In vitro exposure to 0.57-MHz electric currents exerts cytostatic effects in HepG2 human hepatocarcinoma cells

International Journal of Oncology

Volumn 30, Issue 3, 2007, Pages 583-592

  Hernández Bule, María Luisa ^a   Trillo, María Ángeles ^a   Cid, María Antonia ^a   Leal, Jocelyne ^a   Úbeda, Alejandro ^a  

^a HOSPITAL RAMÓN Y CAJAL   (Spain)

Author keywords

Capacitive resistive therapy; Cytostasis; Electric currents; HepG2; Non thermal

Indexed keywords

BROXURIDINE; CYCLINE;

ARTICLE; BIOSYNTHESIS; CELL CYCLE; CELL PROLIFERATION; ELECTRIC CONDUCTIVITY; ELECTRICITY; ELECTROCHEMISTRY; ELECTROMAGNETIC FIELD; ELECTROSTIMULATION THERAPY; FLUORESCENCE MICROSCOPY; HUMAN; LIVER CELL CARCINOMA; LIVER TUMOR; METHODOLOGY; TIME; TUMOR CELL LINE;

BROMODEOXYURIDINE; CARCINOMA, HEPATOCELLULAR; CELL CYCLE; CELL LINE, TUMOR; CELL PROLIFERATION; ELECTRIC CONDUCTIVITY; ELECTRIC STIMULATION THERAPY; ELECTRICITY; ELECTROCHEMISTRY; ELECTROMAGNETIC FIELDS; HUMANS; LIVER NEOPLASMS; MICROSCOPY, FLUORESCENCE; PROLIFERATING CELL NUCLEAR ANTIGEN; TIME FACTORS;

EID: 33947712278     PISSN: 10196439     EISSN: 17912423     Source Type: Journal    
DOI: 10.3892/ijo.30.3.583     Document Type: Article

References (41)

