3D localized photoactivation of pa-GFP in living cells using two-photon interactions

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

Volumn , Issue , 2006, Pages 389-391

  Diaspro, Alberto ^a,b   Testa, Ilaria ^b   Faretta, Mario ^c   Magrassi, Raffaella ^b   Barozzi, Sara ^c   Parazzoli, Dario ^c   Vicidomini, Giuseppe ^b  

^a IEEE   (Italy)

^b UNIVERSITY OF GENOA   (Italy)

^c FIRC INSTITUTE OF MOLECULAR ONCOLOGY   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

AEQUOREA VICTORIA GREEN FLUORESCENT PROTEIN (; MOLECULAR SWITCHING; PHOTOACTIVATION;

CELLS; CHROMOPHORES; FLUORESCENCE; MEDICAL IMAGING; MOLECULAR DYNAMICS; PHOTONS; PROTEIN FOLDING;

LIVING SYSTEMS STUDIES;

GREEN FLUORESCENT PROTEIN;

ARTICLE; CONFOCAL MICROSCOPY; EVALUATION STUDY; HELA CELL LINE; HUMAN; ILLUMINATION; IMAGE ENHANCEMENT; LIGHT; METABOLISM; METHODOLOGY; MULTIPHOTON MICROSCOPY; RADIATION EXPOSURE; THREE DIMENSIONAL IMAGING;

GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; IMAGE ENHANCEMENT; IMAGING, THREE-DIMENSIONAL; LIGHT; LIGHTING; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE, MULTIPHOTON;

EID: 34047114001     PISSN: 05891019     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1109/IEMBS.2006.259967     Document Type: Conference Paper

References (10)

1. Tsien, R. Y. "The Green Fluorescent Protein", Annual Review of Biochemistry, 67, 509, 1998.
2. Patterson, G. H.; Lippincott-Schwarz, J. "A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells", Science, 297, 1873, 2002.
3. Lippincott-Schwartz, J.; Altan-Bonnet, N.; Patterson, G. H. "Photobleaching and photoactivation: following protein dynamics in living cells", Nat Cell Biol, Suppl, S7, 2003.
4. Miyawaki, A. "Fluorescent proteins in a new light", 22, 1374, 2004.
5. Patterson, G. H.; Lippincott-Schwartz, J. "Selective photolabeling of proteins using photo activatable GFP", Methods, 32, 445, 2004.
6. Diaspro A. (ed) Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances; Wiley-Liss: New York, 2001, pp 567.
7. Schneider M, Barozzi S, Testa I, Faretta M, Diaspro A. "Two-photon activation and excitation properties of PA-GFP in the 720-920-nm region." Biophys. J. 89: 1346-1352, 2005.
8. Diaspro A.; Chirico G.; Collini M. "Two-photon fluorescence excitation and related techniques in biological microscopy", Quarterly Reviews of Biophysics, 38, 1-72, 2005.
9. Post, J. N.; Lidke, K. A.; Rieger, B.; Arndt-Jovin, D. J. "Photoactivation of PAGFP in live Drosophila embryos: two-photon activation and phototoxicity", FEBS Letters, 579, 325, 2005.
10. Chirico G, Diaspro A, Cannone F, Collini M, Bologna S, Pellegrini V, Beltram F. "Selective fluorescence recovery after bleaching of single E(2)GFP proteins induced by two-photon excitation." Chemphyschem. 6(2):328-335, 2005.