Quantitative detection of micrometastases in pelvic lymph nodes in patients with clinically localized prostate cancer by real-time reverse transcriptase-PCR

Clinical Cancer Research

Volumn 13, Issue 4, 2007, Pages 1192-1197

  Miyake, Hideaki ^a   Hara, Isao ^a   Kurahashi, Toshifumi ^a   Inoue, Taka Aki ^b   Eto, Hiroshi ^b   Fujisawa, Masato ^a  

^a KOBE UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b HYOGO MEDICAL CENTER FOR ADULTS   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CANCER ANTIBODY; MESSENGER RNA; PROSTATE SPECIFIC ANTIGEN; PROSTATE SPECIFIC MEMBRANE ANTIGEN;

ARTICLE; CANCER DIAGNOSIS; CANCER LOCALIZATION; CANCER RECURRENCE; CANCER SURVIVAL; CELL ISOLATION; CONTROLLED STUDY; GLEASON SCORE; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; MICROMETASTASIS; PELVIS LYMPH NODE; PHYSICAL EXAMINATION; PRIORITY JOURNAL; PROSTATE CANCER; PROSTATECTOMY; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; TUMOR VOLUME;

ANTIGENS, SURFACE; FEMALE; GLUTAMATE CARBOXYPEPTIDASE II; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (PHOSPHORYLATING); HUMANS; LYMPH NODES; LYMPHATIC METASTASIS; MALE; PELVIS; PROSTATE-SPECIFIC ANTIGEN; PROSTATIC NEOPLASMS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 33947289616     PISSN: 10780432     EISSN: None     Source Type: Journal    
DOI: 10.1158/1078-0432.CCR-05-2706     Document Type: Article

References (21)

1. Walsh PC, Partin AW, Epstein JI. Cancer control and quality of life following anatomical radical retropubic prostatectomy: results at 10 years. J Urol 1994;152:1831-6.
2. Catalona WJ, Smith DS. Cancer recurrence and survival rates after anatomic radical retropubic prostatectomy for prostate cancer: intermediate-term results. J Urol 1998;160:2428-34.
3. Okegawa T, Nutahara K, Higashihara E. Detection of micrometastatic prostate cancer cells in the lymph nodes by reverse transcriptase polymerase chain reaction is predictive of biochemical recurrence in pathological stage T2 prostate cancer. J Urol 2000;163:1183-8.
4. Potter SR, Mangold LA, Shue MJ, et al. Molecular and immunohistochemical staging of men with seminal vesicle invasion and negative pelvic lymph nodes at radical prostatectomy. Cancer 2000;89:2577-86.
5. Ferrari AC, Stone NN, Eyler JN, et al. Prospective analysis of prostate-specific markers in pelvic lymph nodes of patients with high-risk prostate cancer. J Natl Cancer Inst 1997;89:1498-504.
6. Wawroschek F, Wagner T, Hamm M, et al. The influence of serial sections, immunohistochemistry, and extension of pelvic lymph node dissection on the lymph node status in clinically localized prostate cancer. Eur Urol 2003;43:132-7.
7. Gibson UE, Heid CA, Williams PM. A novel method for real time quantitative RT-PCR. Genome Res 1996;6:995-1001.
8. Van Trappen PO, Gyselman VG, Lowe DG, et al. Molecular quantification and mapping of lymph-node micrometastases in cervical cancer. Lancet 2001;357:15-20.
9. Mrras M, Mikhitarian K, Walters C, et al. Quantitative real-time RT-PCR detection of breast cancer micrometastasis using a multigene marker panel. Int J Cancer 2001;93:162-71.
10. Yoshioka S, Fujiwara Y, Sugita Y, et al. Real-time reverse transcriptase-polymerase chain reaction for intraoperative diagnosis of lymph node micrometastasis: clinical application for cervical lymph node dissection in esophageal cancers. Surgery 2002;132:34-40.
11. Kubota K, Nakanishi H, Hiki N, et al. Quantitative detection of micrometastases in the lymph nodes of gastric cancer patients with real-time RT-PCR: a comparative study with immunohistochemistry. Int J Cancer 2003;105:136-43.
12. Inokuchi M, Ninomiya I, Tsugawa K, Terada I, Miwa K. Quantitative evaluation of metastases in axillary lymph nodes of breast cancer. Br J Cancer 2003;89:1750-6.
13. Sobin LH, Wittekind CH, editors. TNM classification of malignant tumors. 5th ed. New York: Wiley-Liss; 1997.
14. Das H, Koizumi T, Sugimoto T, et al. Quantitation of Fas and Fas ligand gene expression in human ovarian, cervical and endometrial carcinomas using real-time quantitative RT-PCR. Br J Cancer 2000;82:1682-8.
15. Kurahashi T, Hara I, Oka N, Kamidono S, Eto H, Miyake H. Detection of micrometastases in pelvic lymph nodes in patients undergoing radical cystectomy for locally invasive bladder cancer by real-time reverse transcriptase-PCR for cytokeratin 19 and uroplakin II. Clin Cancer Res 2005;11:3773-7.
16. Kurahashi T, Muramaki M, Yamanaka K, Hara I, Miyake H. Expression of the secreted form of clusterin protein in renal cell carcinoma as a predictor of disease extension. BJU Int 2005;96:895-9.
17. Weingartner K, Ramaswamy A, Bittinger A, et al. Anatomical basis for pelvic lymphadenectomy in prostate cancer: results of an autopsy study and implications for the clinic. J Urol 1996;156:1969-73.
18. Bader P, Burkhard FC, Markwalder R, et al. Disease progression and survival of patients with positive lymph nodes after radical prostatectomy. Is there a chance of cure? J Urol 2003;169:849-54.
19. Cheng L, Zincke H, Blute ML,et al. Risk of prostate carcinoma death in patients with lymph node metastasis. Cancer 2001;91:66-73.
20. Haas CJ, Wagner T, Wawroschek F, Arnholdt H. Combined application of RT-PCR and immunohistochemistry on paraffin embedded sentinel lymph nodes of prostate cancer patients. Pathol Res Pract 2005;200:763-70.
21. Shariat SF, Kattan MW, Erdamar S, et al. Detection of clinically significant, occult prostate cancer metastases in lymph nodes using a splice variant-specific rt-PCR assay for human glandular kallikrein. J Clin Oncol 2003;21:1223-31.