 14: 229-234.
56. Thorpe, T.A. and C. Stasolla, 2001. Somatic Embryogenesis. In: Current Trends in the Embryology of Angiosperms. Bhojwani, S.S. and W.H. Soh (Eds.). Kluwer Acad. Publishers, Dordrecht, pp: 279-336.
57. Westcott, R.J., 1994. Production of embryogenic callus from nonembryonic explants of Norway spruce Picea abies (L.) Karst. Plant Cell Rep., 14: 47-49.
58. Xie, J.H., M.W. Gao, Z.Q. Liang, Q.Y. Shu, X.Y. Cheng and Q.Z. Xue, 1997. The effect of cold-pretreatment on the isolated microspore culture and the free amino acid change of anthers in Japonica Rice (Orysa sativa L.) J. Plant Physiol., 151: 79-82.