An easy method for generating deletion mutants in Agrobacterium tumefaciens using a simple replacement vector

ScienceAsia

Volumn 31, Issue 4, 2005, Pages 349-357

  Suksomtip, Maneewan ^a   Tungpradabkul, Sumalee ^a  

^a MAHIDOL UNIVERSITY   (Thailand)

Author keywords

A. tumefaciens; citrate synthase gene; deletion mutants

Indexed keywords

EID: 34047116072     PISSN: 15131874     EISSN: None     Source Type: Journal    
DOI: 10.2306/scienceasia1513-1874.2005.31.349     Document Type: Article

References (16)

1. Herring CD, Glasner JD and Blattner FR (2003) Gene replacement without selection: regulated suppression of amber mutations in E. coli. Gene 331, 153-63.
2. Hoang TT, Karkhoff-Schweizer RR, Kutchma AJ and Schweizer HP (1998) A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 212, 77-86.
3. Sambrook J, and Russell DW (2001) Molecular cloning - A Laboratory Manual, third edition. pp 6.4 - 6.11. Cold Spring Harbor, New York.
4. Innis MA and Gelfane DH (1990) Optimization of PCRs. In: PCR protocols; A Guide to Methods and Application. (Edited by Innis MA, Gelfane DH, Sninsky JJ and White TJ). pp 3-11. San Diego Academic press, California.
5. Balbás P, Soberón X, Merino E, Zurita M, Lomeli Z, Valle F, Flores N and Bolivar F (1986) Plasmid vector pBR322 and its special purpose derivatives-a review. Gene 50, 3-40.
6. Sonnen H, Thierbach G, Kautz S, Kalinowski J, Schneider J, Pühler A and Kutzner R (1991) Characterization of pGA1, a new plasmid from Corynebacterium glutamicum LP-6. Gene 107, 69-74.
7. Schweizer HP (1992) Allelic exchange in Pseudomonas aeruginosa using novel ColE1-type vectors and a family of cassettes containing a portable oriT and the counter-selectable Bacillus subtilis sacB marker. Mol. Microbiol. 6, 1195-204.
8. Schweizer H.P (1991a) Improved broad –host-range lacbased plasmid vectors for the isolation and characterization of protein fusions in Pseudomonas aeruginosa. Gene 103, 87-92.
9. Hamilton CM, Aldea M, Washburn BK, Babitzke P and Kushner SR (1989) New method for generating deletions and gene replacements in Escherichia coli. J. Bacteriol. 171, 4617-22.
10. Blomfield IC, Vaughn V, Rest RF and Eisenstein BI, (1991) Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC 101 replicon. Mol. Microbiol. 5, 1447-57.
11. Link AJ, Phillips D and Church GM (1997) Methods for generating precise deletions and insertions in the genome of wild – type Escherichia coli: application to open reading frame characterization. J. Bacteriol. 179, 6228-37.
12. Martinez-Morales F, Borges AC, Martinez A, Shanmugam KT and Ingram LO (1999) Chromosomal integration of heterologous DNA in Escherichia coli with precise removal of markers and replicons used during construction. J. Bacteriol. 181, 7143-8.
13. Posfai G, Kolisnychenko V, Bereczki Z and Blattner FR (1999) Markerless gene replacement in Escherichia coli stimulated by a double-strand break in the chromosome. Nucleic Acids Res. 27, 4409-15.
14. Campbell A (1962) Episomes Adv. in Genet. 11, 101-9.
15. Chen L, Chen Y, Wood DW and Nester EW (2002) A new type IV secretion system promotes conjugal transfer in Agrobacterium tumefaciens. J. Bacteriol. 184), 4838-45.
16. Schweizer HP and Hoang TT (1995) An improved system for gene replacement and xylE fusion analysis in Pseudomonas aeruginosa. Gene 158, 15-22.