Determination of angiotensin I-converting enzyme activity in cell culture using fluorescence resonance energy transfer peptides

Analytical Biochemistry

Volumn 363, Issue 2, 2007, Pages 255-262

  Sabatini, R A ^a   Bersanetti, P A ^a   Farias, S L ^a   Juliano, L ^a   Juliano, M A ^a   Casarini, D E ^a   Carmona, A K ^a   Paiva, A C M ^a   Pesquero, J B ^a  

^a FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Angiotensin I converting enzyme; Aorta smooth muscle cells (ASMC); Cell culture; CHO cells; Enzyme activity; Fluorescence resonance energy transfer (FRET) peptides; Fluorimetric assay

Indexed keywords

CELL CULTURE; CELLS; ENERGY TRANSFER; FORSTER RESONANCE ENERGY TRANSFER; HYDROLYSIS; MUSCLE; PEPTIDES; RESONANCE;

ANGIOTENSIN I-CONVERTING ENZYME; CHO CELL; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORIMETRIC ASSAYS; SMOOTH MUSCLE CELLS;

ENZYME ACTIVITY;

DIPEPTIDYL CARBOXYPEPTIDASE; DIPEPTIDYL CARBOXYPEPTIDASE INHIBITOR; LISINOPRIL; PEPTIDE DERIVATIVE;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL CULTURE; CELL MEMBRANE; CHO CELL; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME SUBSTRATE; FEMALE; FLUORESCENCE RESONANCE ENERGY TRANSFER; HYDROLYSIS KINETICS; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; RAT; SMOOTH MUSCLE FIBER;

CRICETULUS GRISEUS;

EID: 33947278905     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.01.020     Document Type: Article

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