Improvement of the sensitivity and resolution of PCR-SSCP analysis with optimized primer concentrations in PCR products

Journal of Genetics

Volumn 85, Issue 3, 2006, Pages 233-235

  Zhu, Xilin ^a   Niu, Nifang ^a   Liu, Yang ^a   Du, Te ^a   Chen, Dongmei ^a   Wang, Xin ^a   Gu, Harvest F ^b   Liu, Ying ^a  

^a INSTITUTE OF BASIC MEDICAL SCIENCES   (China)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Mutation screening; PCR; Primer; Single strand conformation polymorphism

Indexed keywords

CALCITRIOL RECEPTOR; DNA; ENDODEOXYRIBONUCLEASE FOKI; HLA DR ANTIGEN; HLA-DRB1 ANTIGEN; PRIMER DNA; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE; UNCLASSIFIED DRUG;

ARTICLE; CHEMISTRY; DNA TEMPLATE; EXON; GENETICS; HUMAN; METHODOLOGY; MOLECULAR GENETICS; NUCLEIC ACID AMPLIFICATION; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROMOTER REGION; SENSITIVITY AND SPECIFICITY; SINGLE STRAND CONFORMATION POLYMORPHISM;

BASE SEQUENCE; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EXONS; HLA-DR ANTIGENS; HUMANS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PLASMIDS; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; PROMOTER REGIONS (GENETICS); RECEPTORS, CALCITRIOL; SENSITIVITY AND SPECIFICITY; TEMPLATES, GENETIC;

EID: 33947622530     PISSN: 00221333     EISSN: 00221333     Source Type: Journal    
DOI: 10.1007/BF02935339     Document Type: Article

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