1. Mondardini P, Tanzi R, Verardi L, Briglia S, Maione A and Draco E: Nuove metodología nel trattamento della patología muscolare traumática dell'atleta. Med Sport 52: 201-213, 1999.
2. Ganzit GP, Stefanini L and Stesina G: Nuove methodice nei trattamento della patología muscolo-articolare dell'atleta: la tercaterapia. Med Sport 53: 361-367, 2000.
3. Parolo E and Onesta MP: HCR:900: Ipertermia a transferimento energetico resistivo e capacitivo nel trattamento di lesioni músculo-scheletriche actue a croniche. Riabilitazione 2: 81-83, 1998. (Pubitemid 28405668)
4. Melegati G, Volpi P, Tornese D and Mele G: Rehabilitación en patologías del tendón (Excerpta). Revista del Deporte, Investigación de traumatología e investigaciones afines 21: 66-83, 1999.
5. Martínez-Rubio A and Bordas-Serrat JR: Tratamiento del asma mediante Transferencia Eléctrica Capacitiva (TEC). Rehabil Fis XXI 3: 18-19, 1992.
6. Calpe J, García C, Hernández R, Gaspar M and Rotellar E: Nuestra experiencia con un recuperador electrónico en lesiones vasculares de pacientes hemodializados. Rev Soc Esp Dial Trasplant 19: 19-22, 1998.
7. Ley A, Cladellas M, Colet P, De las Heras P, Florensa J, Prim J, Roussos J, Ariza A and Calbet J: Transferencia eléctrica capacitiva (TEC). Técnica no invasiva de hipertermia profunda en el tratamiento de los gliomas cerebrales. Resultados preliminares. Neurocirugía 3: 118-123, 1992.
8. Ley A: Hipertermia intracraneal no invasiva con Transferencia Eléctrica Capacitiva (TEC), Termometría cerebral y intratumoral. Neurocirugía 14: 41-452, 2003.
9. Ley A, Ariza A and Rosell R: Tratamiento quirúrgico de los gliomas malignos. Hipertermia. In: Tumores del Sistema Nervioso Central. Doyma (ed). Barcelona, pp55-64, 1993.
10. Guix R and Guix JR: Hipertermia en oncología. Medic-Delfos: Revista Científica del Centro Médico Delfos 1: 20-23, 1997.
11. Van der Zee J: Heating the patient: a promising approach? (Review) Ann Oncol 13: 1173-1184, 2002.
12. Wust P, Hildebrandt B, Sreenivasa G, Rau B, Gellermann J, Riess H, Felix R and Schlag PM: Hyperthermia in combined treatment of cancer (Review). Lancet Oncol 3: 487-497, 2002.
13. Hildebrandt B, Wust P, Ahlers O, Dieing A, Sreenivasa G, Kerner T, Felix R and Riess H: The cellular and molecular basis of hyperthermia. Crit Rev Oncol Hematol 43: 33-56, 2002.
14. Zeng ZC, Jiang GL, Wang GM, Tang ZY, Curran WJ and Iliakis G: DNA-PKcs subunits in radiosensitization by hyperthermia on hepatocellular carcinoma HepG2 cell line. World J Gastroenterol 8: 797-803, 2002.
15. Hernández-Bule ML, Trillo MA, Bazán E, Martínez- Pascual MA, Leal J and Úbeda A: Niveles atérmicos de corrientes eléctricas usadas en terapia por trasferencia eléctrica capacitiva inducen efectos citotóxicos parciales en cultivos de neuroblastoma humano. Neurocirugía 15: 366-371, 2004.
16. Domínguez-Malagón H and Gaytan-Graham S: Hepatocellular carcinoma: an update (Review). Ultrastruct Pathol 25: 497-516, 2001.
17. Liu C-L and Fan S-T: Non-resectional therapies for hepatocellular carcinoma. Am J Surg 173: 356-365, 1997.
18. Wang E, Yin Y, Zhao M, Forrester JV and McCaig CD: Physiological electric fields control the G1/S phase cell cycle checkpoint to inhibit endothelial cell proliferation. FASEB J 17: 458-460, 2003.
19. Stacey M, Stickley J, Fox P, Statler V, Schoenbach K, Beebe SJ and Buescher S: Differential effects in cells exposed to ultrashort, high intensity electric fields: cell survival, DNA damage and cell cycle analysis. Mutat Res 542: 65-75, 2003.
20. Beebe SJ, Fox PM, Rec LJ, Willis EL and Schoenbach KH: Nanosecond, high-intensity pulsed electric fields induce apoptosis in human cell. FASEB J 17: 1493-1495, 2003.
21. Kirson ED, Gurvich Z, Schneiderman R, Dekel E, Itzhaki A, Wasserman Y, Schatzberger R and Palti Y: Disruption of cancer cell replication by alternating electric fields. Cancer Res 64: 3288-3295, 2004.
22. Radeva M and Berg H: Differences in lethality between cancer cells and human lymphocytes caused by LF-electromagnetic fields. Bioelectromagnetics 25: 503-507, 2004.
23. Gimsa J, Habel B, Schreiber U, van Rienen U, Strauss U and Gimsa U: Choosing electrodes for deep brain stimulation experiments-electrochemical considerations. J Neurosci Methods 142: 251-265, 2005. (Pubitemid 40215459)
24. Tennant JR: Evaluation of the Trypan blue technique for determination of cell viability. Transplantation 2: 685-694, 1964.
25. Lowry OH, Rosebrough NT, Farr AL and Randall RJ: Protein measurement with the Folin reagent. J Biol Chem 193: 256-275, 1951.
26. Burton K: Study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 62: 315-323, 1956.
27. Nakano A, Watanabe N, Nishizaki Y, Takashimizu S and Matsuzaki S: Immunohistochemical studies on expression of Pglycoprotein and p53 in relation to histological differentiation and cells proliferation in hepatocellular carcinoma. Hepatol Res 25: 158-165, 2003. (Pubitemid 36416665)
28. Woods AL, Hall PA, Shepherd NA, Hanby AM, Waseem NH, Lane DP and Levison DA: The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S+G2+M phase fraction (flow cytometric analysis) and prognosis. Histopathology 19: 21-27, 1991.
29. Bradford MM: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye-binding. Anal Biochem 72: 248-254, 1976.
30. Turler A, Schaefer H, Schaefer N, Wagner M, Maintz D and Qiao JC: Experimental low-level direct current therapy in liver metastases: influence of polarity and current dose. Bioelectromagnetics 21: 395-401, 2000.
31. Xin YL: Advances in the treatment of malignant tumours by electrochemical therapy (ECT). Eur J Surg Suppl 574: 31-35, 1994. (Pubitemid 24309186)
32. Korestein R, Somjen D, Fischler H and Binderman I: Capacitative pulsed electric stimulation of bone cells induction of cyclic-AMP changes and DNA synthesis. Biochim Biophys Acta 803: 302-307, 1984. (Pubitemid 14138205)
33. Noda M, Jonson DE, Chiabrera A and Rodan GA: Effect of electric currents on DNA synthesis in rat osteosarcoma cells: dependence on conditions that influence cell growth. J Orthop Res 5: 253-260, 1987. (Pubitemid 17586947)
34. Bourguignon GJ and Bourguignon LY: Electric stimulation of proteins and DNA synthesis in human fibroblasts. FASEB J 1: 398-402, 1987. (Pubitemid 18033760)
35. Ozawa H, Abe E, Shibasaki Y, Fukuhara T and Suda T: Electric fields stimulate DNA synthesis of mouse osteoblast-like cells (MC3T3-E1) by a mechanism involving calcium ions. J Cell Physiol 138: 477-483, 1989. (Pubitemid 19083765)
36. Long Y, Liu H, Guan Z, He X and Cai G: Effects of pulsed electric fields on DNA synthesis in an osteoblast-like cell line UMR-106. Tsinghua Sci Technol 5: 439-442, 2000.
37. Cheng K and Goldman RJ: Electric fields and proliferation in a dermal wound model: cell cycle kinetics. Bioelectromagnetics 19: 68-74, 1998.
38. Goldman R and Pollack S: Electric fields and proliferation in chronic wound model. Bioelectromagnetics 17: 450-457, 1996.
39. Gillett CE and Barnes DM: Demystified cell cycle. J Clin Pathol Mol Pathol 51: 310-316, 1998.
40. Berg H: Problems of weak electromagnetic field effects in cell biology. Bioelectrochem Bioenerg 48: 355-360, 1999.
41. Binhi VN and Goldman RJ: Ion-protein dissociation predicts 'windows' in electric field-induced wound-cell proliferation. Biochim Biophys Acta 1474: 147-156, 2000.