Properties of recombinant glycine decarboxylase P- and H-protein subunits from the cyanobacterium Synechocystis sp. strain PCC 6803

FEBS Letters

Volumn 581, Issue 7, 2007, Pages 1297-1301

  Hasse, Dirk ^a   Mikkat, Stefan ^a   Thrun, Hans Albrecht ^a   Hagemann, Martin ^a   Bauwe, Hermann ^a  

^a UNIVERSITY OF ROSTOCK   (Germany)

Author keywords

Cyanobacteria; Glycine decarboxylase; H protein; P protein; Synechocystis

Indexed keywords

GLYCINE; GLYCINE CLEAVAGE SYSTEM H PROTEIN; GLYCINE DEHYDROGENASE (DECARBOXYLATING); RECOMBINANT ENZYME;

ARTICLE; BACTERIAL STRAIN; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SYNTHESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; SYNECHOCYSTIS;

DIMERIZATION; GLYCINE; GLYCINE DECARBOXYLASE COMPLEX H-PROTEIN; GLYCINE DEHYDROGENASE (DECARBOXYLATING); HYDROGEN-ION CONCENTRATION; PROTEIN SUBUNITS; RECOMBINANT PROTEINS; SYNECHOCYSTIS;

CYANOBACTERIA; SYNECHOCYSTIS; SYNECHOCYSTIS SP.;

EID: 33947429268     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2007.02.037     Document Type: Article

References (26)

1. Douce R., Bourguignon J., Neuburger M., and Rebeille F. The glycine decarboxylase system: a fascinating complex. Trends Plant Sci. 6 (2001) 167-176
2. Applegarth D.A., and Toone J.R. Non-ketotic hyperglycinemia (glycine encephalopathy): laboratory diagnosis. Mol. Genet. Metab. 74 (2001) 139-146
3. Somerville C.R., and Ogren W.L. Mutants of the cruciferous plant Arabidopsis thaliana lacking glycine decarboxylase activity. Biochem. J. 202 (1982) 373-380
4. Voll L.M., Jamai A., Renné P., Voll H., McClung C.R., and Weber A.P.M. The photorespiratory Arabidopsis shm1 mutant is deficient in SHM1. Plant Physiol. 140 (2006) 59-66
5. Hagemann M., Vinnemeier J., Oberpichler I., Boldt R., and Bauwe H. The glycine decarboxylase complex is not essential for the cyanobacterium Synechocystis sp. strain PCC 6803. Plant Biol. 7 (2005) 15-22
6. Eisenhut M., Kahlon S., Hasse D., Ewald R., Lieman-Hurwitz J., Ogawa T., Ruth W., Bauwe H., Kaplan A., and Hagemann M. The plant-like C2 glycolate pathway and the bacterial-like glycerate cycle cooperate in phosphoglycolate metabolism in cyanobacteria. Plant Physiol. 142 (2006) 333-342
7. Kochi H., and Kikuchi G. Mechanism of the reversible glycine cleavage reaction in Arthrobacter globiformis. I. Purification and function of protein components required for the reaction. J. Biochem. 75 (1974) 1113-1127
8. Oliver D.J., Neuburger M., Bourguignon J., and Douce R. Interaction between the component enzymes of the glycine decarboxylase multienzyme complex. Plant Physiol. 94 (1990) 833-839
9. Hiraga K., and Kikuchi G. The mitochondrial glycine cleavage system. Functional association of glycine decarboxylase and aminomethyl carrier protein. J. Biol. Chem. 255 (1980) 11671-11676
10. Guilhaudis L., Simorre J.P., Blackledge M., Marion D., Gans P., Neuburger M., and Douce R. Combined structural and biochemical analysis of the H-T complex in the glycine decarboxylase cycle: evidence for a destabilization mechanism of the H-protein. Biochemistry 39 (2000) 4259-4266
11. Neuburger M., Polidori A.M., Pietre E., Faure M., Jourdain A., Bourguignon J., Pucci B., and Douce R. Interaction between the lipoamide-containing H-protein and the lipoamide dehydrogenase (L-protein) of the glycine decarboxylase multienzyme system. 1. Biochemical studies. Eur. J. Biochem. 267 (2000) 2882-2889
12. Pares S., Cohen-Addad C., Sieker L., Neuburger M., and Douce R. X-ray structure determination at 2.6-Å resolution of a lipoate-containing protein: the H-protein of the glycine decarboxylase complex from pea leaves. Proc. Natl. Acad. Sci. USA 91 (1994) 4850-4853
13. Faure M., Bourguignon J., Neuburger M., Macherel D., Sieker L., Ober R., Kahn R., Cohen-Addad C., and Douce R. Interaction between the lipoamide-containing H-protein and the lipoamide dehydrogenase (L-protein) of the glycine decarboxylase multienzyme system. 2. Crystal structures of H- and L-proteins. Eur. J. Biochem. 267 (2000) 2890-2898
14. Okamura-Ikeda K., Hosaka H., Yoshimura M., Yamashita E., Toma S., Nakagawa A., Fujiwara K., Motokawa Y., and Taniguchi H. Crystal structure of human T-protein of glycine cleavage system at 2.0 Å resolution and its implication for understanding non-ketotic hyperglycinemia. J. Mol. Biol. 351 (2005) 1146-1159
15. Nakai T., Nakagawa N., Maoka N., Masui R., Kuramitsu S., and Kamiya N. Structure of P-protein of the glycine cleavage system: implications for non-ketotic hyperglycinemia. EMBO J. 24 (2005) 1536
16. Martin W., Rujan T., Richly E., Hansen A., Cornelsen S., Lins T., Leister D., Stoebe B., Hasegawa M., and Penny D. Evolutionary analysis of Arabidopsis, cyanobacterial, and chloroplast genomes reveals plastid phylogeny and thousands of cyanobacterial genes in the nucleus. Proc. Natl. Acad. Sci. USA 99 (2002) 12246-12251
17. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning: A Laboratory Manual (1989), Cold Spring Harbor Laboratory Press, New York
18. Hagemann M., Schoor A., Jeanjean R., Zuther E., and Joset F. The stpA gene from Synechocystis sp. strain PCC 6803 encodes the glucosylglycerol-phosphate phosphatase involved in cyanobacterial osmotic response to salt shock. J. Bacteriol. 179 (1997) 1727-1733
19. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
20. Klein S.M., and Sagers R.D. Glycine metabolism. I. Properties of the system catalyzing the exchange of bicarbonate with the carboxyl-group of glycine in Peptococcus glycinophilus. J. Biol. Chem. 241 (1966) 197-205
21. Berry S., Fischer J.H., Kruip J., Hauser M., and Wildner G.F. Monitoring cytosolic pH of carboxysome-deficient cells of Synechocystis sp PCC 6803 using fluorescence analysis. Plant Biol. 7 (2005) 342-347
22. Hiraga K., and Kikuchi G. The mitochondrial glycine cleavage system. Purification and properties of glycine decarboxylase from chicken liver mitochondria. J. Biol. Chem. 255 (1980) 11664-11670
23. Motokawa Y., and Kikuchi G. Glycine metabolism by rat liver mitochondria: reconstitution of the reversible glycine cleavage system with partially purified protein components. Arch. Biochem. Biophys. 164 (1974) 624-633
24. Sarojini G., and Oliver D.J. Extraction and partial characterization of the glycine decarboxylase multienzyme complex from pea leaf mitochondria. Plant Physiol. 72 (1983) 194-199
25. Kochi H., and Kikuchi G. Mechanism of reversible glycine cleavage reaction in Arthrobacter globiformis - function of lipoic acid in cleavage and synthesis of glycine. Arch. Biochem. Biophys. 173 (1976) 71-81
26. Macherel D., Bourguignon J., Forest E., Faure M., Cohen-Addad C., and Douce R. Expression, lipoylation and structure determination of recombinant pea H-protein in Escherichia coli. Eur. J. Biochem. 236 (1996) 27-33